Advanced

The effects of plasmin and protein Ca on factor VIII:C and VIII:CAg

Holmberg, Lars LU ; Ljung, R LU and Nilsson, Inga Marie (1983) In Thrombosis Research 31(1). p.41-50
Abstract

The effects of various concentrations of plasmin and activated protein C on the factor VIII procoagulant activity (VIII:C) and coagulant antigen (VIII:CAg) were studied in factor VIII concentrates and normal plasma. Small amounts (0.1 CTA U/ml) of plasmin rapidly destroyed VIII:C, and affected, but did not destroy VIII:CAg, in factor VIII concentrates. In normal plasma larger amounts of plasmin (1.8 CTA U/ml) was required to inactivate VIII:C in order to exceed the neutralizing capacity of alpha 2-antiplasmin. VIII:CAg was unchanged indicating a limited proteolysis. The difference between VIII:C and VIII:CAg was found also in urokinase-activated plasma. Activated protein C (5 micrograms/ml), in the presence of Ca2+ and phospholipids,... (More)

The effects of various concentrations of plasmin and activated protein C on the factor VIII procoagulant activity (VIII:C) and coagulant antigen (VIII:CAg) were studied in factor VIII concentrates and normal plasma. Small amounts (0.1 CTA U/ml) of plasmin rapidly destroyed VIII:C, and affected, but did not destroy VIII:CAg, in factor VIII concentrates. In normal plasma larger amounts of plasmin (1.8 CTA U/ml) was required to inactivate VIII:C in order to exceed the neutralizing capacity of alpha 2-antiplasmin. VIII:CAg was unchanged indicating a limited proteolysis. The difference between VIII:C and VIII:CAg was found also in urokinase-activated plasma. Activated protein C (5 micrograms/ml), in the presence of Ca2+ and phospholipids, inactivated VIII:C without affecting VIII:CAg in a high purity factor VIII concentrate. Higher concentrations of activated protein C (25 micrograms/ml) caused a slight decrease of VIII:CAg, even in the absence of Ca2+ and phospholipids, but did not change VIII:CAg in normal plasma or serum.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, Antigens, Enzyme Activation, Factor VIII, Fibrinolysin, Glycoproteins, Humans, In Vitro Techniques, Protein C, Swine, Urokinase-Type Plasminogen Activator, Journal Article, Research Support, Non-U.S. Gov't
in
Thrombosis Research
volume
31
issue
1
pages
10 pages
publisher
Elsevier
external identifiers
  • Scopus:0020520474
ISSN
0049-3848
DOI
10.1016/0049-3848(83)90006-3
language
English
LU publication?
yes
id
bdd5d302-e062-47cc-80f3-8eee2b691de8
date added to LUP
2016-10-26 12:56:45
date last changed
2016-11-15 09:43:41
@misc{bdd5d302-e062-47cc-80f3-8eee2b691de8,
  abstract     = {<p>The effects of various concentrations of plasmin and activated protein C on the factor VIII procoagulant activity (VIII:C) and coagulant antigen (VIII:CAg) were studied in factor VIII concentrates and normal plasma. Small amounts (0.1 CTA U/ml) of plasmin rapidly destroyed VIII:C, and affected, but did not destroy VIII:CAg, in factor VIII concentrates. In normal plasma larger amounts of plasmin (1.8 CTA U/ml) was required to inactivate VIII:C in order to exceed the neutralizing capacity of alpha 2-antiplasmin. VIII:CAg was unchanged indicating a limited proteolysis. The difference between VIII:C and VIII:CAg was found also in urokinase-activated plasma. Activated protein C (5 micrograms/ml), in the presence of Ca2+ and phospholipids, inactivated VIII:C without affecting VIII:CAg in a high purity factor VIII concentrate. Higher concentrations of activated protein C (25 micrograms/ml) caused a slight decrease of VIII:CAg, even in the absence of Ca2+ and phospholipids, but did not change VIII:CAg in normal plasma or serum.</p>},
  author       = {Holmberg, Lars and Ljung, R and Nilsson, Inga Marie},
  issn         = {0049-3848},
  keyword      = {Animals,Antigens,Enzyme Activation,Factor VIII,Fibrinolysin,Glycoproteins,Humans,In Vitro Techniques,Protein C,Swine,Urokinase-Type Plasminogen Activator,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {07},
  number       = {1},
  pages        = {41--50},
  publisher    = {ARRAY(0xa979198)},
  series       = {Thrombosis Research},
  title        = {The effects of plasmin and protein Ca on factor VIII:C and VIII:CAg},
  url          = {http://dx.doi.org/10.1016/0049-3848(83)90006-3},
  volume       = {31},
  year         = {1983},
}