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In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays

Burmeister, Jason J.; Pomerleau, Francois; Quintero, Jorge E.; Huettl, Peter; Ai, Yi; Jakobsson, Johan LU ; Lundblad, Martin LU ; Heuer, Andreas LU ; Slevin, John T and Gerhardt, Greg A. (2018) In Neuromethods 130. p.327-351
Abstract

Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is... (More)

Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is thoroughly explained with special attention paid to MEA enzyme coating and cleaning for the benefit of other investigators. These data support that optogenetic control of glutamate signaling is robust with certain advantages as compared to other methods to modulate the in vivo control of glutamate signaling.

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author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Amperometry, Array, Biosensor, Electrochemistry, Glutamate, Glutamate oxidase, Microelectrode, Neurotransmitter, Optogenetics
in
Neuromethods
volume
130
pages
25 pages
publisher
Humana Press
external identifiers
  • scopus:85030130464
ISSN
08932336
19406045
DOI
language
English
LU publication?
yes
id
005b95af-8e0c-48b6-8d9d-26987591c9a8
date added to LUP
2017-10-24 10:05:29
date last changed
2018-05-29 11:03:48
@inbook{005b95af-8e0c-48b6-8d9d-26987591c9a8,
  abstract     = {<p>Direct electrochemical measurements of glutamate release in vivo were combined with optogenetics in order to examine light-induced control of glutamate neurotransmission in the rodent brain. Self-referenced recordings of glutamate using ceramic-based microelectrode arrays (MEAs) in hippocampus and frontal cortex demonstrated precise optical control of light-induced glutamate release through channelrhodopsin (ChR2) expression in both rat hippocampus and frontal cortex. Although the virus was only injected unilaterally, bilateral and rostro-caudal expression was observed in slice imaging, indicating diffusion and active transport of the viral particles. Methodology for the optogenetic control of glutamate signaling in the rat brain is thoroughly explained with special attention paid to MEA enzyme coating and cleaning for the benefit of other investigators. These data support that optogenetic control of glutamate signaling is robust with certain advantages as compared to other methods to modulate the in vivo control of glutamate signaling.</p>},
  author       = {Burmeister, Jason J. and Pomerleau, Francois and Quintero, Jorge E. and Huettl, Peter and Ai, Yi and Jakobsson, Johan and Lundblad, Martin and Heuer, Andreas and Slevin, John T and Gerhardt, Greg A.},
  issn         = {08932336},
  keyword      = {Amperometry,Array,Biosensor,Electrochemistry,Glutamate,Glutamate oxidase,Microelectrode,Neurotransmitter,Optogenetics},
  language     = {eng},
  pages        = {327--351},
  publisher    = {Humana Press},
  series       = {Neuromethods},
  title        = {In vivo electrochemical studies of optogenetic control of glutamate signaling measured using enzyme-based ceramic microelectrode arrays},
  url          = {http://dx.doi.org/},
  volume       = {130},
  year         = {2018},
}