Advanced

6;7 chromosomal translocation in spontaneously arising rat immunocytomas : evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target

Pear, W S; Wahlström, G; Nelson, S F; Axelson, H LU ; Szeles, A; Wiener, F; Bazin, H; Klein, G and Sümegi, J (1988) In Molecular and Cellular Biology 8(1). p.441-51
Abstract

Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sümegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlström, J. Szpirer, G. Levan, G. Klein, and J. Sümegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping... (More)

Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sümegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlström, J. Szpirer, G. Levan, G. Klein, and J. Sümegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping of the c-myc breakpoints showed that in 10 of 14 tumors, the c-myc breakpoints are clustered in a 1.5-kilobase region upstream of exon 1. In contrast with sporadic Burkitt's lymphoma and mouse plasmacytoma, only 1 of 14 tumors contains the c-myc breakpoints in either exon 1 or intron 1. Analysis of the sequences juxtaposed to the c-myc show that immunoglobulin H switch regions are the targets in at least five tumors and that there is a strong correlation between the secreted immunoglobulin and the c-myc target. Unlike sporadic Burkitt's lymphoma and mouse plasmacytoma, at least two rat immunocytomas show recombination of the c-myc with sequences distinct from immunoglobulin switch regions.

(Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Base Sequence, Chromosome Mapping, DNA, Neoplasm, Gene Expression Regulation, Genes, Immunoglobulin, Genes, Switch, Immunoglobulin Constant Regions, Immunoglobulin Heavy Chains, Immunoglobulin Isotypes, Karyotyping, Molecular Sequence Data, Nucleic Acid Hybridization, Plasmacytoma, Proto-Oncogene Proteins, Rats, Translocation, Genetic
in
Molecular and Cellular Biology
volume
8
issue
1
pages
11 pages
publisher
American Society for Microbiology
external identifiers
  • Scopus:3543039008
ISSN
0270-7306
language
English
LU publication?
no
id
99f4ef20-aa85-4330-bbc2-ef3da8ed784c
date added to LUP
2016-08-09 09:21:50
date last changed
2016-09-15 10:00:49
@misc{99f4ef20-aa85-4330-bbc2-ef3da8ed784c,
  abstract     = {<p>Our previous studies have shown that spontaneously arising immunocytomas in the LOU/Ws1 strain of rats contain a t(6;7) chromosomal translocation in all seven tumors studied (F. M. Babonits, J. Spira, G. Klein, and H. Bazin, Int. J. Cancer 29:431-437, 1982). We have also shown that the c-myc is located on chromosome 7 (J. Sümegi, J. Spira, H. Bazin, J. Szpirer, G. Levan, and G. Klein, Nature (London) 306:497-499, 1983) and the immunoglobulin H cluster on chromosome 6 (W.S. Pear, G. Wahlström, J. Szpirer, G. Levan, G. Klein, and J. Sümegi, Immunogenetics 23:393-395, 1986). We now report a detailed cytogenetic and molecular analysis of nine additional rat immunocytomas. The t(6;7) chromosomal translocation is found in all tumors. Mapping of the c-myc breakpoints showed that in 10 of 14 tumors, the c-myc breakpoints are clustered in a 1.5-kilobase region upstream of exon 1. In contrast with sporadic Burkitt's lymphoma and mouse plasmacytoma, only 1 of 14 tumors contains the c-myc breakpoints in either exon 1 or intron 1. Analysis of the sequences juxtaposed to the c-myc show that immunoglobulin H switch regions are the targets in at least five tumors and that there is a strong correlation between the secreted immunoglobulin and the c-myc target. Unlike sporadic Burkitt's lymphoma and mouse plasmacytoma, at least two rat immunocytomas show recombination of the c-myc with sequences distinct from immunoglobulin switch regions.</p>},
  author       = {Pear, W S and Wahlström, G and Nelson, S F and Axelson, H and Szeles, A and Wiener, F and Bazin, H and Klein, G and Sümegi, J},
  issn         = {0270-7306},
  keyword      = {Animals,Base Sequence,Chromosome Mapping,DNA, Neoplasm,Gene Expression Regulation,Genes, Immunoglobulin,Genes, Switch,Immunoglobulin Constant Regions,Immunoglobulin Heavy Chains,Immunoglobulin Isotypes,Karyotyping,Molecular Sequence Data,Nucleic Acid Hybridization,Plasmacytoma,Proto-Oncogene Proteins,Rats,Translocation, Genetic},
  language     = {eng},
  number       = {1},
  pages        = {441--51},
  publisher    = {ARRAY(0x8d363c8)},
  series       = {Molecular and Cellular Biology},
  title        = {6;7 chromosomal translocation in spontaneously arising rat immunocytomas : evidence for c-myc breakpoint clustering and correlation between isotypic expression and the c-myc target},
  volume       = {8},
  year         = {1988},
}