Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Microcolumn chromatofocusing using slurry-packed Mono P anion-exchanger beads as chromatographic matrix

Palm, A ; Hakansson, L and Marko-Varga, György LU (2003) In Chromatographia 58(11-12). p.707-712
Abstract
Chromatofocusing of proteins in a microcolumn format (750 mum i.d.) was evaluated with slurry-packed Mono P anion-exchanger beads (10 pin bead size) as chromatographic matrix. Evaluation was performed with three standard proteins in terms of flow rate, Polybuffer 96-acetic acid, pH 6.0, concentration (eluent buffer strength), and detection limit. A column with the dimension 750 pin i.d. x 50 mm length, a flow rate of 20 muL min(-1),and a 1:10-1:20 Polybuffer 96-acetic acid aqueous dilution showed the best resolution between the proteins. Detection limits were 3 mug mL(-1) for carbonic anhydrase 1 and 6 mug mL(-1) for myoglobin A employing UV-defection at 280 nm.
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chromatographia
volume
58
issue
11-12
pages
707 - 712
publisher
Vieweg Verlag
external identifiers
  • wos:000224674500005
  • scopus:0346690016
ISSN
0009-5893
DOI
10.1365/s10337-003-0104-9
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
id
00d11dd2-5989-4c03-b101-d0abf3b29b48 (old id 138354)
date added to LUP
2016-04-01 15:58:10
date last changed
2022-04-22 18:43:12
@article{00d11dd2-5989-4c03-b101-d0abf3b29b48,
  abstract     = {{Chromatofocusing of proteins in a microcolumn format (750 mum i.d.) was evaluated with slurry-packed Mono P anion-exchanger beads (10 pin bead size) as chromatographic matrix. Evaluation was performed with three standard proteins in terms of flow rate, Polybuffer 96-acetic acid, pH 6.0, concentration (eluent buffer strength), and detection limit. A column with the dimension 750 pin i.d. x 50 mm length, a flow rate of 20 muL min(-1),and a 1:10-1:20 Polybuffer 96-acetic acid aqueous dilution showed the best resolution between the proteins. Detection limits were 3 mug mL(-1) for carbonic anhydrase 1 and 6 mug mL(-1) for myoglobin A employing UV-defection at 280 nm.}},
  author       = {{Palm, A and Hakansson, L and Marko-Varga, György}},
  issn         = {{0009-5893}},
  language     = {{eng}},
  number       = {{11-12}},
  pages        = {{707--712}},
  publisher    = {{Vieweg Verlag}},
  series       = {{Chromatographia}},
  title        = {{Microcolumn chromatofocusing using slurry-packed Mono P anion-exchanger beads as chromatographic matrix}},
  url          = {{http://dx.doi.org/10.1365/s10337-003-0104-9}},
  doi          = {{10.1365/s10337-003-0104-9}},
  volume       = {{58}},
  year         = {{2003}},
}