Tracking actomyosin at fluorescence check points.
Lard, Mercy LU ; Siethoff, Lasse Ten ; Månsson, Alf and Linke, Heiner LU
(2013)
In Scientific Reports
3.
- Abstract
- Emerging concepts for on-chip biotechnologies aim to replace microfluidic flow by active, molecular-motor driven transport of cytoskeletal filaments, including applications in bio-simulation, biocomputation, diagnostics, and drug screening. Many of these applications require reliable detection, with minimal data acquisition, of filaments at many, local checkpoints in a device consisting of a potentially complex network of channels that guide filament motion. Here we develop such a detection system using actomyosin motility. Detection points consist of pairs of gold lines running perpendicular to nanochannels that guide motion of fluorescent actin filaments. Fluorescence interference contrast (FLIC) is used to locally enhance the signal at... (More)
- Emerging concepts for on-chip biotechnologies aim to replace microfluidic flow by active, molecular-motor driven transport of cytoskeletal filaments, including applications in bio-simulation, biocomputation, diagnostics, and drug screening. Many of these applications require reliable detection, with minimal data acquisition, of filaments at many, local checkpoints in a device consisting of a potentially complex network of channels that guide filament motion. Here we develop such a detection system using actomyosin motility. Detection points consist of pairs of gold lines running perpendicular to nanochannels that guide motion of fluorescent actin filaments. Fluorescence interference contrast (FLIC) is used to locally enhance the signal at the gold lines. A cross-correlation method is used to suppress errors, allowing reliable detection of single or multiple filaments. Optimal device design parameters are discussed. The results open for automatic read-out of filament count and velocity in high-throughput motility assays, helping establish the viability of active, motor-driven on-chip applications. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3438381
- author
- Lard, Mercy
LU
; Siethoff, Lasse Ten
; Månsson, Alf
and Linke, Heiner
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 3
- article number
- 1092
- publisher
- Nature Publishing Group
- external identifiers
-
- wos:000313885400004
- pmid:23346350
- scopus:84873142047
- pmid:23346350
- ISSN
- 2045-2322
- DOI
- 10.1038/srep01092
- language
- English
- LU publication?
- yes
- id
- 0198aab0-9568-4a8a-ac5f-6f866b98c9ea (old id 3438381)
- date added to LUP
- 2016-04-01 13:41:16
- date last changed
- 2023-09-03 03:27:04
@article{0198aab0-9568-4a8a-ac5f-6f866b98c9ea,
abstract = {{Emerging concepts for on-chip biotechnologies aim to replace microfluidic flow by active, molecular-motor driven transport of cytoskeletal filaments, including applications in bio-simulation, biocomputation, diagnostics, and drug screening. Many of these applications require reliable detection, with minimal data acquisition, of filaments at many, local checkpoints in a device consisting of a potentially complex network of channels that guide filament motion. Here we develop such a detection system using actomyosin motility. Detection points consist of pairs of gold lines running perpendicular to nanochannels that guide motion of fluorescent actin filaments. Fluorescence interference contrast (FLIC) is used to locally enhance the signal at the gold lines. A cross-correlation method is used to suppress errors, allowing reliable detection of single or multiple filaments. Optimal device design parameters are discussed. The results open for automatic read-out of filament count and velocity in high-throughput motility assays, helping establish the viability of active, motor-driven on-chip applications.}},
author = {{Lard, Mercy and Siethoff, Lasse Ten and Månsson, Alf and Linke, Heiner}},
issn = {{2045-2322}},
language = {{eng}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Tracking actomyosin at fluorescence check points.}},
url = {{https://lup.lub.lu.se/search/files/3532583/3457790.pdf}},
doi = {{10.1038/srep01092}},
volume = {{3}},
year = {{2013}},
}