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The mouthparts enriched odorant binding protein 11 of the Alfalfa Plant Bug Adelphocoris lineolatus displays a preferential binding behavior to host plant secondary metabolites

Sun, Liang; Wei, Yu; Zhang, Dandan LU and Ma, Xiao-Yu (2016) In Frontiers in Physiology 7. p.1-10
Abstract
Odorant binding proteins (OBPs) are proposed to be directly required for odorant discrimination and represent potential interesting targets for pest control. In the notoriously agricultural pest Adelphocoris lineolatus, our previous functional investigation of highly expressed antennal OBPs clearly supported this viewpoint, whereas the findings of the current study by characterizing of AlinOBP11 rather indicated that OBP in hemipterous plant bugs might fulfill a different and tantalizing physiological role. The phylogenetic analysis uncovered that AlinOBP11 together with several homologous bug OBP proteins are potential orthologs, implying they could exhibit a conserved function. Next, the results of expression profiles solidly showed that... (More)
Odorant binding proteins (OBPs) are proposed to be directly required for odorant discrimination and represent potential interesting targets for pest control. In the notoriously agricultural pest Adelphocoris lineolatus, our previous functional investigation of highly expressed antennal OBPs clearly supported this viewpoint, whereas the findings of the current study by characterizing of AlinOBP11 rather indicated that OBP in hemipterous plant bugs might fulfill a different and tantalizing physiological role. The phylogenetic analysis uncovered that AlinOBP11 together with several homologous bug OBP proteins are potential orthologs, implying they could exhibit a conserved function. Next, the results of expression profiles solidly showed that AlinOBP11 was predominantly expressed at adult mouthparts, the most important gustatory organ of Hemiptera mirid bug. Finally, a rigorously selective binding profile was observed in the fluorescence competitive binding assay, in which recombinant AlinOBP11 displayed much stronger binding abilities to non-volatile secondary metabolite compounds than the volatile odorants. These results reflect that AlinOBP11, even its orthologous proteins across bug species, could be associated with a distinctively conserved physiological role such as a crucial carrier for non-volatiles host secondary metabolites in gustatory system.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phylogenetic analysis
in
Frontiers in Physiology
volume
7
pages
10 pages
publisher
Frontiers
external identifiers
  • Scopus:84977635721
ISSN
1664-042X
DOI
10.3389/fphys.2016.00201
language
English
LU publication?
yes
id
d2b2a475-96a1-43ea-bb8e-f581e4fab286
date added to LUP
2016-09-22 12:41:04
date last changed
2016-10-13 05:14:04
@misc{d2b2a475-96a1-43ea-bb8e-f581e4fab286,
  abstract     = {Odorant binding proteins (OBPs) are proposed to be directly required for odorant discrimination and represent potential interesting targets for pest control. In the notoriously agricultural pest Adelphocoris lineolatus, our previous functional investigation of highly expressed antennal OBPs clearly supported this viewpoint, whereas the findings of the current study by characterizing of AlinOBP11 rather indicated that OBP in hemipterous plant bugs might fulfill a different and tantalizing physiological role. The phylogenetic analysis uncovered that AlinOBP11 together with several homologous bug OBP proteins are potential orthologs, implying they could exhibit a conserved function. Next, the results of expression profiles solidly showed that AlinOBP11 was predominantly expressed at adult mouthparts, the most important gustatory organ of Hemiptera mirid bug. Finally, a rigorously selective binding profile was observed in the fluorescence competitive binding assay, in which recombinant AlinOBP11 displayed much stronger binding abilities to non-volatile secondary metabolite compounds than the volatile odorants. These results reflect that AlinOBP11, even its orthologous proteins across bug species, could be associated with a distinctively conserved physiological role such as a crucial carrier for non-volatiles host secondary metabolites in gustatory system.<br/><br/>},
  author       = {Sun, Liang and Wei, Yu and Zhang, Dandan and Ma, Xiao-Yu},
  issn         = {1664-042X},
  keyword      = {phylogenetic analysis},
  language     = {eng},
  pages        = {1--10},
  publisher    = {ARRAY(0x7f92350)},
  series       = {Frontiers in Physiology},
  title        = {The mouthparts enriched odorant binding protein 11 of the Alfalfa Plant Bug Adelphocoris lineolatus displays a preferential binding behavior to host plant secondary metabolites},
  url          = {http://dx.doi.org/10.3389/fphys.2016.00201},
  volume       = {7},
  year         = {2016},
}