Modeling of Enzymatic Reaction for Pharmaceutical Production
(2024) KETM05 20241Chemical Engineering (M.Sc.Eng.)
- Abstract (Swedish)
- By modeling of an enzymatic reaction for pharmaceutical production it is possible to get a better understand the mechanism of the enzymatic reaction, and to find an optimal condition for the process step. The enzyme in question is enteropeptidase that cleaves proteins by hy-drolysis. Enteropeptidase can cleave at many different cleaving sites, if the amino acid Argi-nine or Lysine is at the first position (P1) and no other basic amino acid is present at the posi-tion close to it. The protein investigated have two cleaving sites where one of them is wanted and the other one is not wanted. By collecting data for different temperature and enzyme concentrations, a model has been set up in Python. A Michaelis–Menten-kinetic has been used as a... (More)
- By modeling of an enzymatic reaction for pharmaceutical production it is possible to get a better understand the mechanism of the enzymatic reaction, and to find an optimal condition for the process step. The enzyme in question is enteropeptidase that cleaves proteins by hy-drolysis. Enteropeptidase can cleave at many different cleaving sites, if the amino acid Argi-nine or Lysine is at the first position (P1) and no other basic amino acid is present at the posi-tion close to it. The protein investigated have two cleaving sites where one of them is wanted and the other one is not wanted. By collecting data for different temperature and enzyme concentrations, a model has been set up in Python. A Michaelis–Menten-kinetic has been used as a starting point for the reaction kinetics. To find the correlation with temperature Arrhenius plot weas used and it gave a good fit. The reaction rate coefficients was found for temperature between 5 and 30℃. From the model different scenarios can be set up, and the selectivity is better at low temperature. Validation also showed that the model is a good presentation of the real world even if it has some assumption such as no backwards reaction for production of the enzyme complex. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9169650
- author
- Wessman, Alva LU
- supervisor
- organization
- course
- KETM05 20241
- year
- 2024
- type
- H2 - Master's Degree (Two Years)
- subject
- language
- English
- id
- 9169650
- date added to LUP
- 2024-08-05 07:46:24
- date last changed
- 2024-08-05 07:46:27
@misc{9169650,
abstract = {{By modeling of an enzymatic reaction for pharmaceutical production it is possible to get a better understand the mechanism of the enzymatic reaction, and to find an optimal condition for the process step. The enzyme in question is enteropeptidase that cleaves proteins by hy-drolysis. Enteropeptidase can cleave at many different cleaving sites, if the amino acid Argi-nine or Lysine is at the first position (P1) and no other basic amino acid is present at the posi-tion close to it. The protein investigated have two cleaving sites where one of them is wanted and the other one is not wanted. By collecting data for different temperature and enzyme concentrations, a model has been set up in Python. A Michaelis–Menten-kinetic has been used as a starting point for the reaction kinetics. To find the correlation with temperature Arrhenius plot weas used and it gave a good fit. The reaction rate coefficients was found for temperature between 5 and 30℃. From the model different scenarios can be set up, and the selectivity is better at low temperature. Validation also showed that the model is a good presentation of the real world even if it has some assumption such as no backwards reaction for production of the enzyme complex.}},
author = {{Wessman, Alva}},
language = {{eng}},
note = {{Student Paper}},
title = {{Modeling of Enzymatic Reaction for Pharmaceutical Production}},
year = {{2024}},
}