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Transcriptional deregulation of myc in IgH/myc 6;7 translocation carrying rat immunocytomas

Axelson, H LU ; Pear, W S; Panda, C K; Bazin, H; Klein, G and Sümegi, J (1991) In Genes, Chromosomes and Cancer 3(2). p.142-8
Abstract

We have previously shown that the reciprocal translocation t(6;7) associated with the spontaneous immunocytoma of the Louvain rat (RIC) leads to the juxtaposition of myc to the IgH cluster. In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5' of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc transcription in the RIC system. Run-on analysis showed transcriptional attenuation in the normal rat myc gene, similar to the situation in mice and humans. The attenuation was almost completely abrogated in the three immunocytomas studied. Sequence analysis of two tumors failed to reveal any structural changes within exon... (More)

We have previously shown that the reciprocal translocation t(6;7) associated with the spontaneous immunocytoma of the Louvain rat (RIC) leads to the juxtaposition of myc to the IgH cluster. In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5' of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc transcription in the RIC system. Run-on analysis showed transcriptional attenuation in the normal rat myc gene, similar to the situation in mice and humans. The attenuation was almost completely abrogated in the three immunocytomas studied. Sequence analysis of two tumors failed to reveal any structural changes within exon 1, as found by others in Burkitt's lymphoma. We also show that the transcriptional initiation of myc mRNA is changed in the RICs. In an established line of rat fibroblasts (Rat-2), the more distal myc promoter (P2) is the preferred site of initiation. In RIC, however, only 30% of transcripts were initiated from P2. We found that 40% of the transcripts were initiated from P1 and 30% from a novel promoter, designated P1a, located between P1 and P2.

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author
publishing date
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Contribution to journal
publication status
published
subject
keywords
Animals, Base Sequence, Burkitt Lymphoma, DNA Mutational Analysis, DNA, Neoplasm, Gene Expression Regulation, Neoplastic, Genes, Immunoglobulin, Genes, myc, Humans, Immunoglobulin Heavy Chains, Lymphoma, Mice, Molecular Sequence Data, Plasmacytoma, Promoter Regions, Genetic, Rats, Sequence Homology, Nucleic Acid, Species Specificity, Transcription, Genetic, Translocation, Genetic, Transplantation, Heterologous
in
Genes, Chromosomes and Cancer
volume
3
issue
2
pages
7 pages
publisher
John Wiley & Sons
external identifiers
  • Scopus:0026010649
ISSN
1045-2257
DOI
10.1002/gcc.2870030210
language
English
LU publication?
no
id
fc6d6f86-2d04-4443-be93-cd8d24e3e21b
date added to LUP
2016-08-09 09:20:46
date last changed
2016-08-15 14:17:01
@misc{fc6d6f86-2d04-4443-be93-cd8d24e3e21b,
  abstract     = {<p>We have previously shown that the reciprocal translocation t(6;7) associated with the spontaneous immunocytoma of the Louvain rat (RIC) leads to the juxtaposition of myc to the IgH cluster. In 10 of 14 tumors investigated the breakpoints on the myc carrying chromosome were clustered in a 1.5 kb region 5' of the intact gene, proximal to the myc promoters. In this paper we describe the effect of the translocation on myc transcription in the RIC system. Run-on analysis showed transcriptional attenuation in the normal rat myc gene, similar to the situation in mice and humans. The attenuation was almost completely abrogated in the three immunocytomas studied. Sequence analysis of two tumors failed to reveal any structural changes within exon 1, as found by others in Burkitt's lymphoma. We also show that the transcriptional initiation of myc mRNA is changed in the RICs. In an established line of rat fibroblasts (Rat-2), the more distal myc promoter (P2) is the preferred site of initiation. In RIC, however, only 30% of transcripts were initiated from P2. We found that 40% of the transcripts were initiated from P1 and 30% from a novel promoter, designated P1a, located between P1 and P2.</p>},
  author       = {Axelson, H and Pear, W S and Panda, C K and Bazin, H and Klein, G and Sümegi, J},
  issn         = {1045-2257},
  keyword      = {Animals,Base Sequence,Burkitt Lymphoma,DNA Mutational Analysis,DNA, Neoplasm,Gene Expression Regulation, Neoplastic,Genes, Immunoglobulin,Genes, myc,Humans,Immunoglobulin Heavy Chains,Lymphoma,Mice,Molecular Sequence Data,Plasmacytoma,Promoter Regions, Genetic,Rats,Sequence Homology, Nucleic Acid,Species Specificity,Transcription, Genetic,Translocation, Genetic,Transplantation, Heterologous},
  language     = {eng},
  number       = {2},
  pages        = {142--8},
  publisher    = {ARRAY(0x7a29178)},
  series       = {Genes, Chromosomes and Cancer},
  title        = {Transcriptional deregulation of myc in IgH/myc 6;7 translocation carrying rat immunocytomas},
  url          = {http://dx.doi.org/10.1002/gcc.2870030210},
  volume       = {3},
  year         = {1991},
}