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Immunoglobulin production induced by CD57+ GC-derived helper T cells in vitro requires addition of exogenous IL-2

Andersson, Eva LU ; Dahlenborg, Katarina LU ; Ohlin, Mats LU ; Borrebaeck, Carl A K LU and Carlsson, Roland LU (1996) In Cellular Immunology 169(2). p.166-173
Abstract

Germinal centers (GC) are well-defined areas in lymphoid organs were B cells proliferate and differentiate in response to T-cell-dependent antigens. The GC comprises B cells, follicular dendritic cells, tangible body macrophages, and a low number of CD4+ T cells. A large portion of these T cells expresses CD57. We have examined the ability of the CD4+CD57+ GC T cells to become activated and to take part in B cell activation processes, These T cells coexpress CD45RO, CD69, CD28, and upon mitogenic stimulation CD25, The cell population was found neither to containe nor to be able to produce any specific mRNA for IL-2, IL-4, and IFN-γ upon activation. Levels of mRNA encoding CD40 ligand was also... (More)

Germinal centers (GC) are well-defined areas in lymphoid organs were B cells proliferate and differentiate in response to T-cell-dependent antigens. The GC comprises B cells, follicular dendritic cells, tangible body macrophages, and a low number of CD4+ T cells. A large portion of these T cells expresses CD57. We have examined the ability of the CD4+CD57+ GC T cells to become activated and to take part in B cell activation processes, These T cells coexpress CD45RO, CD69, CD28, and upon mitogenic stimulation CD25, The cell population was found neither to containe nor to be able to produce any specific mRNA for IL-2, IL-4, and IFN-γ upon activation. Levels of mRNA encoding CD40 ligand was also undetectable under similar conditions. Furthermore, in contrast to ordinary CD4+ T cells, this population expressing CD57 was unable to induce B cells to Ig production in the presence of pokeweed mitogen or SEA unless IL-2 was added to the cultures, However, despite their apparent lack of function CD4+CD57+ GC T cells were found to rescue GC B cells from cell death in vitro to the same extent as CD4+CD57- T(h) cells, The phenotypical and functional differences found between these T cells and regular T(h)-cells suggest that they either represent a T cell subset with distinct properties within the GC yet to be determined or that they represent T cells, Irate in the immune response, having lost most of their original functions and capabilities.

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author
organization
publishing date
type
Contribution to journal
publication status
published
in
Cellular Immunology
volume
169
issue
2
pages
8 pages
publisher
Elsevier
external identifiers
  • Scopus:0029943961
ISSN
0008-8749
DOI
10.1006/cimm.1996.0107
language
English
LU publication?
yes
id
bd692bd6-222d-48e1-aa6b-3a360ba2a97f
date added to LUP
2016-04-19 14:08:35
date last changed
2016-05-16 14:49:18
@misc{bd692bd6-222d-48e1-aa6b-3a360ba2a97f,
  abstract     = {<p>Germinal centers (GC) are well-defined areas in lymphoid organs were B cells proliferate and differentiate in response to T-cell-dependent antigens. The GC comprises B cells, follicular dendritic cells, tangible body macrophages, and a low number of CD4<sup>+</sup> T cells. A large portion of these T cells expresses CD57. We have examined the ability of the CD4<sup>+</sup>CD57<sup>+</sup> GC T cells to become activated and to take part in B cell activation processes, These T cells coexpress CD45RO, CD69, CD28, and upon mitogenic stimulation CD25, The cell population was found neither to containe nor to be able to produce any specific mRNA for IL-2, IL-4, and IFN-γ upon activation. Levels of mRNA encoding CD40 ligand was also undetectable under similar conditions. Furthermore, in contrast to ordinary CD4<sup>+</sup> T cells, this population expressing CD57 was unable to induce B cells to Ig production in the presence of pokeweed mitogen or SEA unless IL-2 was added to the cultures, However, despite their apparent lack of function CD4<sup>+</sup>CD57<sup>+</sup> GC T cells were found to rescue GC B cells from cell death in vitro to the same extent as CD4<sup>+</sup>CD57<sup>-</sup> T(h) cells, The phenotypical and functional differences found between these T cells and regular T(h)-cells suggest that they either represent a T cell subset with distinct properties within the GC yet to be determined or that they represent T cells, Irate in the immune response, having lost most of their original functions and capabilities.</p>},
  author       = {Andersson, Eva and Dahlenborg, Katarina and Ohlin, Mats and Borrebaeck, Carl A K and Carlsson, Roland},
  issn         = {0008-8749},
  language     = {eng},
  month        = {05},
  number       = {2},
  pages        = {166--173},
  publisher    = {ARRAY(0xa25fd98)},
  series       = {Cellular Immunology},
  title        = {Immunoglobulin production induced by CD57+ GC-derived helper T cells in vitro requires addition of exogenous IL-2},
  url          = {http://dx.doi.org/10.1006/cimm.1996.0107},
  volume       = {169},
  year         = {1996},
}