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Fluorescence polarisation for immunoreagent characterisation

Önnerfjord, P. LU ; Eremin, S.; Emnéus, J. LU and Marko-Varga, G. LU (1998) In Journal of Immunological Methods 213(1). p.31-39
Abstract

Antibodies were characterised using fluorescence polarisation, a homogeneous assay technique in which all reagents are in solution. Kinetic studies on the association and dissociation of the immunocomplex were performed. A competitive assay was used and the sensitivities, operational linearities, as well as the specificities of the immunoassays were experimentally determined for various antibody preparations with specificity for triazines. Detection limits for atrazine in water samples were determined to be within the range of 0.08-0.4 ng ml-1 using a 5-min incubation time and a 0.5-ml sample volume.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Antibody characterisation, Atrazine, Fluorescence polarisation, Homogenous immunoassay, Triazines
in
Journal of Immunological Methods
volume
213
issue
1
pages
9 pages
publisher
Elsevier
external identifiers
  • Scopus:0031845984
ISSN
0022-1759
DOI
10.1016/S0022-1759(98)00019-2
language
English
LU publication?
yes
id
d953be59-1b7c-42cf-9371-21973892e1de
date added to LUP
2016-10-14 11:37:38
date last changed
2016-11-29 08:39:19
@misc{d953be59-1b7c-42cf-9371-21973892e1de,
  abstract     = {<p>Antibodies were characterised using fluorescence polarisation, a homogeneous assay technique in which all reagents are in solution. Kinetic studies on the association and dissociation of the immunocomplex were performed. A competitive assay was used and the sensitivities, operational linearities, as well as the specificities of the immunoassays were experimentally determined for various antibody preparations with specificity for triazines. Detection limits for atrazine in water samples were determined to be within the range of 0.08-0.4 ng ml<sup>-1</sup> using a 5-min incubation time and a 0.5-ml sample volume.</p>},
  author       = {Önnerfjord, P. and Eremin, S. and Emnéus, J. and Marko-Varga, G.},
  issn         = {0022-1759},
  keyword      = {Antibody characterisation,Atrazine,Fluorescence polarisation,Homogenous immunoassay,Triazines},
  language     = {eng},
  month        = {04},
  number       = {1},
  pages        = {31--39},
  publisher    = {ARRAY(0x9fc7ba0)},
  series       = {Journal of Immunological Methods},
  title        = {Fluorescence polarisation for immunoreagent characterisation},
  url          = {http://dx.doi.org/10.1016/S0022-1759(98)00019-2},
  volume       = {213},
  year         = {1998},
}