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Effects of stimulation of the parasympathetic and sympathetic innervations in bursts on the syntheses of polyamines, DNA and protein in salivary glands of the rat;non-adrenergic, non-cholinergic responses : non-adrenergic, non-cholinergic responses

Nilsson, B O LU ; Rosengren, Erik and Ekström, J. (1991) In Acta Physiol.Scand 143(2). p.161-8
Abstract

The increase in the activity of ornithine decarboxylase, the key enzyme in the formation of polyamines, in the sublingual glands of the chloralose-anaesthetized rat in response to stimulation of the parasympathetic innervation was enhanced by employing an intermittent pattern of nerve stimulation. 20 Hz in bursts delivered for 1 second every 10th second over a period of 3 hours increased the activity 1000-fold, whereas a frequency of 2 Hz delivered continuously, and thus giving rise to the same total number of impulses as the burst stimulation, increased the enzyme activity only 50-fold. Already 2 Hz in bursts caused the ornithine decarboxylase activity to increase. A parasympathetic non-adrenergic, non-cholinergic regulation of... (More)

The increase in the activity of ornithine decarboxylase, the key enzyme in the formation of polyamines, in the sublingual glands of the chloralose-anaesthetized rat in response to stimulation of the parasympathetic innervation was enhanced by employing an intermittent pattern of nerve stimulation. 20 Hz in bursts delivered for 1 second every 10th second over a period of 3 hours increased the activity 1000-fold, whereas a frequency of 2 Hz delivered continuously, and thus giving rise to the same total number of impulses as the burst stimulation, increased the enzyme activity only 50-fold. Already 2 Hz in bursts caused the ornithine decarboxylase activity to increase. A parasympathetic non-adrenergic, non-cholinergic regulation of ornithine decarboxylase activity was shown in response to the two modes of stimulation. The increase to 2 and 20 Hz in bursts was 2- and 80-fold, respectively, whereas it was 29-fold to 2 Hz continuously. Increases in the concentration of the polyamine putrescine in response to burst stimulation of the parasympathetic nerve were found both in the absence and in the presence of atropine. The ornithine decarboxylase activity of the submaxillary gland was not affected by the parasympathetic stimulation. Stimulation of the sympathetic innervation was more effective in both types of glands, as to increases in the ornithine decarboxylase activity, when employing the continuous mode. In cultured tissue of the sublingual glands which, in vivo, had been subjected to parasympathetic stimulation in bursts (20 Hz), the syntheses of DNA and protein were increased as judged by the incorporation of radio-labelled thymidine and leucine; in the presence of atropine the stimulation resulted only in increased synthesis of protein.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Animals, Atropine, DNA, Electric Stimulation, Ornithine Decarboxylase, Parasympathetic Nervous System, Polyamines, Protein Biosynthesis, Rats, Rats, Inbred Strains, Salivary Glands, Sympathetic Nervous System
in
Acta Physiol.Scand
volume
143
issue
2
pages
8 pages
publisher
Wiley-Blackwell
external identifiers
  • Scopus:0026059439
ISSN
0001-6772
DOI
10.1111/j.1748-1716.1991.tb09217.x
language
English
LU publication?
yes
id
e9fdb3ca-bbed-4207-9231-a440ebc07182
date added to LUP
2016-06-17 16:47:49
date last changed
2016-07-04 12:59:56
@misc{e9fdb3ca-bbed-4207-9231-a440ebc07182,
  abstract     = {<p>The increase in the activity of ornithine decarboxylase, the key enzyme in the formation of polyamines, in the sublingual glands of the chloralose-anaesthetized rat in response to stimulation of the parasympathetic innervation was enhanced by employing an intermittent pattern of nerve stimulation. 20 Hz in bursts delivered for 1 second every 10th second over a period of 3 hours increased the activity 1000-fold, whereas a frequency of 2 Hz delivered continuously, and thus giving rise to the same total number of impulses as the burst stimulation, increased the enzyme activity only 50-fold. Already 2 Hz in bursts caused the ornithine decarboxylase activity to increase. A parasympathetic non-adrenergic, non-cholinergic regulation of ornithine decarboxylase activity was shown in response to the two modes of stimulation. The increase to 2 and 20 Hz in bursts was 2- and 80-fold, respectively, whereas it was 29-fold to 2 Hz continuously. Increases in the concentration of the polyamine putrescine in response to burst stimulation of the parasympathetic nerve were found both in the absence and in the presence of atropine. The ornithine decarboxylase activity of the submaxillary gland was not affected by the parasympathetic stimulation. Stimulation of the sympathetic innervation was more effective in both types of glands, as to increases in the ornithine decarboxylase activity, when employing the continuous mode. In cultured tissue of the sublingual glands which, in vivo, had been subjected to parasympathetic stimulation in bursts (20 Hz), the syntheses of DNA and protein were increased as judged by the incorporation of radio-labelled thymidine and leucine; in the presence of atropine the stimulation resulted only in increased synthesis of protein.</p>},
  author       = {Nilsson, B O and Rosengren, Erik and Ekström, J.},
  issn         = {0001-6772},
  keyword      = {Animals,Atropine,DNA,Electric Stimulation,Ornithine Decarboxylase,Parasympathetic Nervous System,Polyamines,Protein Biosynthesis,Rats,Rats, Inbred Strains,Salivary Glands,Sympathetic Nervous System},
  language     = {eng},
  number       = {2},
  pages        = {161--8},
  publisher    = {ARRAY(0x7acc6e8)},
  series       = {Acta Physiol.Scand},
  title        = {Effects of stimulation of the parasympathetic and sympathetic innervations in bursts on the syntheses of polyamines, DNA and protein in salivary glands of the rat;non-adrenergic, non-cholinergic responses : non-adrenergic, non-cholinergic responses},
  url          = {http://dx.doi.org/10.1111/j.1748-1716.1991.tb09217.x},
  volume       = {143},
  year         = {1991},
}