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Remote ischemic preconditioning protects human neural stem cells from oxidative stress

Motomura, Ayako; Shimizu, Mikiko; Kato, Akira; Motomura, Kazuya; Yamamichi, Akane; Koyama, Hiroko; Ohka, Fumiharu; Nishikawa, Tomohide; Nishimura, Yusuke and Hara, Masahito, et al. (2017) In Apoptosis p.1-9
Abstract

In previous clinical trials, we showed that remote ischemic preconditioning (rIPC) reduced myocardial damage in children undergoing treatment for congenital heart defects and postoperative renal failure in patients undergoing abdominal aortic aneurysm surgery. In rabbit experiments, pre-treatment with plasma and plasma dialysate (obtained using 15-kDa cut-off dialysis membrane) from donor rabbits subjected to rIPC similarly protected against cardiac infarction. However, the protective substances containing in rIPC plasma have been unknown. In the present study, we showed that rIPC plasma exerted anti-apoptotic and anti-oxidative effects on human neural stem cells under oxygen glucose deprivation (OGD) that mimics brain ischemia.... (More)

In previous clinical trials, we showed that remote ischemic preconditioning (rIPC) reduced myocardial damage in children undergoing treatment for congenital heart defects and postoperative renal failure in patients undergoing abdominal aortic aneurysm surgery. In rabbit experiments, pre-treatment with plasma and plasma dialysate (obtained using 15-kDa cut-off dialysis membrane) from donor rabbits subjected to rIPC similarly protected against cardiac infarction. However, the protective substances containing in rIPC plasma have been unknown. In the present study, we showed that rIPC plasma exerted anti-apoptotic and anti-oxidative effects on human neural stem cells under oxygen glucose deprivation (OGD) that mimics brain ischemia. Additionally, we applied the sample to the liquid chromatography integrated with mass spectrometry to identify candidate key molecules in the rIPC plasma and determine its role in protecting neural stem cells from OGD-induced cell death. Thioredoxin increased significantly after rIPC compared to pre-IPC. Pretreatment with thioredoxin, the antioxidant protein, markedly protected human neural stem cells from OGD-induced cell death. The effect of thioredoxin on brain ischemia in animals should be further evaluated. However, the present study first evaluated the effect of rIPC in the ischemic cellular model.

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publication status
in press
subject
keywords
Neural stem cells, Oxidative stress, Oxygen glucose deprivation (OGD), Remote ischemic preconditioning
in
Apoptosis
pages
9 pages
publisher
Springer
external identifiers
  • scopus:85029896241
  • wos:000413013100004
ISSN
1360-8185
DOI
10.1007/s10495-017-1425-8
language
English
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no
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01e156b3-4ae4-4746-9876-70f6c0fb2071
date added to LUP
2017-10-06 11:42:35
date last changed
2018-01-16 13:21:31
@article{01e156b3-4ae4-4746-9876-70f6c0fb2071,
  abstract     = {<p>In previous clinical trials, we showed that remote ischemic preconditioning (rIPC) reduced myocardial damage in children undergoing treatment for congenital heart defects and postoperative renal failure in patients undergoing abdominal aortic aneurysm surgery. In rabbit experiments, pre-treatment with plasma and plasma dialysate (obtained using 15-kDa cut-off dialysis membrane) from donor rabbits subjected to rIPC similarly protected against cardiac infarction. However, the protective substances containing in rIPC plasma have been unknown. In the present study, we showed that rIPC plasma exerted anti-apoptotic and anti-oxidative effects on human neural stem cells under oxygen glucose deprivation (OGD) that mimics brain ischemia. Additionally, we applied the sample to the liquid chromatography integrated with mass spectrometry to identify candidate key molecules in the rIPC plasma and determine its role in protecting neural stem cells from OGD-induced cell death. Thioredoxin increased significantly after rIPC compared to pre-IPC. Pretreatment with thioredoxin, the antioxidant protein, markedly protected human neural stem cells from OGD-induced cell death. The effect of thioredoxin on brain ischemia in animals should be further evaluated. However, the present study first evaluated the effect of rIPC in the ischemic cellular model.</p>},
  author       = {Motomura, Ayako and Shimizu, Mikiko and Kato, Akira and Motomura, Kazuya and Yamamichi, Akane and Koyama, Hiroko and Ohka, Fumiharu and Nishikawa, Tomohide and Nishimura, Yusuke and Hara, Masahito and Fukuda, Tetsuya and Bando, Yasuhiko and Nishimura, Toshihide and Wakabayashi, Toshihiko and Natsume, Atsushi},
  issn         = {1360-8185},
  keyword      = {Neural stem cells,Oxidative stress,Oxygen glucose deprivation (OGD),Remote ischemic preconditioning},
  language     = {eng},
  month        = {09},
  pages        = {1--9},
  publisher    = {Springer},
  series       = {Apoptosis},
  title        = {Remote ischemic preconditioning protects human neural stem cells from oxidative stress},
  url          = {http://dx.doi.org/10.1007/s10495-017-1425-8},
  year         = {2017},
}