Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography

Pierantoni, Maria LU ; Silva Barreto, Isabella LU ; Hammerman, Malin LU ; Verhoeven, Lissa LU ; Törnquist, Elin LU ; Novak, Vladimir ; Mokso, Rajmund LU ; Eliasson, Pernilla and Isaksson, Hanna LU (2021) In Scientific Reports 11(1).
Abstract

Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes).... (More)

Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
11
issue
1
article number
17313
publisher
Nature Publishing Group
external identifiers
  • pmid:34453067
  • scopus:85113748101
ISSN
2045-2322
DOI
10.1038/s41598-021-96589-w
language
English
LU publication?
yes
id
0088d9b2-5526-448b-956c-dfa5726c9d03
date added to LUP
2021-09-17 11:33:04
date last changed
2022-08-12 00:00:04
@article{0088d9b2-5526-448b-956c-dfa5726c9d03,
  abstract     = {{<p>Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part the result of the mechanical stimulation conveyed by the muscles. To fully understand how their microstructure responds to mechanical loading a non-invasive approach for 3D high resolution imaging suitable for soft tissue is required. Here we propose a protocol that can capture the complex 3D organization of the Achilles tendon microstructure, using phase-contrast enhanced synchrotron micro-tomography (SR-PhC-μCT). We investigate the effects that sample preparation and imaging conditions have on the resulting image quality, by considering four types of sample preparations and two imaging setups (sub-micrometric and micrometric final pixel sizes). The image quality is assessed using four quantitative parameters. The results show that for studying tendon collagen fibers, conventional invasive sample preparations such as fixation and embedding are not necessary or advantageous. Instead, fresh frozen samples result in high-quality images that capture the complex 3D organization of tendon fibers in conditions as close as possible to natural. The comprehensive nature of this innovative study by SR-PhC-μCT breaks ground for future studies of soft complex biological tissue in 3D with high resolution in close to natural conditions, which could be further used for in situ characterization of how soft tissue responds to mechanical stimuli on a microscopic level.</p>}},
  author       = {{Pierantoni, Maria and Silva Barreto, Isabella and Hammerman, Malin and Verhoeven, Lissa and Törnquist, Elin and Novak, Vladimir and Mokso, Rajmund and Eliasson, Pernilla and Isaksson, Hanna}},
  issn         = {{2045-2322}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Scientific Reports}},
  title        = {{A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography}},
  url          = {{http://dx.doi.org/10.1038/s41598-021-96589-w}},
  doi          = {{10.1038/s41598-021-96589-w}},
  volume       = {{11}},
  year         = {{2021}},
}