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Insulin stimulates ganglionic protein synthesis and reduces thymidine incorporation in support cells of the in vitro regenerating adult frog sciatic sensory neurons

Ekström, Per A R LU (1991) In Neuroscience Letters 132(2). p.183-186
Abstract

Insulin was tested for effects on crush injured, in vitro regenerating, adult frog sciatic sensory axons. A wide range of insulin concentrations (0.01-10 μg × ml-1) was found to stimulate incorporation of radioactive leucine into ganglionic protein by 50-80%. without affecting the regeneration distance. Simultaneously insulin inhibited the proliferation of the support cells at the crush region by 30%, as measured by thymidine incorporation. Experiments using compartmentalized culture dishes indicated that the proliferation inhibitory effect could be indirect and mediated by the neuronal cells. The results suggest that insulin influences the metabolism of adult peripheral neuronal cell bodies. The stimulated nerve cells could... (More)

Insulin was tested for effects on crush injured, in vitro regenerating, adult frog sciatic sensory axons. A wide range of insulin concentrations (0.01-10 μg × ml-1) was found to stimulate incorporation of radioactive leucine into ganglionic protein by 50-80%. without affecting the regeneration distance. Simultaneously insulin inhibited the proliferation of the support cells at the crush region by 30%, as measured by thymidine incorporation. Experiments using compartmentalized culture dishes indicated that the proliferation inhibitory effect could be indirect and mediated by the neuronal cells. The results suggest that insulin influences the metabolism of adult peripheral neuronal cell bodies. The stimulated nerve cells could in turn affect the proliferation of support cells in the nerve trunk. © 1991.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
In vitro, Inhibition, Insulin, Nerve regeneration, Proliferation, Protein synthesis, Sciatic nerve, Stimulation
in
Neuroscience Letters
volume
132
issue
2
pages
183 - 186
publisher
Elsevier
external identifiers
  • Scopus:0026051725
ISSN
0304-3940
DOI
10.1016/0304-3940(91)90297-7
language
English
LU publication?
yes
id
02dff964-d36d-4f53-89d0-5e8b85916e51
date added to LUP
2016-12-07 14:36:05
date last changed
2017-01-25 16:29:25
@article{02dff964-d36d-4f53-89d0-5e8b85916e51,
  abstract     = {<p>Insulin was tested for effects on crush injured, in vitro regenerating, adult frog sciatic sensory axons. A wide range of insulin concentrations (0.01-10 μg × ml<sup>-1</sup>) was found to stimulate incorporation of radioactive leucine into ganglionic protein by 50-80%. without affecting the regeneration distance. Simultaneously insulin inhibited the proliferation of the support cells at the crush region by 30%, as measured by thymidine incorporation. Experiments using compartmentalized culture dishes indicated that the proliferation inhibitory effect could be indirect and mediated by the neuronal cells. The results suggest that insulin influences the metabolism of adult peripheral neuronal cell bodies. The stimulated nerve cells could in turn affect the proliferation of support cells in the nerve trunk. © 1991.</p>},
  author       = {Ekström, Per A R},
  issn         = {0304-3940},
  keyword      = {In vitro,Inhibition,Insulin,Nerve regeneration,Proliferation,Protein synthesis,Sciatic nerve,Stimulation},
  language     = {eng},
  month        = {11},
  number       = {2},
  pages        = {183--186},
  publisher    = {Elsevier},
  series       = {Neuroscience Letters},
  title        = {Insulin stimulates ganglionic protein synthesis and reduces thymidine incorporation in support cells of the in vitro regenerating adult frog sciatic sensory neurons},
  url          = {http://dx.doi.org/10.1016/0304-3940(91)90297-7},
  volume       = {132},
  year         = {1991},
}