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Feasibility of in vivo measurement of glucose metabolism in the mouse hypothalamus by 1 H-[13 C] MRS at 14.1T

Lizarbe, Blanca ; Lei, Hongxia ; Duarte, Joao M N LU ; Lanz, Bernard ; Cherix, Antoine and Gruetter, Rolf (2018) In Magnetic Resonance in Medicine 80(3). p.874-884
Abstract

PURPOSE: Determine the feasibility of 1 H-[13 C] MRS in the mouse hypothalamus using a 14.1T magnet.

METHODS: We optimized the design of a 1 H-[13 C] surface coil to maximize the signal-to-noise ratio of 1 H-[13 C] MRS in the mouse hypothalamus. With enhanced signal, 13 C accumulation in glucose metabolites was measured in a 8.7 µL voxel in the hypothalamus of 5 healthy mice during the continuous administration of [1,6-13 C2 ]glucose.

RESULTS: Accumulation of 13 C label in glucose C6 and lactate C3 was visible in the hypothalamus 11 min after glucose administration. The 13 C fractional enrichment (FE) curves of lactate C3, glutamate and glutamine C4, glutamate+glutamine C3 and C2, GABA C2, C3, and C4, and aspartate C3 were... (More)

PURPOSE: Determine the feasibility of 1 H-[13 C] MRS in the mouse hypothalamus using a 14.1T magnet.

METHODS: We optimized the design of a 1 H-[13 C] surface coil to maximize the signal-to-noise ratio of 1 H-[13 C] MRS in the mouse hypothalamus. With enhanced signal, 13 C accumulation in glucose metabolites was measured in a 8.7 µL voxel in the hypothalamus of 5 healthy mice during the continuous administration of [1,6-13 C2 ]glucose.

RESULTS: Accumulation of 13 C label in glucose C6 and lactate C3 was visible in the hypothalamus 11 min after glucose administration. The 13 C fractional enrichment (FE) curves of lactate C3, glutamate and glutamine C4, glutamate+glutamine C3 and C2, GABA C2, C3, and C4, and aspartate C3 were measured with a time resolution of 11 min over 190 min. FE time-courses and metabolic pool sizes were averaged to fit a novel one-compartment model of brain energy metabolism that incorporates the main features of the hypothalamus.

CONCLUSION: Dynamic 1 H-[13 C] MRS is able to measure in vivo brain metabolism in small and deep areas of the mouse brain such as the hypothalamus, and it can be used to calculate metabolic fluxes, including glutamatergic and GABAergic metabolism as well as the contribution of metabolic sources other than glucose.

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author
publishing date
type
Contribution to journal
publication status
published
in
Magnetic Resonance in Medicine
volume
80
issue
3
pages
874 - 884
publisher
Wiley Online Library
external identifiers
  • scopus:85041523201
  • pmid:29427382
ISSN
1522-2594
DOI
10.1002/mrm.27129
language
English
LU publication?
no
id
02ab9ea8-0247-48fe-8921-4fa1fc8de36c
date added to LUP
2019-02-22 09:13:31
date last changed
2020-01-16 03:46:33
@article{02ab9ea8-0247-48fe-8921-4fa1fc8de36c,
  abstract     = {<p>PURPOSE: Determine the feasibility of 1 H-[13 C] MRS in the mouse hypothalamus using a 14.1T magnet.</p><p>METHODS: We optimized the design of a 1 H-[13 C] surface coil to maximize the signal-to-noise ratio of 1 H-[13 C] MRS in the mouse hypothalamus. With enhanced signal, 13 C accumulation in glucose metabolites was measured in a 8.7 µL voxel in the hypothalamus of 5 healthy mice during the continuous administration of [1,6-13 C2 ]glucose.</p><p>RESULTS: Accumulation of 13 C label in glucose C6 and lactate C3 was visible in the hypothalamus 11 min after glucose administration. The 13 C fractional enrichment (FE) curves of lactate C3, glutamate and glutamine C4, glutamate+glutamine C3 and C2, GABA C2, C3, and C4, and aspartate C3 were measured with a time resolution of 11 min over 190 min. FE time-courses and metabolic pool sizes were averaged to fit a novel one-compartment model of brain energy metabolism that incorporates the main features of the hypothalamus.</p><p>CONCLUSION: Dynamic 1 H-[13 C] MRS is able to measure in vivo brain metabolism in small and deep areas of the mouse brain such as the hypothalamus, and it can be used to calculate metabolic fluxes, including glutamatergic and GABAergic metabolism as well as the contribution of metabolic sources other than glucose.</p>},
  author       = {Lizarbe, Blanca and Lei, Hongxia and Duarte, Joao M N and Lanz, Bernard and Cherix, Antoine and Gruetter, Rolf},
  issn         = {1522-2594},
  language     = {eng},
  number       = {3},
  pages        = {874--884},
  publisher    = {Wiley Online Library},
  series       = {Magnetic Resonance in Medicine},
  title        = {Feasibility of in vivo measurement of glucose metabolism in the mouse hypothalamus by 1 H-[13 C] MRS at 14.1T},
  url          = {http://dx.doi.org/10.1002/mrm.27129},
  doi          = {10.1002/mrm.27129},
  volume       = {80},
  year         = {2018},
}