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Microelectrode array analysis of hippocampal network dynamics following theta-burst stimulation via current source density reconstruction by Gaussian interpolation

Kim, Hyun-Bum ; Oh, Tong-In ; Swanberg, Kelley M LU orcid ; Lee, Mun-Bae ; Kim, Tae-Woo ; Woo, Eung-Je ; Park, Ji-Ho and Kwon, Oh-In (2016) In Journal of Neuroscience Methods 264. p.1-10
Abstract

BACKGROUND: Multielectrode arrays (MEAs) have been used to understand electrophysiological network dynamics by recording real-time activity in groups of cells. The extent to which the collection of such data enables hypothesis testing on the level of circuits and networks depends largely on the sophistication of the analyses applied.

NEW METHOD: We studied the systemic temporal variations of endogenous signaling within an organotypic hippocampal network following theta-burst stimulation (TBS) to the Schaffer collateral-commissural pathways. The recovered current source density (CSD) information from the raw grid of extracellular potentials by using a Gaussian interpolation method increases spatial resolution and avoids border... (More)

BACKGROUND: Multielectrode arrays (MEAs) have been used to understand electrophysiological network dynamics by recording real-time activity in groups of cells. The extent to which the collection of such data enables hypothesis testing on the level of circuits and networks depends largely on the sophistication of the analyses applied.

NEW METHOD: We studied the systemic temporal variations of endogenous signaling within an organotypic hippocampal network following theta-burst stimulation (TBS) to the Schaffer collateral-commissural pathways. The recovered current source density (CSD) information from the raw grid of extracellular potentials by using a Gaussian interpolation method increases spatial resolution and avoids border artifacts by numerical differentials.

RESULTS: We compared total sink and source currents in DG, CA3, and CA1; calculated accumulated correlation coefficients to compare pre- with post-stimulation CSD dynamics in each region; and reconstructed functional connectivity maps for regional cross-correlations with respect to temporal CSD variations. The functional connectivity maps for potential correlations pre- and post-TBS were compared to investigate the neural network as a whole, revealing differences post-TBS.

COMPARISON WITH EXISTING METHOD(S): Previous MEA work on plasticity in hippocampal evoked potentials has focused on synchronicity across the hippocampus within isolated subregions. Such analyses ignore the complex relationships among diverse components of the hippocampal circuitry, thus failing to capture network-level behaviors integral to understanding hippocampal function.

CONCLUSIONS: The proposed method of recovering current source density to examine whole-hippocampal function is sensitive to experimental manipulation and is worth further examination in the context of network-level analyses of neural signaling.

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author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Data Interpretation, Statistical, Electrophysiological Phenomena, Evoked Potentials/physiology, Hippocampus/physiology, Microelectrodes, Nerve Net/physiology, Rats, Rats, Sprague-Dawley, Transcranial Magnetic Stimulation/methods
in
Journal of Neuroscience Methods
volume
264
pages
1 - 10
publisher
Elsevier
external identifiers
  • pmid:26880160
  • scopus:84959322134
ISSN
1872-678X
DOI
10.1016/j.jneumeth.2016.02.011
language
English
LU publication?
no
additional info
Copyright © 2016 Elsevier B.V. All rights reserved.
id
021d4c6d-0d91-48d8-a315-da7466d20428
date added to LUP
2023-09-18 15:03:40
date last changed
2023-09-20 07:38:30
@article{021d4c6d-0d91-48d8-a315-da7466d20428,
  abstract     = {{<p>BACKGROUND: Multielectrode arrays (MEAs) have been used to understand electrophysiological network dynamics by recording real-time activity in groups of cells. The extent to which the collection of such data enables hypothesis testing on the level of circuits and networks depends largely on the sophistication of the analyses applied.</p><p>NEW METHOD: We studied the systemic temporal variations of endogenous signaling within an organotypic hippocampal network following theta-burst stimulation (TBS) to the Schaffer collateral-commissural pathways. The recovered current source density (CSD) information from the raw grid of extracellular potentials by using a Gaussian interpolation method increases spatial resolution and avoids border artifacts by numerical differentials.</p><p>RESULTS: We compared total sink and source currents in DG, CA3, and CA1; calculated accumulated correlation coefficients to compare pre- with post-stimulation CSD dynamics in each region; and reconstructed functional connectivity maps for regional cross-correlations with respect to temporal CSD variations. The functional connectivity maps for potential correlations pre- and post-TBS were compared to investigate the neural network as a whole, revealing differences post-TBS.</p><p>COMPARISON WITH EXISTING METHOD(S): Previous MEA work on plasticity in hippocampal evoked potentials has focused on synchronicity across the hippocampus within isolated subregions. Such analyses ignore the complex relationships among diverse components of the hippocampal circuitry, thus failing to capture network-level behaviors integral to understanding hippocampal function.</p><p>CONCLUSIONS: The proposed method of recovering current source density to examine whole-hippocampal function is sensitive to experimental manipulation and is worth further examination in the context of network-level analyses of neural signaling.</p>}},
  author       = {{Kim, Hyun-Bum and Oh, Tong-In and Swanberg, Kelley M and Lee, Mun-Bae and Kim, Tae-Woo and Woo, Eung-Je and Park, Ji-Ho and Kwon, Oh-In}},
  issn         = {{1872-678X}},
  keywords     = {{Animals; Data Interpretation, Statistical; Electrophysiological Phenomena; Evoked Potentials/physiology; Hippocampus/physiology; Microelectrodes; Nerve Net/physiology; Rats; Rats, Sprague-Dawley; Transcranial Magnetic Stimulation/methods}},
  language     = {{eng}},
  month        = {{05}},
  pages        = {{1--10}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Neuroscience Methods}},
  title        = {{Microelectrode array analysis of hippocampal network dynamics following theta-burst stimulation via current source density reconstruction by Gaussian interpolation}},
  url          = {{http://dx.doi.org/10.1016/j.jneumeth.2016.02.011}},
  doi          = {{10.1016/j.jneumeth.2016.02.011}},
  volume       = {{264}},
  year         = {{2016}},
}