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Binding of dietary Cobalt to sarcoplasmic reticulum proteins

Persson, B.; Carlenor, E.; Clyne, N. LU ; Hultman, E.; Lins, L. E.; Pehrsson, S. K. and Rydström, J. LU (1992) In Scandinavian Journal of Clinical & Laboratory Investigation 52(2). p.137-140
Abstract

It has previously been shown that cobalt accumulates in the myocardium of rats, mainly the sarcoplasmic reticulum (SR) and the mitochondrial inner membrane. In order to investigate the mode of accumulation of cobalt in the SR, rats were given a dietary cobalt supplementation of 40 mg of CoSO4 × 7 H2O kg-1 body wt, after which the rats were sacrificed and the sarcoplasmic reticulum was isolated. The SR proteins were subjected to analysis by polyacrylamide gel electrophoresis followed by protein staining and determination of the content of cobalt in each protein band. The major cobalt-binding protein was found to have a molecular weight of about 100 000; a 200 000 molecular weight protein was also found to... (More)

It has previously been shown that cobalt accumulates in the myocardium of rats, mainly the sarcoplasmic reticulum (SR) and the mitochondrial inner membrane. In order to investigate the mode of accumulation of cobalt in the SR, rats were given a dietary cobalt supplementation of 40 mg of CoSO4 × 7 H2O kg-1 body wt, after which the rats were sacrificed and the sarcoplasmic reticulum was isolated. The SR proteins were subjected to analysis by polyacrylamide gel electrophoresis followed by protein staining and determination of the content of cobalt in each protein band. The major cobalt-binding protein was found to have a molecular weight of about 100 000; a 200 000 molecular weight protein was also found to bind cobalt, although less extensively. These results suggest that cobalt is bound to the monomeric and dimeric forms of Ca2+-ATPase in the SR of the myocardium. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Congestive heart failure, Metal ion binding, Rat myocardium
in
Scandinavian Journal of Clinical & Laboratory Investigation
volume
52
issue
2
pages
4 pages
publisher
Informa Healthcare
external identifiers
  • Scopus:0026560892
ISSN
0036-5513
DOI
10.3109/00365519209088777
language
English
LU publication?
no
id
7a091075-6691-4590-a2bc-b2d01872ceda
date added to LUP
2016-08-30 22:04:57
date last changed
2016-09-16 15:09:50
@misc{7a091075-6691-4590-a2bc-b2d01872ceda,
  abstract     = {<p>It has previously been shown that cobalt accumulates in the myocardium of rats, mainly the sarcoplasmic reticulum (SR) and the mitochondrial inner membrane. In order to investigate the mode of accumulation of cobalt in the SR, rats were given a dietary cobalt supplementation of 40 mg of CoSO<sub>4</sub> × 7 H<sub>2</sub>O kg<sup>-1</sup> body wt, after which the rats were sacrificed and the sarcoplasmic reticulum was isolated. The SR proteins were subjected to analysis by polyacrylamide gel electrophoresis followed by protein staining and determination of the content of cobalt in each protein band. The major cobalt-binding protein was found to have a molecular weight of about 100 000; a 200 000 molecular weight protein was also found to bind cobalt, although less extensively. These results suggest that cobalt is bound to the monomeric and dimeric forms of Ca<sup>2+</sup>-ATPase in the SR of the myocardium. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.</p>},
  author       = {Persson, B. and Carlenor, E. and Clyne, N. and Hultman, E. and Lins, L. E. and Pehrsson, S. K. and Rydström, J.},
  issn         = {0036-5513},
  keyword      = {Congestive heart failure,Metal ion binding,Rat myocardium},
  language     = {eng},
  number       = {2},
  pages        = {137--140},
  publisher    = {ARRAY(0x90759a8)},
  series       = {Scandinavian Journal of Clinical & Laboratory Investigation},
  title        = {Binding of dietary Cobalt to sarcoplasmic reticulum proteins},
  url          = {http://dx.doi.org/10.3109/00365519209088777},
  volume       = {52},
  year         = {1992},
}