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Proteoglycans of mineralizing rib and epiphyseal cartilage

Lohmander, Stefan LU and Hjerpe, Anders (1975) In Biochimica et Biophysica Acta. General Subjects 404(1). p.93-109
Abstract

Rib cartilage from growing guinea pigs and epiphyseal cartilage from Beagle puppie were separated into three fractions, representing non-mineralized, low mineralized, and high mineralized, tissue, by centrifuging finely ground material in acetone/bromoform density gradients. Following extraction under dissociative conditions, the proteoglycans were fractionated by density gradient ultracentrifugation under associative and dissociative conditions. With the onset of mineralization, the cartilage lost approximately half its content of proteoglycans. The proteoglycans remaining in the calcified cartilage differed in composition and in size from those of nonmineralized tissue. With the increased mineral content of the tissues the ratios of... (More)

Rib cartilage from growing guinea pigs and epiphyseal cartilage from Beagle puppie were separated into three fractions, representing non-mineralized, low mineralized, and high mineralized, tissue, by centrifuging finely ground material in acetone/bromoform density gradients. Following extraction under dissociative conditions, the proteoglycans were fractionated by density gradient ultracentrifugation under associative and dissociative conditions. With the onset of mineralization, the cartilage lost approximately half its content of proteoglycans. The proteoglycans remaining in the calcified cartilage differed in composition and in size from those of nonmineralized tissue. With the increased mineral content of the tissues the ratios of protein to polysaccharide, of chondroitin sulfate to keratan sulfate, and of 4-sulfated to 6-sulfated chondroitin sulfate increased in the proteoglycan fraction. Furthermore, gel chromatograms indicated decreased proportions of very high molecular weight proteoglycans, in mineralized tissue. © 1975.

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publication status
published
subject
in
Biochimica et Biophysica Acta. General Subjects
volume
404
issue
1
pages
17 pages
publisher
Elsevier
external identifiers
  • Scopus:0016819332
ISSN
0304-4165
DOI
10.1016/0304-4165(75)90151-8
language
English
LU publication?
yes
id
cec8fd95-b0e1-43a9-ac42-b7e3406a152f
date added to LUP
2016-05-04 12:41:46
date last changed
2016-12-04 04:51:04
@misc{cec8fd95-b0e1-43a9-ac42-b7e3406a152f,
  abstract     = {<p>Rib cartilage from growing guinea pigs and epiphyseal cartilage from Beagle puppie were separated into three fractions, representing non-mineralized, low mineralized, and high mineralized, tissue, by centrifuging finely ground material in acetone/bromoform density gradients. Following extraction under dissociative conditions, the proteoglycans were fractionated by density gradient ultracentrifugation under associative and dissociative conditions. With the onset of mineralization, the cartilage lost approximately half its content of proteoglycans. The proteoglycans remaining in the calcified cartilage differed in composition and in size from those of nonmineralized tissue. With the increased mineral content of the tissues the ratios of protein to polysaccharide, of chondroitin sulfate to keratan sulfate, and of 4-sulfated to 6-sulfated chondroitin sulfate increased in the proteoglycan fraction. Furthermore, gel chromatograms indicated decreased proportions of very high molecular weight proteoglycans, in mineralized tissue. © 1975.</p>},
  author       = {Lohmander, Stefan and Hjerpe, Anders},
  issn         = {0304-4165},
  language     = {eng},
  month        = {09},
  number       = {1},
  pages        = {93--109},
  publisher    = {ARRAY(0xa894fb8)},
  series       = {Biochimica et Biophysica Acta. General Subjects},
  title        = {Proteoglycans of mineralizing rib and epiphyseal cartilage},
  url          = {http://dx.doi.org/10.1016/0304-4165(75)90151-8},
  volume       = {404},
  year         = {1975},
}