A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography
Pierantoni, Maria LU
; Silva Barreto, Isabella
LU
; Hammerman, Malin
LU
; Verhoeven, Lissa
LU
; Törnquist, Elin
LU
; Novak, Vladimir
; Mokso, Rajmund
LU
; Eliasson, Pernilla
and Isaksson, Hanna
LU
(2021)
In Scientific Reports
11(1).
- Abstract
Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes).... (More)
Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.
(Less)
- author
- Pierantoni, Maria
LU
; Silva Barreto, Isabella
LU
; Hammerman, Malin
LU
; Verhoeven, Lissa
LU
; Törnquist, Elin
LU
; Novak, Vladimir
; Mokso, Rajmund
LU
; Eliasson, Pernilla
and Isaksson, Hanna
LU
- organization
- publishing date
- 2021-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 11
- issue
- 1
- article number
- 17313
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:34453067
- scopus:85113748101
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-021-96589-w
- language
- English
- LU publication?
- yes
- id
- 0088d9b2-5526-448b-956c-dfa5726c9d03
- date added to LUP
- 2021-09-17 11:33:04
- date last changed
- 2024-09-08 23:29:03
@article{0088d9b2-5526-448b-956c-dfa5726c9d03,
abstract = {{<p>Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.</p>}},
author = {{Pierantoni, Maria and Silva Barreto, Isabella and Hammerman, Malin and Verhoeven, Lissa and Törnquist, Elin and Novak, Vladimir and Mokso, Rajmund and Eliasson, Pernilla and Isaksson, Hanna}},
issn = {{2045-2322}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography}},
url = {{http://dx.doi.org/10.1038/s41598-021-96589-w}},
doi = {{10.1038/s41598-021-96589-w}},
volume = {{11}},
year = {{2021}},
}