Museomics : Phylogenomics of the Moth Family Epicopeiidae (Lepidoptera) Using Target Enrichment
(2021) In Insect Systematics and Diversity 5(2).- Abstract
Billions of specimens can be found in natural history museum collections around the world, holding potential molecular secrets to be unveiled. Among them are intriguing specimens of rare families of moths that, while represented in morphology-based works, are only beginning to be included in genomic studies: Pseudobistonidae, Sematuridae, and Epicopeiidae. These three families are part of the superfamily Geometroidea, which has recently been defined based on molecular data. Here we chose to focus on these three moth families to explore the suitability of a genome reduction method, target enrichment (TE), on museum specimens. Through this method, we investigated the phylogenetic relationships of these families of Lepidoptera, in... (More)
Billions of specimens can be found in natural history museum collections around the world, holding potential molecular secrets to be unveiled. Among them are intriguing specimens of rare families of moths that, while represented in morphology-based works, are only beginning to be included in genomic studies: Pseudobistonidae, Sematuridae, and Epicopeiidae. These three families are part of the superfamily Geometroidea, which has recently been defined based on molecular data. Here we chose to focus on these three moth families to explore the suitability of a genome reduction method, target enrichment (TE), on museum specimens. Through this method, we investigated the phylogenetic relationships of these families of Lepidoptera, in particular the family Epicopeiidae. We successfully sequenced 25 samples, collected between 1892 and 2001. We use 378 nuclear genes to reconstruct a phylogenetic hypothesis from the maximum likelihood analysis of a total of 36 different species, including 19 available transcriptomes. The hypothesis that Sematuridae is the sister group of Epicopeiidae + Pseudobistonidae had strong support. This study thus adds to the growing body of work, demonstrating that museum specimens can successfully contribute to molecular phylogenetic studies.
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- author
- Call, Elsa LU ; Mayer, Christoph ; Twort, Victoria LU ; Dietz, Lars ; Wahlberg, Niklas LU and Espeland, Marianne
- organization
- publishing date
- 2021-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Lepidoptera, Museomics, museum sample, phylogenomics, target enrichment
- in
- Insect Systematics and Diversity
- volume
- 5
- issue
- 2
- article number
- 6
- publisher
- Oxford University Press
- external identifiers
-
- scopus:85103202945
- ISSN
- 2399-3421
- DOI
- 10.1093/isd/ixaa021
- language
- English
- LU publication?
- yes
- id
- 02f1e7d7-dc48-45ff-b7ac-0c1fcd31a200
- date added to LUP
- 2022-02-08 12:30:33
- date last changed
- 2024-05-16 07:56:28
@article{02f1e7d7-dc48-45ff-b7ac-0c1fcd31a200,
abstract = {{<p>Billions of specimens can be found in natural history museum collections around the world, holding potential molecular secrets to be unveiled. Among them are intriguing specimens of rare families of moths that, while represented in morphology-based works, are only beginning to be included in genomic studies: Pseudobistonidae, Sematuridae, and Epicopeiidae. These three families are part of the superfamily Geometroidea, which has recently been defined based on molecular data. Here we chose to focus on these three moth families to explore the suitability of a genome reduction method, target enrichment (TE), on museum specimens. Through this method, we investigated the phylogenetic relationships of these families of Lepidoptera, in particular the family Epicopeiidae. We successfully sequenced 25 samples, collected between 1892 and 2001. We use 378 nuclear genes to reconstruct a phylogenetic hypothesis from the maximum likelihood analysis of a total of 36 different species, including 19 available transcriptomes. The hypothesis that Sematuridae is the sister group of Epicopeiidae + Pseudobistonidae had strong support. This study thus adds to the growing body of work, demonstrating that museum specimens can successfully contribute to molecular phylogenetic studies. </p>}},
author = {{Call, Elsa and Mayer, Christoph and Twort, Victoria and Dietz, Lars and Wahlberg, Niklas and Espeland, Marianne}},
issn = {{2399-3421}},
keywords = {{Lepidoptera; Museomics; museum sample; phylogenomics; target enrichment}},
language = {{eng}},
month = {{03}},
number = {{2}},
publisher = {{Oxford University Press}},
series = {{Insect Systematics and Diversity}},
title = {{Museomics : Phylogenomics of the Moth Family Epicopeiidae (Lepidoptera) Using Target Enrichment}},
url = {{http://dx.doi.org/10.1093/isd/ixaa021}},
doi = {{10.1093/isd/ixaa021}},
volume = {{5}},
year = {{2021}},
}