The effects of plasmin and protein Ca on factor VIII:C and VIII:CAg
(1983) In Thrombosis Research 31(1). p.41-50- Abstract
The effects of various concentrations of plasmin and activated protein C on the factor VIII procoagulant activity (VIII:C) and coagulant antigen (VIII:CAg) were studied in factor VIII concentrates and normal plasma. Small amounts (0.1 CTA U/ml) of plasmin rapidly destroyed VIII:C, and affected, but did not destroy VIII:CAg, in factor VIII concentrates. In normal plasma larger amounts of plasmin (1.8 CTA U/ml) was required to inactivate VIII:C in order to exceed the neutralizing capacity of alpha 2-antiplasmin. VIII:CAg was unchanged indicating a limited proteolysis. The difference between VIII:C and VIII:CAg was found also in urokinase-activated plasma. Activated protein C (5 micrograms/ml), in the presence of Ca2+ and phospholipids,... (More)
The effects of various concentrations of plasmin and activated protein C on the factor VIII procoagulant activity (VIII:C) and coagulant antigen (VIII:CAg) were studied in factor VIII concentrates and normal plasma. Small amounts (0.1 CTA U/ml) of plasmin rapidly destroyed VIII:C, and affected, but did not destroy VIII:CAg, in factor VIII concentrates. In normal plasma larger amounts of plasmin (1.8 CTA U/ml) was required to inactivate VIII:C in order to exceed the neutralizing capacity of alpha 2-antiplasmin. VIII:CAg was unchanged indicating a limited proteolysis. The difference between VIII:C and VIII:CAg was found also in urokinase-activated plasma. Activated protein C (5 micrograms/ml), in the presence of Ca2+ and phospholipids, inactivated VIII:C without affecting VIII:CAg in a high purity factor VIII concentrate. Higher concentrations of activated protein C (25 micrograms/ml) caused a slight decrease of VIII:CAg, even in the absence of Ca2+ and phospholipids, but did not change VIII:CAg in normal plasma or serum.
(Less)
- author
- Holmberg, Lars LU ; Ljung, R LU and Nilsson, Inga Marie
- organization
- publishing date
- 1983-07-01
- type
- Contribution to journal
- publication status
- published
- keywords
- Animals, Antigens, Enzyme Activation, Factor VIII, Fibrinolysin, Glycoproteins, Humans, In Vitro Techniques, Protein C, Swine, Urokinase-Type Plasminogen Activator, Journal Article, Research Support, Non-U.S. Gov't
- in
- Thrombosis Research
- volume
- 31
- issue
- 1
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:6225218
- scopus:0020520474
- ISSN
- 0049-3848
- DOI
- 10.1016/0049-3848(83)90006-3
- language
- English
- LU publication?
- yes
- id
- bdd5d302-e062-47cc-80f3-8eee2b691de8
- date added to LUP
- 2016-10-26 12:56:45
- date last changed
- 2024-01-04 14:56:36
@article{bdd5d302-e062-47cc-80f3-8eee2b691de8, abstract = {{<p>The effects of various concentrations of plasmin and activated protein C on the factor VIII procoagulant activity (VIII:C) and coagulant antigen (VIII:CAg) were studied in factor VIII concentrates and normal plasma. Small amounts (0.1 CTA U/ml) of plasmin rapidly destroyed VIII:C, and affected, but did not destroy VIII:CAg, in factor VIII concentrates. In normal plasma larger amounts of plasmin (1.8 CTA U/ml) was required to inactivate VIII:C in order to exceed the neutralizing capacity of alpha 2-antiplasmin. VIII:CAg was unchanged indicating a limited proteolysis. The difference between VIII:C and VIII:CAg was found also in urokinase-activated plasma. Activated protein C (5 micrograms/ml), in the presence of Ca2+ and phospholipids, inactivated VIII:C without affecting VIII:CAg in a high purity factor VIII concentrate. Higher concentrations of activated protein C (25 micrograms/ml) caused a slight decrease of VIII:CAg, even in the absence of Ca2+ and phospholipids, but did not change VIII:CAg in normal plasma or serum.</p>}}, author = {{Holmberg, Lars and Ljung, R and Nilsson, Inga Marie}}, issn = {{0049-3848}}, keywords = {{Animals; Antigens; Enzyme Activation; Factor VIII; Fibrinolysin; Glycoproteins; Humans; In Vitro Techniques; Protein C; Swine; Urokinase-Type Plasminogen Activator; Journal Article; Research Support, Non-U.S. Gov't}}, language = {{eng}}, month = {{07}}, number = {{1}}, pages = {{41--50}}, publisher = {{Elsevier}}, series = {{Thrombosis Research}}, title = {{The effects of plasmin and protein Ca on factor VIII:C and VIII:CAg}}, url = {{http://dx.doi.org/10.1016/0049-3848(83)90006-3}}, doi = {{10.1016/0049-3848(83)90006-3}}, volume = {{31}}, year = {{1983}}, }