Development of wide-pore CLEAR supports for applications involving biological macromolecules.
(2001) In Polymer Bulletin 46(5). p.381-387- Abstract
- The CLEAR supports were originally developed for solid-phase synthesis. In this study, two wide-pore versions of the resins were prepared by radical suspension polymerization using toluene as the porogen. Trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate was used as the cross-linking agent. Resins of two different porosities were synthesized. The permeability to biological macromolecules was investigated by size exclusion chromatography, using the resin beads as the stationary phase. One of the resins was able to retard thyroglobulin (669 kDa) while the other, prepared with a lower amount of porogen, showed a lower fractionation range (1-60 kDa).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1122670
- author
- Cederfur, Josefine LU and Kempe, Maria LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Polymer Bulletin
- volume
- 46
- issue
- 5
- pages
- 381 - 387
- publisher
- Springer
- external identifiers
-
- scopus:0034881044
- ISSN
- 0170-0839
- DOI
- 10.1007/s002890170046
- language
- English
- LU publication?
- yes
- id
- 009387dd-f252-4c7b-a86e-659d1b190797 (old id 1122670)
- date added to LUP
- 2016-04-01 11:34:19
- date last changed
- 2022-01-26 07:09:18
@article{009387dd-f252-4c7b-a86e-659d1b190797, abstract = {{The CLEAR supports were originally developed for solid-phase synthesis. In this study, two wide-pore versions of the resins were prepared by radical suspension polymerization using toluene as the porogen. Trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate was used as the cross-linking agent. Resins of two different porosities were synthesized. The permeability to biological macromolecules was investigated by size exclusion chromatography, using the resin beads as the stationary phase. One of the resins was able to retard thyroglobulin (669 kDa) while the other, prepared with a lower amount of porogen, showed a lower fractionation range (1-60 kDa).}}, author = {{Cederfur, Josefine and Kempe, Maria}}, issn = {{0170-0839}}, language = {{eng}}, number = {{5}}, pages = {{381--387}}, publisher = {{Springer}}, series = {{Polymer Bulletin}}, title = {{Development of wide-pore CLEAR supports for applications involving biological macromolecules.}}, url = {{http://dx.doi.org/10.1007/s002890170046}}, doi = {{10.1007/s002890170046}}, volume = {{46}}, year = {{2001}}, }