A Microarray platform for "omics" analysis
(2001) In SPIE proceedings series 4414. p.157-163- Abstract
- Microarray technology has revolutionized genetic analysis. However, limitations in genome analysis has lead to renewed interest in establishing 'omic' strategies. As we enter the post-genomic era, new microarray technologies are needed to address these new classes of 'omic' targets, such as proteins, as well as lipids and carbohydrates. We have developed a microarray platform that combines self- assembling monolayers with the biotin-streptavidin system to provide a robust, versatile immobilization scheme. A hydrophobic film is patterned on the surface creating an array of tension wells that eliminates evaporation effects thereby reducing the shear stress to which biomolecules are exposed to during immobilization. The streptavidin linker... (More)
- Microarray technology has revolutionized genetic analysis. However, limitations in genome analysis has lead to renewed interest in establishing 'omic' strategies. As we enter the post-genomic era, new microarray technologies are needed to address these new classes of 'omic' targets, such as proteins, as well as lipids and carbohydrates. We have developed a microarray platform that combines self- assembling monolayers with the biotin-streptavidin system to provide a robust, versatile immobilization scheme. A hydrophobic film is patterned on the surface creating an array of tension wells that eliminates evaporation effects thereby reducing the shear stress to which biomolecules are exposed to during immobilization. The streptavidin linker layer makes it possible to adapt and/or develop microarray based assays using virtually any class of biomolecules including: carbohydrates, peptides, antibodies, receptors, as well as them ore traditional DNA based arrays. Our microarray technology is designed to furnish seamless compatibility across the various 'omic' platforms by providing a common blueprint for fabricating and analyzing arrays. The prototype microarray uses a microscope slide footprint patterned with 2 by 96 flat wells. Data on the microarray platform will be presented. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/004f7496-1941-497d-a67a-6f59fea48c3d
- author
- Mecklenburg, Michael and Xie, Bin LU
- organization
- publishing date
- 2001
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- International Conference on Sensor Technology (ISTC 2001)
- series title
- SPIE proceedings series
- editor
- Zhou, Yikai and Xu, Shunqing
- volume
- 4414
- pages
- 7 pages
- publisher
- SPIE
- external identifiers
-
- scopus:0035759762
- ISSN
- 0277-786X
- ISBN
- 0819441198
- 9780819441195
- DOI
- 10.1117/12.440149
- language
- English
- LU publication?
- yes
- id
- 004f7496-1941-497d-a67a-6f59fea48c3d
- date added to LUP
- 2021-10-27 21:34:55
- date last changed
- 2022-02-02 00:55:13
@inproceedings{004f7496-1941-497d-a67a-6f59fea48c3d,
abstract = {{Microarray technology has revolutionized genetic analysis. However, limitations in genome analysis has lead to renewed interest in establishing 'omic' strategies. As we enter the post-genomic era, new microarray technologies are needed to address these new classes of 'omic' targets, such as proteins, as well as lipids and carbohydrates. We have developed a microarray platform that combines self- assembling monolayers with the biotin-streptavidin system to provide a robust, versatile immobilization scheme. A hydrophobic film is patterned on the surface creating an array of tension wells that eliminates evaporation effects thereby reducing the shear stress to which biomolecules are exposed to during immobilization. The streptavidin linker layer makes it possible to adapt and/or develop microarray based assays using virtually any class of biomolecules including: carbohydrates, peptides, antibodies, receptors, as well as them ore traditional DNA based arrays. Our microarray technology is designed to furnish seamless compatibility across the various 'omic' platforms by providing a common blueprint for fabricating and analyzing arrays. The prototype microarray uses a microscope slide footprint patterned with 2 by 96 flat wells. Data on the microarray platform will be presented.}},
author = {{Mecklenburg, Michael and Xie, Bin}},
booktitle = {{International Conference on Sensor Technology (ISTC 2001)}},
editor = {{Zhou, Yikai and Xu, Shunqing}},
isbn = {{0819441198}},
issn = {{0277-786X}},
language = {{eng}},
pages = {{157--163}},
publisher = {{SPIE}},
series = {{SPIE proceedings series}},
title = {{A Microarray platform for "omics" analysis}},
url = {{http://dx.doi.org/10.1117/12.440149}},
doi = {{10.1117/12.440149}},
volume = {{4414}},
year = {{2001}},
}