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Adenosine in vertebrate retina : localization, receptor characterization, and function

Blazynski, C and Perez, M T LU (1991) In Cellular and Molecular Neurobiology 11(5). p.463-484
Abstract

1. The uptake of [3H] adenosine into specific populations of cells in the inner retina has been demonstrated. In mammalian retina, the exogenous adenosine that is transported into cells is phosphorylated, thereby maintaining a gradient for transport of the purine into the cell. 2. Endogenous stores of adenosine have been demonstrated by localization of cells that are labeled for adenosine-like immunoreactivity. In the rabbit retina, certain of these cells, the displaced cholinergic, GABAergic amacrine cells, are also labeled for adenosine. 3. Purines are tonically released from dark-adapted rabbit retinas and cultured embryonic chick retinal neurons. Release is significantly increased with K+ and neurotransmitters. The evoked release... (More)

1. The uptake of [3H] adenosine into specific populations of cells in the inner retina has been demonstrated. In mammalian retina, the exogenous adenosine that is transported into cells is phosphorylated, thereby maintaining a gradient for transport of the purine into the cell. 2. Endogenous stores of adenosine have been demonstrated by localization of cells that are labeled for adenosine-like immunoreactivity. In the rabbit retina, certain of these cells, the displaced cholinergic, GABAergic amacrine cells, are also labeled for adenosine. 3. Purines are tonically released from dark-adapted rabbit retinas and cultured embryonic chick retinal neurons. Release is significantly increased with K+ and neurotransmitters. The evoked release consists of adenosine, ATP, and purine metabolites, and while a portion of this release is Ca2+ dependent, one other component may occur via the bidirectional purine nucleoside transporter. 4. Differential distributions of certain enzymes involved in purine metabolism have also been localized to the inner retina. 5. Heterogeneous distributions of the two subtypes of adenosine receptors, A1 and A2, have been demonstrated in the mammalian retina. Coupling of receptors to adenylate cyclase has also been demonstrated. 6. Adenosine A1 receptor agonists significantly inhibit the K(+)-stimulated release of [3H]-acetylcholine from the rabbit retina, suggesting that endogenous adenosine may modulate the light-evoked or tonic release of ACh.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Acetylcholine, Adenosine, Animals, Neurotransmitter Agents, Purines, Receptors, Purinergic, Retina, Vertebrates, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S., Review
in
Cellular and Molecular Neurobiology
volume
11
issue
5
pages
22 pages
publisher
Springer
external identifiers
  • Scopus:0025944542
ISSN
0272-4340
DOI
10.1007/BF00734810
language
English
LU publication?
yes
id
ced5ec31-bd1e-4389-8d4e-1e845e16fff2
date added to LUP
2016-10-27 19:37:10
date last changed
2016-12-04 04:55:00
@misc{ced5ec31-bd1e-4389-8d4e-1e845e16fff2,
  abstract     = {<p>1. The uptake of [3H] adenosine into specific populations of cells in the inner retina has been demonstrated. In mammalian retina, the exogenous adenosine that is transported into cells is phosphorylated, thereby maintaining a gradient for transport of the purine into the cell. 2. Endogenous stores of adenosine have been demonstrated by localization of cells that are labeled for adenosine-like immunoreactivity. In the rabbit retina, certain of these cells, the displaced cholinergic, GABAergic amacrine cells, are also labeled for adenosine. 3. Purines are tonically released from dark-adapted rabbit retinas and cultured embryonic chick retinal neurons. Release is significantly increased with K+ and neurotransmitters. The evoked release consists of adenosine, ATP, and purine metabolites, and while a portion of this release is Ca2+ dependent, one other component may occur via the bidirectional purine nucleoside transporter. 4. Differential distributions of certain enzymes involved in purine metabolism have also been localized to the inner retina. 5. Heterogeneous distributions of the two subtypes of adenosine receptors, A1 and A2, have been demonstrated in the mammalian retina. Coupling of receptors to adenylate cyclase has also been demonstrated. 6. Adenosine A1 receptor agonists significantly inhibit the K(+)-stimulated release of [3H]-acetylcholine from the rabbit retina, suggesting that endogenous adenosine may modulate the light-evoked or tonic release of ACh.</p>},
  author       = {Blazynski, C and Perez, M T},
  issn         = {0272-4340},
  keyword      = {Acetylcholine,Adenosine,Animals,Neurotransmitter Agents,Purines,Receptors, Purinergic,Retina,Vertebrates,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, P.H.S.,Review},
  language     = {eng},
  number       = {5},
  pages        = {463--484},
  publisher    = {ARRAY(0x8e9efe8)},
  series       = {Cellular and Molecular Neurobiology},
  title        = {Adenosine in vertebrate retina : localization, receptor characterization, and function},
  url          = {http://dx.doi.org/10.1007/BF00734810},
  volume       = {11},
  year         = {1991},
}