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Electrochemically Assisted Biosensors

Xie, Bin LU and Danielsson, Bengt LU (1994) p.246-246
Abstract
This chapter describes a study focusing on electrochemically assisted biosensors. Electrochemical regeneration of electron-transfer mediators or cofactors, such as NAD+ has been extensively applied in redox reactions catalyzed by oxidoreductases. This electrochemical regeneration shows many advantages in use of amperometric biosensors, such as extended linear range. To demonstrate the feasibility of constructing such a biosensor, a ferrocene-mediated thermal glucose sensor was fabricated. In this study, the heat production (Δq) accompanying the glucose oxidation catalyzed by glucose oxidase was detected, as opposed to the electrical signal (current or potential change) that is normally measured in electrochemical analysis. Ferrocene was... (More)
This chapter describes a study focusing on electrochemically assisted biosensors. Electrochemical regeneration of electron-transfer mediators or cofactors, such as NAD+ has been extensively applied in redox reactions catalyzed by oxidoreductases. This electrochemical regeneration shows many advantages in use of amperometric biosensors, such as extended linear range. To demonstrate the feasibility of constructing such a biosensor, a ferrocene-mediated thermal glucose sensor was fabricated. In this study, the heat production (Δq) accompanying the glucose oxidation catalyzed by glucose oxidase was detected, as opposed to the electrical signal (current or potential change) that is normally measured in electrochemical analysis. Ferrocene was used as electron-transfer mediator to extend the linear range up to 20 mM glucose. Fiber optic biosensors for fluorescence measurement, such as NADH-based biosensors, could be modified in a similar way, if the electrochemical regeneration of NAD+ in the dehydrogenase-catalyzed reaction can be accomplished in the fiberoptic measurement system. Therefore, the sensitivity and the linear range of the sensors could be improved because recycling of NAD+/NADH can be electrochemically sustained. (Less)
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author
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type
Contribution to conference
publication status
published
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pages
246 - 246
DOI
10.1016/B978-1-85617-242-4.50203-2
language
English
LU publication?
yes
id
0256cc31-5fac-46fa-aa0f-94c783c10270
date added to LUP
2021-10-27 19:17:06
date last changed
2021-11-01 13:41:49
@misc{0256cc31-5fac-46fa-aa0f-94c783c10270,
  abstract     = {{This chapter describes a study focusing on electrochemically assisted biosensors. Electrochemical regeneration of electron-transfer mediators or cofactors, such as NAD+ has been extensively applied in redox reactions catalyzed by oxidoreductases. This electrochemical regeneration shows many advantages in use of amperometric biosensors, such as extended linear range. To demonstrate the feasibility of constructing such a biosensor, a ferrocene-mediated thermal glucose sensor was fabricated. In this study, the heat production (Δq) accompanying the glucose oxidation catalyzed by glucose oxidase was detected, as opposed to the electrical signal (current or potential change) that is normally measured in electrochemical analysis. Ferrocene was used as electron-transfer mediator to extend the linear range up to 20 mM glucose. Fiber optic biosensors for fluorescence measurement, such as NADH-based biosensors, could be modified in a similar way, if the electrochemical regeneration of NAD+ in the dehydrogenase-catalyzed reaction can be accomplished in the fiberoptic measurement system. Therefore, the sensitivity and the linear range of the sensors could be improved because recycling of NAD+/NADH can be electrochemically sustained.}},
  author       = {{Xie, Bin and Danielsson, Bengt}},
  language     = {{eng}},
  pages        = {{246--246}},
  title        = {{Electrochemically Assisted Biosensors}},
  url          = {{http://dx.doi.org/10.1016/B978-1-85617-242-4.50203-2}},
  doi          = {{10.1016/B978-1-85617-242-4.50203-2}},
  year         = {{1994}},
}