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Structural stability of SoPIP2;1 aquaporin under reconstitution in polymersomes

González-Pérez, Alfredo LU ; Persson, Kenneth M. LU and Taboada, Pablo (2018) In Journal of Molecular Liquids 257. p.26-31
Abstract

In the present paper we show the preparation of polymersomes using ABA triblock copolymers with poly(dimethylsiloxane) and poly(2-methyloxazoline) blocks with the incorporation of SoPIP2;1 aquaporin in their structure. The presence of the protein in the polymersome membrane was demonstrated using imaging techniques, and the conformation of the protein into the membranes was assessed using Raman spectroscopy and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The results suggest the presence of SoPIP2;1 aquaporin in a functional form which could be used for the preparation of Aquaporin-based membranes for water purification via reverse osmosis without the needed of liposome or lipidic-based reconstitution... (More)

In the present paper we show the preparation of polymersomes using ABA triblock copolymers with poly(dimethylsiloxane) and poly(2-methyloxazoline) blocks with the incorporation of SoPIP2;1 aquaporin in their structure. The presence of the protein in the polymersome membrane was demonstrated using imaging techniques, and the conformation of the protein into the membranes was assessed using Raman spectroscopy and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The results suggest the presence of SoPIP2;1 aquaporin in a functional form which could be used for the preparation of Aquaporin-based membranes for water purification via reverse osmosis without the needed of liposome or lipidic-based reconstitution media.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aquaporin SoPIP2;1, Block copolymers, Membranes, Nanofiltration, Polymersomes
in
Journal of Molecular Liquids
volume
257
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85042492269
ISSN
0167-7322
DOI
10.1016/j.molliq.2018.02.092
language
English
LU publication?
yes
id
02819d29-00dd-4a47-bb6d-dd37bbd34def
date added to LUP
2018-03-08 10:08:09
date last changed
2018-05-29 10:22:53
@article{02819d29-00dd-4a47-bb6d-dd37bbd34def,
  abstract     = {<p>In the present paper we show the preparation of polymersomes using ABA triblock copolymers with poly(dimethylsiloxane) and poly(2-methyloxazoline) blocks with the incorporation of SoPIP2;1 aquaporin in their structure. The presence of the protein in the polymersome membrane was demonstrated using imaging techniques, and the conformation of the protein into the membranes was assessed using Raman spectroscopy and attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The results suggest the presence of SoPIP2;1 aquaporin in a functional form which could be used for the preparation of Aquaporin-based membranes for water purification via reverse osmosis without the needed of liposome or lipidic-based reconstitution media.</p>},
  author       = {González-Pérez, Alfredo and Persson, Kenneth M. and Taboada, Pablo},
  issn         = {0167-7322},
  keyword      = {Aquaporin SoPIP2;1,Block copolymers,Membranes,Nanofiltration,Polymersomes},
  language     = {eng},
  month        = {05},
  pages        = {26--31},
  publisher    = {Elsevier},
  series       = {Journal of Molecular Liquids},
  title        = {Structural stability of SoPIP2;1 aquaporin under reconstitution in polymersomes},
  url          = {http://dx.doi.org/10.1016/j.molliq.2018.02.092},
  volume       = {257},
  year         = {2018},
}