Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Continuous selection pressure to improve temperature acclimation of Tisochrysis lutea

Bonnefond, Hubert ; Grimaud, Ghjuvan LU orcid ; Rumin, Judith ; Bougaran, Gaël ; Talec, Amélie ; Gachelin, Manon ; Boutoute, Marc ; Pruvost, Eric ; Bernard, Olivier and Sciandra, Antoine (2017) In PLoS ONE 12(9).
Abstract

Temperature plays a key role in outdoor industrial cultivation of microalgae. Improving the thermal tolerance of microalgae to both daily and seasonal temperature fluctuations can thus contribute to increase their annual productivity. A long term selection experiment was carried out to increase the thermal niche (temperature range for which the growth is possible) of a neutral lipid overproducing strain of Tisochrysis lutea. The experimental protocol consisted to submit cells to daily variations of temperature for 7 months. The stress intensity, defined as the amplitude of daily temperature variations, was progressively increased along successive selection cycles. Only the amplitude of the temperature variations were increased, the... (More)

Temperature plays a key role in outdoor industrial cultivation of microalgae. Improving the thermal tolerance of microalgae to both daily and seasonal temperature fluctuations can thus contribute to increase their annual productivity. A long term selection experiment was carried out to increase the thermal niche (temperature range for which the growth is possible) of a neutral lipid overproducing strain of Tisochrysis lutea. The experimental protocol consisted to submit cells to daily variations of temperature for 7 months. The stress intensity, defined as the amplitude of daily temperature variations, was progressively increased along successive selection cycles. Only the amplitude of the temperature variations were increased, the daily average temperature was kept constant along the experiment. This protocol resulted in a thermal niche increase by 3C (+16.5%), with an enhancement by 9% of the maximal growth rate. The selection process also affected T. lutea physiology, with a feature generally observed for ‘cold-temperature’ type of adaptation. The amount of total and neutral lipids was significantly increased, and eventually productivity was increased by 34%. This seven month selection experiment, carried out in a highly dynamic environment, challenges some of the hypotheses classically advanced to explain the temperature response of microalgae.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
PLoS ONE
volume
12
issue
9
article number
e0183547
publisher
Public Library of Science (PLoS)
external identifiers
  • pmid:28902878
  • scopus:85029420332
ISSN
1932-6203
DOI
10.1371/journal.pone.0183547
language
English
LU publication?
no
additional info
Publisher Copyright: Copyright: © 2017 Bonnefond et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
id
01feba6c-1069-492b-b749-010dbc7012c4
date added to LUP
2024-11-25 16:12:10
date last changed
2025-01-06 19:21:31
@article{01feba6c-1069-492b-b749-010dbc7012c4,
  abstract     = {{<p>Temperature plays a key role in outdoor industrial cultivation of microalgae. Improving the thermal tolerance of microalgae to both daily and seasonal temperature fluctuations can thus contribute to increase their annual productivity. A long term selection experiment was carried out to increase the thermal niche (temperature range for which the growth is possible) of a neutral lipid overproducing strain of Tisochrysis lutea. The experimental protocol consisted to submit cells to daily variations of temperature for 7 months. The stress intensity, defined as the amplitude of daily temperature variations, was progressively increased along successive selection cycles. Only the amplitude of the temperature variations were increased, the daily average temperature was kept constant along the experiment. This protocol resulted in a thermal niche increase by 3C (+16.5%), with an enhancement by 9% of the maximal growth rate. The selection process also affected T. lutea physiology, with a feature generally observed for ‘cold-temperature’ type of adaptation. The amount of total and neutral lipids was significantly increased, and eventually productivity was increased by 34%. This seven month selection experiment, carried out in a highly dynamic environment, challenges some of the hypotheses classically advanced to explain the temperature response of microalgae.</p>}},
  author       = {{Bonnefond, Hubert and Grimaud, Ghjuvan and Rumin, Judith and Bougaran, Gaël and Talec, Amélie and Gachelin, Manon and Boutoute, Marc and Pruvost, Eric and Bernard, Olivier and Sciandra, Antoine}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  number       = {{9}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{Continuous selection pressure to improve temperature acclimation of <i>Tisochrysis lutea</i>}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0183547}},
  doi          = {{10.1371/journal.pone.0183547}},
  volume       = {{12}},
  year         = {{2017}},
}