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Hydrogen Peroxide Vapor Decontamination in a Patient Room Using Feline Calicivirus and Murine Norovirus as Surrogate Markers for Human Norovirus.

Holmdahl, Torsten LU ; Walder, Mats LU ; Uzcátegui, Nathalie; Odenholt, Inga LU ; Lanbeck, Peter LU ; Medstrand, Patrik LU and Widell, Anders LU (2016) In Infection Control & Hospital Epidemiology 37(5). p.561-566
Abstract
OBJECTIVE To determine whether hydrogen peroxide vapor (HPV) could be used to decontaminate caliciviruses from surfaces in a patient room. DESIGN Feline calicivirus (FCV) and murine norovirus (MNV) were used as surrogate viability markers to mimic the noncultivable human norovirus. Cell culture supernatants of FCV and MNV were dried in triplicate 35-mm wells of 6-well plastic plates. These plates were placed in various positions in a nonoccupied patient room that was subsequently exposed to HPV. Control plates were positioned in a similar room but were never exposed to HPV. METHODS Virucidal activity was measured in cell culture by reduction in 50% tissue culture infective dose titer for FCV and by both 50% tissue culture infective dose... (More)
OBJECTIVE To determine whether hydrogen peroxide vapor (HPV) could be used to decontaminate caliciviruses from surfaces in a patient room. DESIGN Feline calicivirus (FCV) and murine norovirus (MNV) were used as surrogate viability markers to mimic the noncultivable human norovirus. Cell culture supernatants of FCV and MNV were dried in triplicate 35-mm wells of 6-well plastic plates. These plates were placed in various positions in a nonoccupied patient room that was subsequently exposed to HPV. Control plates were positioned in a similar room but were never exposed to HPV. METHODS Virucidal activity was measured in cell culture by reduction in 50% tissue culture infective dose titer for FCV and by both 50% tissue culture infective dose titer and plaque reduction for MNV. RESULTS Neither viable FCV nor viable MNV could be detected in the test room after HPV treatment. At least 3.65 log reduction for FCV and at least 3.67 log reduction for MNV were found by 50% tissue culture infective dose. With plaque assay, measurable reduction for MNV was at least 2.85 log units. CONCLUSIONS The successful inactivation of both surrogate viruses indicates that HPV could be a useful tool for surface decontamination of a patient room contaminated by norovirus. Hence nosocomial spread to subsequent patients can be avoided. Infect. Control Hosp. Epidemiol. 2016;1-6. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Infection Control & Hospital Epidemiology
volume
37
issue
5
pages
561 - 566
publisher
University of Chicago Press
external identifiers
  • PMID:26861195
  • WOS:000374127300010
  • Scopus:84964299210
ISSN
0899-823X
DOI
10.1017/ice.2016.15
language
English
LU publication?
yes
id
c86cde8f-9740-4d1c-8695-a7576cf9509c (old id 8825991)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26861195?dopt=Abstract
date added to LUP
2016-03-03 16:27:58
date last changed
2016-11-21 11:28:00
@misc{c86cde8f-9740-4d1c-8695-a7576cf9509c,
  abstract     = {OBJECTIVE To determine whether hydrogen peroxide vapor (HPV) could be used to decontaminate caliciviruses from surfaces in a patient room. DESIGN Feline calicivirus (FCV) and murine norovirus (MNV) were used as surrogate viability markers to mimic the noncultivable human norovirus. Cell culture supernatants of FCV and MNV were dried in triplicate 35-mm wells of 6-well plastic plates. These plates were placed in various positions in a nonoccupied patient room that was subsequently exposed to HPV. Control plates were positioned in a similar room but were never exposed to HPV. METHODS Virucidal activity was measured in cell culture by reduction in 50% tissue culture infective dose titer for FCV and by both 50% tissue culture infective dose titer and plaque reduction for MNV. RESULTS Neither viable FCV nor viable MNV could be detected in the test room after HPV treatment. At least 3.65 log reduction for FCV and at least 3.67 log reduction for MNV were found by 50% tissue culture infective dose. With plaque assay, measurable reduction for MNV was at least 2.85 log units. CONCLUSIONS The successful inactivation of both surrogate viruses indicates that HPV could be a useful tool for surface decontamination of a patient room contaminated by norovirus. Hence nosocomial spread to subsequent patients can be avoided. Infect. Control Hosp. Epidemiol. 2016;1-6.},
  author       = {Holmdahl, Torsten and Walder, Mats and Uzcátegui, Nathalie and Odenholt, Inga and Lanbeck, Peter and Medstrand, Patrik and Widell, Anders},
  issn         = {0899-823X},
  language     = {eng},
  number       = {5},
  pages        = {561--566},
  publisher    = {ARRAY(0x9875b18)},
  series       = {Infection Control & Hospital Epidemiology},
  title        = {Hydrogen Peroxide Vapor Decontamination in a Patient Room Using Feline Calicivirus and Murine Norovirus as Surrogate Markers for Human Norovirus.},
  url          = {http://dx.doi.org/10.1017/ice.2016.15},
  volume       = {37},
  year         = {2016},
}