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Production of human monoclonal antibodies in dialysis tubing

Sjogren-Jansson, E.; Ohlin, M. LU ; Borrebaeck, C. A K LU and Jeansson, S. (1991) In Hybridoma 10(3). p.411-419
Abstract

Human x mouse hybridoma cells were grown in dialysis tubing (DT) to obtain large quantities of human monoclonal antibodies (MAb). Hybridoma cells were grown inside the DT, which was placed in a tissue culture flask containing medium. The medium inside the DT was supplemented with different additives which may be selected depending on the intended use of the MAb. About 10-50 times higher concentrations of immunoglobulin (Ig) were obtained after cultivation in DT compared to conventional tissue culture (CTC) for 2 days. The purity of the MAb was high which facilitated further antibody purification. Production of human MAb in DT proved to be excellent for evaluation studies in laboratory scale. It does not require expensive equipment and... (More)

Human x mouse hybridoma cells were grown in dialysis tubing (DT) to obtain large quantities of human monoclonal antibodies (MAb). Hybridoma cells were grown inside the DT, which was placed in a tissue culture flask containing medium. The medium inside the DT was supplemented with different additives which may be selected depending on the intended use of the MAb. About 10-50 times higher concentrations of immunoglobulin (Ig) were obtained after cultivation in DT compared to conventional tissue culture (CTC) for 2 days. The purity of the MAb was high which facilitated further antibody purification. Production of human MAb in DT proved to be excellent for evaluation studies in laboratory scale. It does not require expensive equipment and several hybridomas can be grown simultaneously.

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author
organization
publishing date
type
Contribution to journal
publication status
published
in
Hybridoma
volume
10
issue
3
pages
9 pages
publisher
Mary Ann Liebert, Inc.
external identifiers
  • Scopus:0025828513
ISSN
0272-457X
language
English
LU publication?
yes
id
862dcc99-7450-442c-a6d2-e10f54ccf1ad
date added to LUP
2016-04-20 16:22:22
date last changed
2016-04-21 14:39:19
@misc{862dcc99-7450-442c-a6d2-e10f54ccf1ad,
  abstract     = {<p>Human x mouse hybridoma cells were grown in dialysis tubing (DT) to obtain large quantities of human monoclonal antibodies (MAb). Hybridoma cells were grown inside the DT, which was placed in a tissue culture flask containing medium. The medium inside the DT was supplemented with different additives which may be selected depending on the intended use of the MAb. About 10-50 times higher concentrations of immunoglobulin (Ig) were obtained after cultivation in DT compared to conventional tissue culture (CTC) for 2 days. The purity of the MAb was high which facilitated further antibody purification. Production of human MAb in DT proved to be excellent for evaluation studies in laboratory scale. It does not require expensive equipment and several hybridomas can be grown simultaneously.</p>},
  author       = {Sjogren-Jansson, E. and Ohlin, M. and Borrebaeck, C. A K and Jeansson, S.},
  issn         = {0272-457X},
  language     = {eng},
  number       = {3},
  pages        = {411--419},
  publisher    = {ARRAY(0x7757528)},
  series       = {Hybridoma},
  title        = {Production of human monoclonal antibodies in dialysis tubing},
  volume       = {10},
  year         = {1991},
}