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A novel bio-electronic tongue using different cellobiose dehydrogenases to resolve mixtures of various sugars and interfering analytes.

Cipri, Andrea; Schulz, Christopher LU ; Ludwig, Roland; Gorton, Lo and Del Valle, Manel (2015) In Biosensors & Bioelectronics 79. p.515-521
Abstract
A novel application of cellobiose dehydrogenase (CDH) as sensing element for a Bioelectronic Tongue (BioET) system has been tested. In this work CDHs from various fungi, which exhibit different substrate specificities, were used to discriminate between lactose and glucose in presence of the interfering matrix compound Ca(2+) in various mixtures. This work exploits the advantage of an electronic tongue system with practically zero pre-treatment of samples and operation at low voltages in a direct electron transfer mode. The Artificial Neural Network (ANN) used in the BioET system to interpret the voltammetric data was able to provide a correct prediction of the concentrations of the analytes considered. Correlation coefficients in the... (More)
A novel application of cellobiose dehydrogenase (CDH) as sensing element for a Bioelectronic Tongue (BioET) system has been tested. In this work CDHs from various fungi, which exhibit different substrate specificities, were used to discriminate between lactose and glucose in presence of the interfering matrix compound Ca(2+) in various mixtures. This work exploits the advantage of an electronic tongue system with practically zero pre-treatment of samples and operation at low voltages in a direct electron transfer mode. The Artificial Neural Network (ANN) used in the BioET system to interpret the voltammetric data was able to provide a correct prediction of the concentrations of the analytes considered. Correlation coefficients in the comparison of obtained vs. expected concentrations were highly significant, especially for lactose (R(2)=0.975) and Ca(2+) (R(2)=0.945). This BioET application has a high potential especially for the food and dairy industry and also, if further miniaturised in screen printed format, for its in-situ use. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biosensors & Bioelectronics
volume
79
pages
515 - 521
publisher
Elsevier
external identifiers
  • PMID:26748369
  • Scopus:84952690561
  • WOS:000370309200072
ISSN
1873-4235
DOI
10.1016/j.bios.2015.12.069
language
English
LU publication?
yes
id
95e74a0d-4552-475b-a75b-bf1fc3e88634 (old id 8592514)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26748369?dopt=Abstract
date added to LUP
2016-02-10 11:34:21
date last changed
2016-10-30 04:33:36
@misc{95e74a0d-4552-475b-a75b-bf1fc3e88634,
  abstract     = {A novel application of cellobiose dehydrogenase (CDH) as sensing element for a Bioelectronic Tongue (BioET) system has been tested. In this work CDHs from various fungi, which exhibit different substrate specificities, were used to discriminate between lactose and glucose in presence of the interfering matrix compound Ca(2+) in various mixtures. This work exploits the advantage of an electronic tongue system with practically zero pre-treatment of samples and operation at low voltages in a direct electron transfer mode. The Artificial Neural Network (ANN) used in the BioET system to interpret the voltammetric data was able to provide a correct prediction of the concentrations of the analytes considered. Correlation coefficients in the comparison of obtained vs. expected concentrations were highly significant, especially for lactose (R(2)=0.975) and Ca(2+) (R(2)=0.945). This BioET application has a high potential especially for the food and dairy industry and also, if further miniaturised in screen printed format, for its in-situ use.},
  author       = {Cipri, Andrea and Schulz, Christopher and Ludwig, Roland and Gorton, Lo and Del Valle, Manel},
  issn         = {1873-4235},
  language     = {eng},
  pages        = {515--521},
  publisher    = {ARRAY(0xa8f3200)},
  series       = {Biosensors & Bioelectronics},
  title        = {A novel bio-electronic tongue using different cellobiose dehydrogenases to resolve mixtures of various sugars and interfering analytes.},
  url          = {http://dx.doi.org/10.1016/j.bios.2015.12.069},
  volume       = {79},
  year         = {2015},
}