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DBS-LC-MS/MS assay for caffeine : Validation and neonatal application

Bruschettini, Matteo LU orcid ; Barco, Sebastiano ; Romantsik, Olga LU ; Risso, Francesco ; Gennai, Iulian ; Chinea, Benito ; Ramenghi, Luca A. ; Tripodi, Gino and Cangemi, Giuliana (2016) In Bioanalysis 8(18). p.1893-1902
Abstract

Aim: DBS might be an appropriate microsampling technique for therapeutic drug monitoring of caffeine in infants. Nevertheless, its application presents several issues that still limit its use. This paper describes a validated DBS-LC-MS/MS method for caffeine. Results: The results of the method validation showed an hematocrit dependence. In the analysis of 96 paired plasma and DBS clinical samples, caffeine levels measured in DBS were statistically significantly lower than in plasma but the observed differences were independent from hematocrit. Conclusion: These results clearly showed the need for extensive validation with real-life samples for DBS-based methods. DBS-LC-MS/MS can be considered to be a good alternative to traditional... (More)

Aim: DBS might be an appropriate microsampling technique for therapeutic drug monitoring of caffeine in infants. Nevertheless, its application presents several issues that still limit its use. This paper describes a validated DBS-LC-MS/MS method for caffeine. Results: The results of the method validation showed an hematocrit dependence. In the analysis of 96 paired plasma and DBS clinical samples, caffeine levels measured in DBS were statistically significantly lower than in plasma but the observed differences were independent from hematocrit. Conclusion: These results clearly showed the need for extensive validation with real-life samples for DBS-based methods. DBS-LC-MS/MS can be considered to be a good alternative to traditional methods for therapeutic drug monitoring or PK studies in preterm infants.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
caffeine, DBS, LC-MS/MS, newborn, PK, preterm, therapeutic drug monitoring
in
Bioanalysis
volume
8
issue
18
pages
10 pages
publisher
Taylor & Francis
external identifiers
  • scopus:84984710567
  • pmid:27532249
  • wos:000382469400002
ISSN
1757-6180
DOI
10.4155/bio-2016-0127
language
English
LU publication?
yes
id
01bede13-133d-4041-a107-e17effa2d6d2
date added to LUP
2016-11-22 08:30:50
date last changed
2025-03-24 01:59:28
@article{01bede13-133d-4041-a107-e17effa2d6d2,
  abstract     = {{<p>Aim: DBS might be an appropriate microsampling technique for therapeutic drug monitoring of caffeine in infants. Nevertheless, its application presents several issues that still limit its use. This paper describes a validated DBS-LC-MS/MS method for caffeine. Results: The results of the method validation showed an hematocrit dependence. In the analysis of 96 paired plasma and DBS clinical samples, caffeine levels measured in DBS were statistically significantly lower than in plasma but the observed differences were independent from hematocrit. Conclusion: These results clearly showed the need for extensive validation with real-life samples for DBS-based methods. DBS-LC-MS/MS can be considered to be a good alternative to traditional methods for therapeutic drug monitoring or PK studies in preterm infants.</p>}},
  author       = {{Bruschettini, Matteo and Barco, Sebastiano and Romantsik, Olga and Risso, Francesco and Gennai, Iulian and Chinea, Benito and Ramenghi, Luca A. and Tripodi, Gino and Cangemi, Giuliana}},
  issn         = {{1757-6180}},
  keywords     = {{caffeine; DBS; LC-MS/MS; newborn; PK; preterm; therapeutic drug monitoring}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{18}},
  pages        = {{1893--1902}},
  publisher    = {{Taylor & Francis}},
  series       = {{Bioanalysis}},
  title        = {{DBS-LC-MS/MS assay for caffeine : Validation and neonatal application}},
  url          = {{http://dx.doi.org/10.4155/bio-2016-0127}},
  doi          = {{10.4155/bio-2016-0127}},
  volume       = {{8}},
  year         = {{2016}},
}