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Human antibody reactivity against the lower matrix protein (pp65) produced by cytomegalovirus

Ohlin, M. LU ; Plachter, B.; Sundqvist, V. A.; Steensbakkers, P. G A; Middeldorp, J. M. and Borrebaeck, C. A K LU (1995) In Clinical and Diagnostic Laboratory Immunology 2(3). p.325-329
Abstract

The lower matrix protein (pp65) is a major product of many laboratory strains of cytomegalovirus (CMV). It is thus an integral part of many CMV serological assays based on native antigen. Recombinant fragments of pp65 have previously been investigated for their usefulness in more-defined assays. The latter antigens have, however, failed to develop a positive response with serum samples derived from a substantial number of infected individuals. Here we show that the human humoral immune response to CMV pp65 is highly diverse and recognizes at least seven distinct but in some cases partly overlapping epitopes. Most of these epitopes could not be mimicked by any of the investigated recombinant or synthetic antigens. Furthermore, when we... (More)

The lower matrix protein (pp65) is a major product of many laboratory strains of cytomegalovirus (CMV). It is thus an integral part of many CMV serological assays based on native antigen. Recombinant fragments of pp65 have previously been investigated for their usefulness in more-defined assays. The latter antigens have, however, failed to develop a positive response with serum samples derived from a substantial number of infected individuals. Here we show that the human humoral immune response to CMV pp65 is highly diverse and recognizes at least seven distinct but in some cases partly overlapping epitopes. Most of these epitopes could not be mimicked by any of the investigated recombinant or synthetic antigens. Furthermore, when we investigated the ability of human CMV-seropositive serum samples to block the reactivity of pp65 specific antibodies recognizing five different epitopes within pp65, it was evident that several sera did not contain significant levels of antibodies against any of these or overlapping structures. It was thus concluded that the antibody response against CMV pp65 is weak in some CMV-infected individuals, making this antigen unsuitable for use alone in serological screening systems for CMV infection.

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author
organization
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type
Contribution to journal
publication status
published
in
Clinical and Diagnostic Laboratory Immunology
volume
2
issue
3
pages
5 pages
publisher
American Society for Microbiology
external identifiers
  • Scopus:0029018218
ISSN
1071-412X
language
English
LU publication?
yes
id
911802f2-786f-46b2-ab8e-36cbd59f58d4
date added to LUP
2016-04-19 14:12:09
date last changed
2016-04-22 12:56:07
@misc{911802f2-786f-46b2-ab8e-36cbd59f58d4,
  abstract     = {<p>The lower matrix protein (pp65) is a major product of many laboratory strains of cytomegalovirus (CMV). It is thus an integral part of many CMV serological assays based on native antigen. Recombinant fragments of pp65 have previously been investigated for their usefulness in more-defined assays. The latter antigens have, however, failed to develop a positive response with serum samples derived from a substantial number of infected individuals. Here we show that the human humoral immune response to CMV pp65 is highly diverse and recognizes at least seven distinct but in some cases partly overlapping epitopes. Most of these epitopes could not be mimicked by any of the investigated recombinant or synthetic antigens. Furthermore, when we investigated the ability of human CMV-seropositive serum samples to block the reactivity of pp65 specific antibodies recognizing five different epitopes within pp65, it was evident that several sera did not contain significant levels of antibodies against any of these or overlapping structures. It was thus concluded that the antibody response against CMV pp65 is weak in some CMV-infected individuals, making this antigen unsuitable for use alone in serological screening systems for CMV infection.</p>},
  author       = {Ohlin, M. and Plachter, B. and Sundqvist, V. A. and Steensbakkers, P. G A and Middeldorp, J. M. and Borrebaeck, C. A K},
  issn         = {1071-412X},
  language     = {eng},
  number       = {3},
  pages        = {325--329},
  publisher    = {ARRAY(0xa3be8b0)},
  series       = {Clinical and Diagnostic Laboratory Immunology},
  title        = {Human antibody reactivity against the lower matrix protein (pp65) produced by cytomegalovirus},
  volume       = {2},
  year         = {1995},
}