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Catechol-O-methyltransferase (COMT) in rat brain : immunoelectron microscopic study with an antiserum against rat recombinant COMT protein

Karhunen, Tuula; Tilgmann, Carola LU ; Ulmanen, Ismo and Panul, Pertti (1995) In Neuroscience Letters 187(1). p.57-60
Abstract

Localization of catechol-O-methyltransferase (COMT) in rat cerebral cortex, neostriatum and cerebellar cortex was studied with pre-embedding immunoelectron microscopy using a specific antiserum raised against rat recombinant COMT protein. In all areas, immunoreactivity was found both in astrocytes and in neuronal processes. Reaction product was seen in the cytoplasm and in association with tubular structures of dendritic processes. Immunoreactivity was also located postsynaptically in dendritic spines and associated with the postsynaptic membrane. Strong immunoreaction was also seen in the cytoplasm of ependymal cells lining the ventricles, and in tanycytes in median eminence. The results suggest that postsynaptic dendritic spines and... (More)

Localization of catechol-O-methyltransferase (COMT) in rat cerebral cortex, neostriatum and cerebellar cortex was studied with pre-embedding immunoelectron microscopy using a specific antiserum raised against rat recombinant COMT protein. In all areas, immunoreactivity was found both in astrocytes and in neuronal processes. Reaction product was seen in the cytoplasm and in association with tubular structures of dendritic processes. Immunoreactivity was also located postsynaptically in dendritic spines and associated with the postsynaptic membrane. Strong immunoreaction was also seen in the cytoplasm of ependymal cells lining the ventricles, and in tanycytes in median eminence. The results suggest that postsynaptic dendritic spines and astrocytic processes may be the sites of catecholamine inactivation by COMT in rat brain. © 1995.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Astrocytes, Catecholamine, Cerebellum, Cortex, Postsynaptic, Striatum
in
Neuroscience Letters
volume
187
issue
1
pages
4 pages
publisher
Elsevier
external identifiers
  • Scopus:0028871197
ISSN
0304-3940
DOI
10.1016/0304-3940(95)11337-V
language
English
LU publication?
no
id
e6908ec7-a682-4911-89bf-6cc255562bb1
date added to LUP
2016-04-11 13:19:05
date last changed
2016-06-29 09:03:34
@misc{e6908ec7-a682-4911-89bf-6cc255562bb1,
  abstract     = {<p>Localization of catechol-O-methyltransferase (COMT) in rat cerebral cortex, neostriatum and cerebellar cortex was studied with pre-embedding immunoelectron microscopy using a specific antiserum raised against rat recombinant COMT protein. In all areas, immunoreactivity was found both in astrocytes and in neuronal processes. Reaction product was seen in the cytoplasm and in association with tubular structures of dendritic processes. Immunoreactivity was also located postsynaptically in dendritic spines and associated with the postsynaptic membrane. Strong immunoreaction was also seen in the cytoplasm of ependymal cells lining the ventricles, and in tanycytes in median eminence. The results suggest that postsynaptic dendritic spines and astrocytic processes may be the sites of catecholamine inactivation by COMT in rat brain. © 1995.</p>},
  author       = {Karhunen, Tuula and Tilgmann, Carola and Ulmanen, Ismo and Panul, Pertti},
  issn         = {0304-3940},
  keyword      = {Astrocytes,Catecholamine,Cerebellum,Cortex,Postsynaptic,Striatum},
  language     = {eng},
  month        = {02},
  number       = {1},
  pages        = {57--60},
  publisher    = {ARRAY(0x9c55958)},
  series       = {Neuroscience Letters},
  title        = {Catechol-O-methyltransferase (COMT) in rat brain : immunoelectron microscopic study with an antiserum against rat recombinant COMT protein},
  url          = {http://dx.doi.org/10.1016/0304-3940(95)11337-V},
  volume       = {187},
  year         = {1995},
}