Advanced

Strain-dependent variance in short-term adaptation effects of two xylose-fermenting strains of Saccharomyces cerevisiae

van Dijk, Marlous ; Erdei, Borbála LU ; Galbe, Mats LU ; Nygård, Yvonne and Olsson, Lisbeth (2019) In Bioresource Technology 292.
Abstract

The limited tolerance of Saccharomyces cerevisiae to the inhibitors present in lignocellulosic hydrolysates is a major challenge in second-generation bioethanol production. Short-term adaptation of the yeast to lignocellulosic hydrolysates during cell propagation has been shown to improve its tolerance, and thus its performance in lignocellulose fermentation. The aim of this study was to investigate the short-term adaptation effects in yeast strains with different genetic backgrounds. Fed-batch propagation cultures were supplemented with 40% wheat straw hydrolysate during the feed phase to adapt two different pentose-fermenting strains, CR01 and KE6-12. The harvested cells were used to inoculate fermentation media containing 80% or 90%... (More)

The limited tolerance of Saccharomyces cerevisiae to the inhibitors present in lignocellulosic hydrolysates is a major challenge in second-generation bioethanol production. Short-term adaptation of the yeast to lignocellulosic hydrolysates during cell propagation has been shown to improve its tolerance, and thus its performance in lignocellulose fermentation. The aim of this study was to investigate the short-term adaptation effects in yeast strains with different genetic backgrounds. Fed-batch propagation cultures were supplemented with 40% wheat straw hydrolysate during the feed phase to adapt two different pentose-fermenting strains, CR01 and KE6-12. The harvested cells were used to inoculate fermentation media containing 80% or 90% wheat straw hydrolysate. The specific ethanol productivity during fermentation was up to 3.6 times higher for CR01 and 1.6 times higher for KE6-12 following adaptation. The influence of physiological parameters such as viability, storage carbohydrate content, and metabolite yields following short-term adaptation demonstrated that short-term adaptation was strain dependent.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ethanol, Industrial Saccharomyces cerevisiae strains, Inhibition, Short-term adaptation, Wheat straw hydrolysate
in
Bioresource Technology
volume
292
article number
121922
publisher
Elsevier
external identifiers
  • scopus:85071998550
  • pmid:31398543
ISSN
0960-8524
DOI
10.1016/j.biortech.2019.121922
language
English
LU publication?
yes
id
00ac23bf-a7f4-470a-a013-64390bc04071
date added to LUP
2019-09-16 12:03:40
date last changed
2020-05-24 06:25:41
@article{00ac23bf-a7f4-470a-a013-64390bc04071,
  abstract     = {<p>The limited tolerance of Saccharomyces cerevisiae to the inhibitors present in lignocellulosic hydrolysates is a major challenge in second-generation bioethanol production. Short-term adaptation of the yeast to lignocellulosic hydrolysates during cell propagation has been shown to improve its tolerance, and thus its performance in lignocellulose fermentation. The aim of this study was to investigate the short-term adaptation effects in yeast strains with different genetic backgrounds. Fed-batch propagation cultures were supplemented with 40% wheat straw hydrolysate during the feed phase to adapt two different pentose-fermenting strains, CR01 and KE6-12. The harvested cells were used to inoculate fermentation media containing 80% or 90% wheat straw hydrolysate. The specific ethanol productivity during fermentation was up to 3.6 times higher for CR01 and 1.6 times higher for KE6-12 following adaptation. The influence of physiological parameters such as viability, storage carbohydrate content, and metabolite yields following short-term adaptation demonstrated that short-term adaptation was strain dependent.</p>},
  author       = {van Dijk, Marlous and Erdei, Borbála and Galbe, Mats and Nygård, Yvonne and Olsson, Lisbeth},
  issn         = {0960-8524},
  language     = {eng},
  publisher    = {Elsevier},
  series       = {Bioresource Technology},
  title        = {Strain-dependent variance in short-term adaptation effects of two xylose-fermenting strains of Saccharomyces cerevisiae},
  url          = {http://dx.doi.org/10.1016/j.biortech.2019.121922},
  doi          = {10.1016/j.biortech.2019.121922},
  volume       = {292},
  year         = {2019},
}