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Effects of receptor agonists on polyamine concentrations and spermidine/spermine N1-acetyltransferase activity in rat parotid gland

Nilsson, B O LU and Rosengren, Elsa (1993) In Experientia 49(10). p.4-881
Abstract

The polyamine putrescine might be formed via a degradation (catalyzed by spermidine/spermine N1-acetyltransferase, SSAT) of the higher polyamines spermidine and spermine to putrescine. The involvement of different intracellular signal pathways in the regulation of putrescine formation was studied in explants and in cultured cells of rat parotid glands by using receptor agonists that activate separate second messenger systems, and measuring their effects on the concentrations of putrescine, spermidine and spermine and on the SSAT activity. The beta-adrenoceptor agonist isoprenaline, which is an activator of cAMP formation, increased the putrescine concentration and stimulated the SSAT activity. Pilocarpine, a drug that activates the... (More)

The polyamine putrescine might be formed via a degradation (catalyzed by spermidine/spermine N1-acetyltransferase, SSAT) of the higher polyamines spermidine and spermine to putrescine. The involvement of different intracellular signal pathways in the regulation of putrescine formation was studied in explants and in cultured cells of rat parotid glands by using receptor agonists that activate separate second messenger systems, and measuring their effects on the concentrations of putrescine, spermidine and spermine and on the SSAT activity. The beta-adrenoceptor agonist isoprenaline, which is an activator of cAMP formation, increased the putrescine concentration and stimulated the SSAT activity. Pilocarpine, a drug that activates the muscarinic receptors and thereby enhances the phosphoinositide turnover, had no effect on either the polyamine concentrations or on the SSAT activity. Epidermal growth factor (EGF), which induces activation of a protein tyrosine kinase, had no effect on the polyamine concentrations or on the SSAT activity. The adenylate cyclase activator forskolin increased the glandular levels of putrescine. Taken together, these findings suggest that increases in putrescine concentration in cultured rat parotid gland cells are accompanied by accumulation of cAMP.

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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Acetyltransferases, Animals, Cells, Cultured, Colforsin, Cyclic AMP, Epidermal Growth Factor, Female, Isoproterenol, Male, Parotid Gland, Pilocarpine, Putrescine, Rats, Signal Transduction
in
Experientia
volume
49
issue
10
pages
4 pages
publisher
Springer
external identifiers
  • Scopus:0027366001
ISSN
0014-4754
DOI
10.1007/BF01952602
language
English
LU publication?
yes
id
ff5bb28d-527a-4622-a231-588e8462afff
date added to LUP
2016-06-17 16:46:12
date last changed
2016-06-28 12:03:45
@misc{ff5bb28d-527a-4622-a231-588e8462afff,
  abstract     = {<p>The polyamine putrescine might be formed via a degradation (catalyzed by spermidine/spermine N1-acetyltransferase, SSAT) of the higher polyamines spermidine and spermine to putrescine. The involvement of different intracellular signal pathways in the regulation of putrescine formation was studied in explants and in cultured cells of rat parotid glands by using receptor agonists that activate separate second messenger systems, and measuring their effects on the concentrations of putrescine, spermidine and spermine and on the SSAT activity. The beta-adrenoceptor agonist isoprenaline, which is an activator of cAMP formation, increased the putrescine concentration and stimulated the SSAT activity. Pilocarpine, a drug that activates the muscarinic receptors and thereby enhances the phosphoinositide turnover, had no effect on either the polyamine concentrations or on the SSAT activity. Epidermal growth factor (EGF), which induces activation of a protein tyrosine kinase, had no effect on the polyamine concentrations or on the SSAT activity. The adenylate cyclase activator forskolin increased the glandular levels of putrescine. Taken together, these findings suggest that increases in putrescine concentration in cultured rat parotid gland cells are accompanied by accumulation of cAMP.</p>},
  author       = {Nilsson, B O and Rosengren, Elsa},
  issn         = {0014-4754},
  keyword      = {Acetyltransferases,Animals,Cells, Cultured,Colforsin,Cyclic AMP,Epidermal Growth Factor,Female,Isoproterenol,Male,Parotid Gland,Pilocarpine,Putrescine,Rats,Signal Transduction},
  language     = {eng},
  month        = {10},
  number       = {10},
  pages        = {4--881},
  publisher    = {ARRAY(0x8597aa0)},
  series       = {Experientia},
  title        = {Effects of receptor agonists on polyamine concentrations and spermidine/spermine N1-acetyltransferase activity in rat parotid gland},
  url          = {http://dx.doi.org/10.1007/BF01952602},
  volume       = {49},
  year         = {1993},
}