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Adaptation to low pH and lignocellulosic inhibitors resulting in ethanolic fermentation and growth of Saccharomyces cerevisiae

Narayanan, Venkatachalam LU ; Sanchez Nogue, Violeta LU ; van Niel, Ed LU and Gorwa-Grauslund, Marie F LU (2016) In AMB Express 6.
Abstract
Lignocellulosic bioethanol from renewable feedstocks using Saccharomyces cerevisiae is a promising alternative to
fossil fuels owing to environmental challenges. S. cerevisiae is frequently challenged by bacterial contamination and
a combination of lignocellulosic inhibitors formed during the pre-treatment, in terms of growth, ethanol yield and
productivity. We investigated the phenotypic robustness of a brewing yeast strain TMB3500 and its ability to adapt to
low pH thereby preventing bacterial contamination along with lignocellulosic inhibitors by short-term adaptation and
adaptive lab evolution (ALE). The short-term adaptation strategy was used to investigate the inherent ability of strain
TMB3500 to activate a... (More)
Lignocellulosic bioethanol from renewable feedstocks using Saccharomyces cerevisiae is a promising alternative to
fossil fuels owing to environmental challenges. S. cerevisiae is frequently challenged by bacterial contamination and
a combination of lignocellulosic inhibitors formed during the pre-treatment, in terms of growth, ethanol yield and
productivity. We investigated the phenotypic robustness of a brewing yeast strain TMB3500 and its ability to adapt to
low pH thereby preventing bacterial contamination along with lignocellulosic inhibitors by short-term adaptation and
adaptive lab evolution (ALE). The short-term adaptation strategy was used to investigate the inherent ability of strain
TMB3500 to activate a robust phenotype involving pre-culturing yeast cells in defined medium with lignocellulosic
inhibitors at pH 5.0 until late exponential phase prior to inoculating them in defined media with the same inhibitor
cocktail at pH 3.7. Adapted cells were able to grow aerobically, ferment anaerobically (glucose exhaustion by 19 ± 5 h
to yield 0.45 ± 0.01 g ethanol g glucose−1) and portray significant detoxification of inhibitors at pH 3.7, when compared
to non-adapted cells. ALE was performed to investigate whether a stable strain could be developed to grow
and ferment at low pH with lignocellulosic inhibitors in a continuous suspension culture. Though a robust population
was obtained after 3600 h with an ability to grow and ferment at pH 3.7 with inhibitors, inhibitor robustness was not
stable as indicated by the characterisation of the evolved culture possibly due to phenotypic plasticity. With further
research, this short-term adaptation and low pH strategy could be successfully applied in lignocellulosic ethanol
plants to prevent bacterial contamination. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Saccharomyce cerevisiae, lignocellulosic inhibitors, phenotypic robustness, adaptation, ethanol yield
in
AMB Express
volume
6
publisher
Springer Open
external identifiers
  • Scopus:84984629805
ISSN
2191-0855
DOI
10.1186/s13568-016-0234-8
language
English
LU publication?
yes
id
ebdd42d6-a99e-4567-a671-f10869e2161d
date added to LUP
2016-08-29 16:43:20
date last changed
2016-10-23 04:45:20
@misc{ebdd42d6-a99e-4567-a671-f10869e2161d,
  abstract     = {Lignocellulosic bioethanol from renewable feedstocks using Saccharomyces cerevisiae is a promising alternative to<br/>fossil fuels owing to environmental challenges. S. cerevisiae is frequently challenged by bacterial contamination and<br/>a combination of lignocellulosic inhibitors formed during the pre-treatment, in terms of growth, ethanol yield and<br/>productivity. We investigated the phenotypic robustness of a brewing yeast strain TMB3500 and its ability to adapt to<br/>low pH thereby preventing bacterial contamination along with lignocellulosic inhibitors by short-term adaptation and<br/>adaptive lab evolution (ALE). The short-term adaptation strategy was used to investigate the inherent ability of strain<br/>TMB3500 to activate a robust phenotype involving pre-culturing yeast cells in defined medium with lignocellulosic<br/>inhibitors at pH 5.0 until late exponential phase prior to inoculating them in defined media with the same inhibitor<br/>cocktail at pH 3.7. Adapted cells were able to grow aerobically, ferment anaerobically (glucose exhaustion by 19 ± 5 h<br/>to yield 0.45 ± 0.01 g ethanol g glucose−1) and portray significant detoxification of inhibitors at pH 3.7, when compared<br/>to non-adapted cells. ALE was performed to investigate whether a stable strain could be developed to grow<br/>and ferment at low pH with lignocellulosic inhibitors in a continuous suspension culture. Though a robust population<br/>was obtained after 3600 h with an ability to grow and ferment at pH 3.7 with inhibitors, inhibitor robustness was not<br/>stable as indicated by the characterisation of the evolved culture possibly due to phenotypic plasticity. With further<br/>research, this short-term adaptation and low pH strategy could be successfully applied in lignocellulosic ethanol<br/>plants to prevent bacterial contamination.},
  author       = {Narayanan, Venkatachalam and Sanchez Nogue, Violeta and van Niel, Ed and Gorwa-Grauslund, Marie F},
  issn         = {2191-0855},
  keyword      = {Saccharomyce cerevisiae,lignocellulosic inhibitors,phenotypic robustness,adaptation,ethanol yield},
  language     = {eng},
  publisher    = {ARRAY(0xae5fca0)},
  series       = {AMB Express},
  title        = {Adaptation to low pH and lignocellulosic inhibitors resulting in ethanolic fermentation and growth of Saccharomyces cerevisiae},
  url          = {http://dx.doi.org/10.1186/s13568-016-0234-8},
  volume       = {6},
  year         = {2016},
}