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Differences in acoustic impedance of fresh and embedded human trabecular bone samples - Scanning acoustic microscopy and numerical evaluation

Ojanen, Xiaowei; Töyräs, Juha; Inkinen, Satu I.; Malo, Markus K H; Isaksson, Hanna LU and Jurvelin, Jukka S. (2016) In Journal of the Acoustical Society of America 140(3). p.1931-1936
Abstract

Trabecular bone samples are traditionally embedded and polished for scanning acoustic microscopy (SAM). The effect of sample processing, including dehydration, on the acoustic impedance of bone is unknown. In this study, acoustic impedance of human trabecular bone samples (n = 8) was experimentally assessed before (fresh) and after embedding using SAM and two-dimensional (2-D) finite-difference time domain simulations. Fresh samples were polished with sandpapers of different grit (P1000, P2500, and P4000). Experimental results indicated that acoustic impedance of samples increased significantly after embedding [mean values 3.7 MRayl (fresh), 6.1 MRayl (embedded), p < 0.001]. After polishing with different papers, no significant... (More)

Trabecular bone samples are traditionally embedded and polished for scanning acoustic microscopy (SAM). The effect of sample processing, including dehydration, on the acoustic impedance of bone is unknown. In this study, acoustic impedance of human trabecular bone samples (n = 8) was experimentally assessed before (fresh) and after embedding using SAM and two-dimensional (2-D) finite-difference time domain simulations. Fresh samples were polished with sandpapers of different grit (P1000, P2500, and P4000). Experimental results indicated that acoustic impedance of samples increased significantly after embedding [mean values 3.7 MRayl (fresh), 6.1 MRayl (embedded), p < 0.001]. After polishing with different papers, no significant changes in acoustic impedance were found, even though higher mean values were detected after polishing with finer (P2500 and P4000) papers. A linear correlation (r = 0.854, p < 0.05) was found between the acoustic impedance values of embedded and fresh bone samples polished using P2500 SiC paper. In numerical simulations dehydration increased the acoustic impedance of trabecular bone (38%), whereas changes in surface roughness of bone had a minor effect on the acoustic impedance (-1.56%/0.1 μm). Thereby, the numerical simulations corroborated the experimental findings. In conclusion, acoustic impedance measurement of fresh trabecular bone is possible and may provide realistic material values similar to those of living bone.

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organization
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type
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publication status
published
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in
Journal of the Acoustical Society of America
volume
140
issue
3
pages
6 pages
publisher
American Institute of Physics
external identifiers
  • scopus:84988688660
  • wos:000386932500052
ISSN
0001-4966
DOI
10.1121/1.4962347
language
English
LU publication?
yes
id
01331cff-08b2-40e8-a078-cbb953003038
date added to LUP
2016-11-23 11:54:20
date last changed
2017-08-27 06:29:42
@article{01331cff-08b2-40e8-a078-cbb953003038,
  abstract     = {<p>Trabecular bone samples are traditionally embedded and polished for scanning acoustic microscopy (SAM). The effect of sample processing, including dehydration, on the acoustic impedance of bone is unknown. In this study, acoustic impedance of human trabecular bone samples (n = 8) was experimentally assessed before (fresh) and after embedding using SAM and two-dimensional (2-D) finite-difference time domain simulations. Fresh samples were polished with sandpapers of different grit (P1000, P2500, and P4000). Experimental results indicated that acoustic impedance of samples increased significantly after embedding [mean values 3.7 MRayl (fresh), 6.1 MRayl (embedded), p &lt; 0.001]. After polishing with different papers, no significant changes in acoustic impedance were found, even though higher mean values were detected after polishing with finer (P2500 and P4000) papers. A linear correlation (r = 0.854, p &lt; 0.05) was found between the acoustic impedance values of embedded and fresh bone samples polished using P2500 SiC paper. In numerical simulations dehydration increased the acoustic impedance of trabecular bone (38%), whereas changes in surface roughness of bone had a minor effect on the acoustic impedance (-1.56%/0.1 μm). Thereby, the numerical simulations corroborated the experimental findings. In conclusion, acoustic impedance measurement of fresh trabecular bone is possible and may provide realistic material values similar to those of living bone.</p>},
  author       = {Ojanen, Xiaowei and Töyräs, Juha and Inkinen, Satu I. and Malo, Markus K H and Isaksson, Hanna and Jurvelin, Jukka S.},
  issn         = {0001-4966},
  language     = {eng},
  month        = {09},
  number       = {3},
  pages        = {1931--1936},
  publisher    = {American Institute of Physics},
  series       = {Journal of the Acoustical Society of America},
  title        = {Differences in acoustic impedance of fresh and embedded human trabecular bone samples - Scanning acoustic microscopy and numerical evaluation},
  url          = {http://dx.doi.org/10.1121/1.4962347},
  volume       = {140},
  year         = {2016},
}