Expression of bovine calmodulin in tobacco plants confers faster germination on saline media
Olsson, Peter LU
; Lindberg, Jenny
LU
; Sommarin, Marianne
LU
; Persson, Staffan
LU
and Bülow, Leif
LU
(2004)
In Plant Science
166(6).
p.1595-1604
- Abstract
- Calmodulin is a Ca2+-dependent regulatory protein in eukaryotic cells involved in a variety of intracellular activities. Many responses to abiotic stresses involve signaling by Ca2+ and Ca2+/calmodulin regulation, including water and salinity stress. To investigate how calmodulin affects germination on media with high concentrations of NaCl, transgenic tobacco plants expressing heterologous calmodulin were generated and characterized. Transgenic tobacco seeds showed significantly shortened germination times on media containing varying salt (120-160 mM) and calcium (3 and 13 mM) concentrations, compared to control seeds. Using media containing 140 mM NaCl and 13 mM CaCl2, the average germination time was shortened by 2-3 days compared to... (More)
- Calmodulin is a Ca2+-dependent regulatory protein in eukaryotic cells involved in a variety of intracellular activities. Many responses to abiotic stresses involve signaling by Ca2+ and Ca2+/calmodulin regulation, including water and salinity stress. To investigate how calmodulin affects germination on media with high concentrations of NaCl, transgenic tobacco plants expressing heterologous calmodulin were generated and characterized. Transgenic tobacco seeds showed significantly shortened germination times on media containing varying salt (120-160 mM) and calcium (3 and 13 mM) concentrations, compared to control seeds. Using media containing 140 mM NaCl and 13 mM CaCl2, the average germination time was shortened by 2-3 days compared to control (P < 0.05). In addition, the germinating transgenic seeds contained higher transient levels of gamma-aminobutyric acid compared to control seeds (P < 0.05). The tobacco Ca2+/calmodulin-regulated glutamate decarboxylase, synthesizing gamma-aminobutyric acid may therefore be stimulated by the heterologous calmodulin. (C) 2004 Elsevier Ireland Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/138593
- author
- Olsson, Peter
LU
; Lindberg, Jenny
LU
; Sommarin, Marianne
LU
; Persson, Staffan
LU
and Bülow, Leif
LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- NaCl, Calcium, γ-Amino butyric acid, Glutamate decarboxylase, Nicotiana tabacum
- in
- Plant Science
- volume
- 166
- issue
- 6
- pages
- 1595 - 1604
- publisher
- Elsevier
- external identifiers
-
- wos:000221136600023
- scopus:1942477681
- ISSN
- 0168-9452
- DOI
- 10.1016/j.plantsci.2004.02.013
- language
- English
- LU publication?
- yes
- id
- 019e10f6-abc7-458a-bec3-44d261c3fa9c (old id 138593)
- date added to LUP
- 2016-04-01 15:19:06
- date last changed
- 2022-01-28 04:43:16
@article{019e10f6-abc7-458a-bec3-44d261c3fa9c,
abstract = {{Calmodulin is a Ca2+-dependent regulatory protein in eukaryotic cells involved in a variety of intracellular activities. Many responses to abiotic stresses involve signaling by Ca2+ and Ca2+/calmodulin regulation, including water and salinity stress. To investigate how calmodulin affects germination on media with high concentrations of NaCl, transgenic tobacco plants expressing heterologous calmodulin were generated and characterized. Transgenic tobacco seeds showed significantly shortened germination times on media containing varying salt (120-160 mM) and calcium (3 and 13 mM) concentrations, compared to control seeds. Using media containing 140 mM NaCl and 13 mM CaCl2, the average germination time was shortened by 2-3 days compared to control (P < 0.05). In addition, the germinating transgenic seeds contained higher transient levels of gamma-aminobutyric acid compared to control seeds (P < 0.05). The tobacco Ca2+/calmodulin-regulated glutamate decarboxylase, synthesizing gamma-aminobutyric acid may therefore be stimulated by the heterologous calmodulin. (C) 2004 Elsevier Ireland Ltd. All rights reserved.}},
author = {{Olsson, Peter and Lindberg, Jenny and Sommarin, Marianne and Persson, Staffan and Bülow, Leif}},
issn = {{0168-9452}},
keywords = {{NaCl; Calcium; γ-Amino butyric acid; Glutamate decarboxylase; Nicotiana tabacum}},
language = {{eng}},
number = {{6}},
pages = {{1595--1604}},
publisher = {{Elsevier}},
series = {{Plant Science}},
title = {{Expression of bovine calmodulin in tobacco plants confers faster germination on saline media}},
url = {{http://dx.doi.org/10.1016/j.plantsci.2004.02.013}},
doi = {{10.1016/j.plantsci.2004.02.013}},
volume = {{166}},
year = {{2004}},
}