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3He MRI-based assessment of posture-dependent regional ventilation gradients in rats

Månsson, Sven LU ; Deninger, Anselm J; Magnusson, Peter LU ; Pettersson, Göran; Olsson, Lars E LU ; Hansson, Georg; Wollmer, Per LU and Golman, Klaes LU (2005) In Journal of Applied Physiology 98(6). p.67-2259
Abstract

A recently developed method for quantitative assessment of regional lung ventilation was employed for the study of posture-dependent ventilation differences in rats. The measurement employed hyperpolarized (3)He MRI to detect the build-up of the signal intensity after increasing numbers of (3)He breaths, which allowed for computation of a regional ventilation parameter. A group of six anesthetized rats was studied in both supine and prone postures. Three-dimensional maps of the ventilation parameter were obtained with high spatial resolution (voxel volume approximately 2 mm(3)). Vertical (dorsal-ventral) gradients of the ventilation index, defined as the regional ventilation normalized by the average ventilation within the whole lung,... (More)

A recently developed method for quantitative assessment of regional lung ventilation was employed for the study of posture-dependent ventilation differences in rats. The measurement employed hyperpolarized (3)He MRI to detect the build-up of the signal intensity after increasing numbers of (3)He breaths, which allowed for computation of a regional ventilation parameter. A group of six anesthetized rats was studied in both supine and prone postures. Three-dimensional maps of the ventilation parameter were obtained with high spatial resolution (voxel volume approximately 2 mm(3)). Vertical (dorsal-ventral) gradients of the ventilation index, defined as the regional ventilation normalized by the average ventilation within the whole lung, were investigated. Variations in the regional distribution of the ventilation parameter, as well as of the ventilation index, could be detected, depending on the posture of the rats. In supine posture, ventilation was elevated in the dependent parts of the lungs, with a linear gradient of the ventilation index of -0.11 +/- 0.03 cm(-1). In prone posture, the distribution of ventilation was more uniform, with a significantly (P < 0.001) smaller gradient of the ventilation index of -0.01 +/- 0.02 cm(-1). It is concluded that the (3)He MRI-based method can detect and quantify regional ventilation gradients in animals as small as the rat and that these gradients depend on prone or supine posture of the animal.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Administration, Inhalation, Algorithms, Animals, Contrast Media, Helium, Image Interpretation, Computer-Assisted, Imaging, Three-Dimensional, Isotopes, Lung, Magnetic Resonance Imaging, Men, Posture, Prone Position, Pulmonary Ventilation, Rats, Rats, Sprague-Dawley, Supine Position
in
Journal of Applied Physiology
volume
98
issue
6
pages
9 pages
publisher
American Physiological Society
external identifiers
  • scopus:19444367336
ISSN
8750-7587
DOI
10.1152/japplphysiol.00245.2004
language
English
LU publication?
yes
id
0232b463-f96d-473b-bc5c-ef65ff88b674
date added to LUP
2016-08-16 13:58:09
date last changed
2017-01-01 08:31:51
@article{0232b463-f96d-473b-bc5c-ef65ff88b674,
  abstract     = {<p>A recently developed method for quantitative assessment of regional lung ventilation was employed for the study of posture-dependent ventilation differences in rats. The measurement employed hyperpolarized (3)He MRI to detect the build-up of the signal intensity after increasing numbers of (3)He breaths, which allowed for computation of a regional ventilation parameter. A group of six anesthetized rats was studied in both supine and prone postures. Three-dimensional maps of the ventilation parameter were obtained with high spatial resolution (voxel volume approximately 2 mm(3)). Vertical (dorsal-ventral) gradients of the ventilation index, defined as the regional ventilation normalized by the average ventilation within the whole lung, were investigated. Variations in the regional distribution of the ventilation parameter, as well as of the ventilation index, could be detected, depending on the posture of the rats. In supine posture, ventilation was elevated in the dependent parts of the lungs, with a linear gradient of the ventilation index of -0.11 +/- 0.03 cm(-1). In prone posture, the distribution of ventilation was more uniform, with a significantly (P &lt; 0.001) smaller gradient of the ventilation index of -0.01 +/- 0.02 cm(-1). It is concluded that the (3)He MRI-based method can detect and quantify regional ventilation gradients in animals as small as the rat and that these gradients depend on prone or supine posture of the animal.</p>},
  author       = {Månsson, Sven and Deninger, Anselm J and Magnusson, Peter and Pettersson, Göran and Olsson, Lars E and Hansson, Georg and Wollmer, Per and Golman, Klaes},
  issn         = {8750-7587},
  keyword      = {Administration, Inhalation,Algorithms,Animals,Contrast Media,Helium,Image Interpretation, Computer-Assisted,Imaging, Three-Dimensional,Isotopes,Lung,Magnetic Resonance Imaging,Men,Posture,Prone Position,Pulmonary Ventilation,Rats,Rats, Sprague-Dawley,Supine Position},
  language     = {eng},
  number       = {6},
  pages        = {67--2259},
  publisher    = {American Physiological Society},
  series       = {Journal of Applied Physiology},
  title        = {3He MRI-based assessment of posture-dependent regional ventilation gradients in rats},
  url          = {http://dx.doi.org/10.1152/japplphysiol.00245.2004},
  volume       = {98},
  year         = {2005},
}