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Interaction of the Fc part of IgG with Lancefield extracts of hemolytic streptococci. Strain specificity and activity

Christensen, P ; Burova, L A ; Grubb, A LU orcid ; Grubb, R ; Samuelsson, G ; Schalén, C and Svensson, M L (1979) In Acta Pathologica et Microbiologica Scandinavica. Section C, Immunology 87C(1). p.7-73
Abstract

Lancefield extracts of 19 types of group A streptococci as well as one group C and one group G strain were examined for agglutination of human red cells coated with various anti-Rh antibodies. Fourteen extracts agglutinated one or more of the coated cell samples, while five did not. The agglutination was inhibited by Fc but not by Fab fragments of human IgG. After mouse passages, three of the non-agglutinating strains acquired agglutinating capacity. At least three different reactivities were distinguished by the action of the extracts on IgG1 and IgG3 coated cells, respectively. Two of the streptococcal extracts, agglutinating the same anti-Rh coated cells, could be further differentiated in hemagglutination inhibition (HAI)... (More)

Lancefield extracts of 19 types of group A streptococci as well as one group C and one group G strain were examined for agglutination of human red cells coated with various anti-Rh antibodies. Fourteen extracts agglutinated one or more of the coated cell samples, while five did not. The agglutination was inhibited by Fc but not by Fab fragments of human IgG. After mouse passages, three of the non-agglutinating strains acquired agglutinating capacity. At least three different reactivities were distinguished by the action of the extracts on IgG1 and IgG3 coated cells, respectively. Two of the streptococcal extracts, agglutinating the same anti-Rh coated cells, could be further differentiated in hemagglutination inhibition (HAI) experiments using purified IgG3 myeloma proteins. Five selected agglutinating systems were inhibited by purified myeloma proteins of the IgG1, IgG2, and IgG4 subclasses. IgG3 proteins inhibited only two of the five HAI systems.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Antibodies, Bacterial/analysis, Erythrocytes/immunology, Hemagglutination, Hemagglutination Inhibition Tests, Humans, Immunoglobulin Fc Fragments/immunology, Immunoglobulin G/immunology, Myeloma Proteins/immunology, Streptococcus/immunology, Streptococcus pyogenes/immunology
in
Acta Pathologica et Microbiologica Scandinavica. Section C, Immunology
volume
87C
issue
1
pages
7 - 73
publisher
Wiley-Blackwell
external identifiers
  • scopus:0018342140
  • pmid:373386
ISSN
0304-1328
language
English
LU publication?
yes
id
02c17ce0-ebf0-41bb-aaf5-b8087daf9ff2
date added to LUP
2021-10-16 19:36:01
date last changed
2024-01-12 02:57:58
@article{02c17ce0-ebf0-41bb-aaf5-b8087daf9ff2,
  abstract     = {{<p>Lancefield extracts of 19 types of group A streptococci as well as one group C and one group G strain were examined for agglutination of human red cells coated with various anti-Rh antibodies. Fourteen extracts agglutinated one or more of the coated cell samples, while five did not. The agglutination was inhibited by Fc but not by Fab fragments of human IgG. After mouse passages, three of the non-agglutinating strains acquired agglutinating capacity. At least three different reactivities were distinguished by the action of the extracts on IgG1 and IgG3 coated cells, respectively. Two of the streptococcal extracts, agglutinating the same anti-Rh coated cells, could be further differentiated in hemagglutination inhibition (HAI) experiments using purified IgG3 myeloma proteins. Five selected agglutinating systems were inhibited by purified myeloma proteins of the IgG1, IgG2, and IgG4 subclasses. IgG3 proteins inhibited only two of the five HAI systems.</p>}},
  author       = {{Christensen, P and Burova, L A and Grubb, A and Grubb, R and Samuelsson, G and Schalén, C and Svensson, M L}},
  issn         = {{0304-1328}},
  keywords     = {{Antibodies, Bacterial/analysis; Erythrocytes/immunology; Hemagglutination; Hemagglutination Inhibition Tests; Humans; Immunoglobulin Fc Fragments/immunology; Immunoglobulin G/immunology; Myeloma Proteins/immunology; Streptococcus/immunology; Streptococcus pyogenes/immunology}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{7--73}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Acta Pathologica et Microbiologica Scandinavica. Section C, Immunology}},
  title        = {{Interaction of the Fc part of IgG with Lancefield extracts of hemolytic streptococci. Strain specificity and activity}},
  volume       = {{87C}},
  year         = {{1979}},
}