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Radioimmunoassay detects the frequent occurrence of autoantibodies to the mr 65,000 isoform of glutamic acid decarboxylase in japanese insulin-dependent diabetes

Falorni, A. ; Grubin, C. E. ; Takei, I. ; Shimada, A. ; Kasuga, A. ; Maruyama, T. ; Ozawa, Y. ; Kasatani, T. ; Saruta, T. and Li, L. , et al. (1994) In Autoimmunity 19(2). p.113-125
Abstract

Glutamic acid decarboxylase antibodies (GAD65Ab) are common in new onset Caucasian insulin-dependent diabetic (IDDM) patients but it is unclear if this marker is also prevalent in patients of other ethnic backgrounds. We determined antibodies against human recombinant GAD in Japanese diabetic patients using a radioimmunoassay with competition between in vitro translated 35S-GAD65 and non-labelled recombinant human GAD65 (rhGAD65). GAD67 antibodies (GAD67Ab) were similarly analyzed but without antigen competition. In 73 Japanese diabetic patients, GAD65Ab were found in 11/16 (69% of patients with short-duration (less than 5 yrs) IDDM, 6/23 (26% with long-duration (5 or more yrs) IDDM and 10/20 (50% with slowly progressive... (More)

Glutamic acid decarboxylase antibodies (GAD65Ab) are common in new onset Caucasian insulin-dependent diabetic (IDDM) patients but it is unclear if this marker is also prevalent in patients of other ethnic backgrounds. We determined antibodies against human recombinant GAD in Japanese diabetic patients using a radioimmunoassay with competition between in vitro translated 35S-GAD65 and non-labelled recombinant human GAD65 (rhGAD65). GAD67 antibodies (GAD67Ab) were similarly analyzed but without antigen competition. In 73 Japanese diabetic patients, GAD65Ab were found in 11/16 (69% of patients with short-duration (less than 5 yrs) IDDM, 6/23 (26% with long-duration (5 or more yrs) IDDM and 10/20 (50% with slowly progressive diabetes. High GAD65Ab levels were associated with concomitant autoimmune diseases (p = 0.021). GAD67Ab were found in 4/16 (25% of patients with short-duration IDDM, 3/23 (13% with long-duration IDDM and 2/20 (10% with slowly progressive diabetes. In 14 non-insulin dependent diabetic (NIDDM) patients, GAD65Ab and GAD67Ab were not found (0/14) and 1/50 (2% healthy controls were positive in either assay. Among the GAD67Ab-positive samples, 8/9 (88% were also high level GAD65Ab positive, 7/9 (77% were displaced by an excess of rhGAD65 and the antibody levels correlated (r2 = 0.573; p = 0.003). Our data are consistent with a strong association of GAD65Ab also in Japanese IDDM, and suggest that, when present, GAD67Ab are frequently directed to epitope(s) common to GAD65 and GAD67.

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publishing date
type
Contribution to journal
publication status
published
keywords
Autoantigen, Autoimmunity, Glutamic acid decarboxylase, Insulin-dependent diabetes, Radioimmunoassay
in
Autoimmunity
volume
19
issue
2
pages
113 - 125
publisher
Taylor & Francis
external identifiers
  • pmid:7772701
  • scopus:0028658449
ISSN
0891-6934
DOI
10.3109/08916939409009539
language
English
LU publication?
no
id
023a8a7f-4b92-4295-a7b3-305ae36ab23c
date added to LUP
2019-09-11 09:03:29
date last changed
2021-01-03 07:37:14
@article{023a8a7f-4b92-4295-a7b3-305ae36ab23c,
  abstract     = {<p>Glutamic acid decarboxylase antibodies (GAD65Ab) are common in new onset Caucasian insulin-dependent diabetic (IDDM) patients but it is unclear if this marker is also prevalent in patients of other ethnic backgrounds. We determined antibodies against human recombinant GAD in Japanese diabetic patients using a radioimmunoassay with competition between in vitro translated <sup>35</sup>S-GAD65 and non-labelled recombinant human GAD65 (rhGAD65). GAD67 antibodies (GAD67Ab) were similarly analyzed but without antigen competition. In 73 Japanese diabetic patients, GAD65Ab were found in 11/16 (69% of patients with short-duration (less than 5 yrs) IDDM, 6/23 (26% with long-duration (5 or more yrs) IDDM and 10/20 (50% with slowly progressive diabetes. High GAD65Ab levels were associated with concomitant autoimmune diseases (p = 0.021). GAD67Ab were found in 4/16 (25% of patients with short-duration IDDM, 3/23 (13% with long-duration IDDM and 2/20 (10% with slowly progressive diabetes. In 14 non-insulin dependent diabetic (NIDDM) patients, GAD65Ab and GAD67Ab were not found (0/14) and 1/50 (2% healthy controls were positive in either assay. Among the GAD67Ab-positive samples, 8/9 (88% were also high level GAD65Ab positive, 7/9 (77% were displaced by an excess of rhGAD65 and the antibody levels correlated (r<sup>2</sup> = 0.573; p = 0.003). Our data are consistent with a strong association of GAD65Ab also in Japanese IDDM, and suggest that, when present, GAD67Ab are frequently directed to epitope(s) common to GAD65 and GAD67.</p>},
  author       = {Falorni, A. and Grubin, C. E. and Takei, I. and Shimada, A. and Kasuga, A. and Maruyama, T. and Ozawa, Y. and Kasatani, T. and Saruta, T. and Li, L. and Lernmark, A.},
  issn         = {0891-6934},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {113--125},
  publisher    = {Taylor & Francis},
  series       = {Autoimmunity},
  title        = {Radioimmunoassay detects the frequent occurrence of autoantibodies to the mr 65,000 isoform of glutamic acid decarboxylase in japanese insulin-dependent diabetes},
  url          = {http://dx.doi.org/10.3109/08916939409009539},
  doi          = {10.3109/08916939409009539},
  volume       = {19},
  year         = {1994},
}