Effect of periodate oxidation and borohydride reduction on the chemical structure and chain conformation of arabinoxylan
(2026) In Carbohydrate Polymers 376.- Abstract
The effect of periodate oxidation and borohydride reduction on arabinoxylan (AX) in aqueous solution was investigated using 13C NMR spectroscopy and small-angle X-ray scattering (SAXS). AX consists of a xylose backbone, which is mono- or di-substituted with arabinose. We show that at a ring-opening modification degree of 21%, periodate oxidation occurs predominantly on arabinose, and shows a preference for arabinose linked to di-substituted xylose units over the mono-substituted. With a higher degree of modification of 33%, arabinose at the mono-substituted position and unsubstituted xylose units are also modified. At 33% a large portion of oxidizable AX residues have been ring-opened, yet SAXS reveals no significant changes... (More)
The effect of periodate oxidation and borohydride reduction on arabinoxylan (AX) in aqueous solution was investigated using 13C NMR spectroscopy and small-angle X-ray scattering (SAXS). AX consists of a xylose backbone, which is mono- or di-substituted with arabinose. We show that at a ring-opening modification degree of 21%, periodate oxidation occurs predominantly on arabinose, and shows a preference for arabinose linked to di-substituted xylose units over the mono-substituted. With a higher degree of modification of 33%, arabinose at the mono-substituted position and unsubstituted xylose units are also modified. At 33% a large portion of oxidizable AX residues have been ring-opened, yet SAXS reveals no significant changes in chain conformation and only a minor reduction (10%) in persistence length for the oxidized-reduced dialcohol AX. With increasing degree of oxidation, dialdehyde AX instead show an increasing tendency for aggregation, attributed to chain cross-linking. Upon reduction of the dialdehyde and cross-linked groups to dialcohol AX, the chains revert back to having repulsive interactions, acting as chains in a good solvent environment.
(Less)
- author
- Janewithayapun, Ratchawit ; Karlsson, Hampus ; Herranz-Trillo, Fátima LU ; Terry, Ann E. LU ; Evenäs, Lars ; Cousin, Fabrice and Ström, Anna
- organization
- publishing date
- 2026-03-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Linkage analysis, NMR, Polysaccharide conformation, Small-angle X-ray scattering, Wheat bran
- in
- Carbohydrate Polymers
- volume
- 376
- article number
- 124811
- publisher
- Elsevier
- external identifiers
-
- pmid:41611428
- scopus:105025455292
- ISSN
- 0144-8617
- DOI
- 10.1016/j.carbpol.2025.124811
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2025 The Authors.
- id
- 02433c03-a9ab-48bf-abfb-3ef8ec6318a2
- date added to LUP
- 2026-03-23 13:39:55
- date last changed
- 2026-06-01 20:30:42
@article{02433c03-a9ab-48bf-abfb-3ef8ec6318a2,
abstract = {{<p>The effect of periodate oxidation and borohydride reduction on arabinoxylan (AX) in aqueous solution was investigated using <sup>13</sup>C NMR spectroscopy and small-angle X-ray scattering (SAXS). AX consists of a xylose backbone, which is mono- or di-substituted with arabinose. We show that at a ring-opening modification degree of 21%, periodate oxidation occurs predominantly on arabinose, and shows a preference for arabinose linked to di-substituted xylose units over the mono-substituted. With a higher degree of modification of 33%, arabinose at the mono-substituted position and unsubstituted xylose units are also modified. At 33% a large portion of oxidizable AX residues have been ring-opened, yet SAXS reveals no significant changes in chain conformation and only a minor reduction (10%) in persistence length for the oxidized-reduced dialcohol AX. With increasing degree of oxidation, dialdehyde AX instead show an increasing tendency for aggregation, attributed to chain cross-linking. Upon reduction of the dialdehyde and cross-linked groups to dialcohol AX, the chains revert back to having repulsive interactions, acting as chains in a good solvent environment.</p>}},
author = {{Janewithayapun, Ratchawit and Karlsson, Hampus and Herranz-Trillo, Fátima and Terry, Ann E. and Evenäs, Lars and Cousin, Fabrice and Ström, Anna}},
issn = {{0144-8617}},
keywords = {{Linkage analysis; NMR; Polysaccharide conformation; Small-angle X-ray scattering; Wheat bran}},
language = {{eng}},
month = {{03}},
publisher = {{Elsevier}},
series = {{Carbohydrate Polymers}},
title = {{Effect of periodate oxidation and borohydride reduction on the chemical structure and chain conformation of arabinoxylan}},
url = {{http://dx.doi.org/10.1016/j.carbpol.2025.124811}},
doi = {{10.1016/j.carbpol.2025.124811}},
volume = {{376}},
year = {{2026}},
}