Effects of neutral and dibasic amino acids on the in vitro release of insulin
Lernmark LU
(1972)
In Hormone Research in Paediatrics
3(1).
p.22-30
- Abstract
The insulin-releasing ability of some neutral and di-basic amino acids was studied in an in vitro system using micro-dissected pancreatic islets with a high proportion of insulin-producing β-cells. The neutral amino acids l-alanine and α-aminoisobutyric acid had no substantial effects on basal and glucose-stimulated insulin release. In contrast, 5–20 mm of the dibasic amino acid l-arginine gradually enhanced the insulin release induced by 10 mm glucose. A critical glucose concentration must be reached before l-arginine can act as an insulin secretagogue. N-methyl-l-arginine lacked the stimulatory ability. Co-transport of neutral amino acids and sodium does not elicit insulin release in adult mouse β-cells. There may, however, be a close... (More)
The insulin-releasing ability of some neutral and di-basic amino acids was studied in an in vitro system using micro-dissected pancreatic islets with a high proportion of insulin-producing β-cells. The neutral amino acids l-alanine and α-aminoisobutyric acid had no substantial effects on basal and glucose-stimulated insulin release. In contrast, 5–20 mm of the dibasic amino acid l-arginine gradually enhanced the insulin release induced by 10 mm glucose. A critical glucose concentration must be reached before l-arginine can act as an insulin secretagogue. N-methyl-l-arginine lacked the stimulatory ability. Co-transport of neutral amino acids and sodium does not elicit insulin release in adult mouse β-cells. There may, however, be a close relationship between the transport site for l-arginine and a receptor triggering insulin release.
(Less)
- author
- Lernmark
LU
- publishing date
- 1972-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Dibasic amino acids, Insulin release, Isolated islets, Mice, Neutral amino acids, Receptor sites, Transport sites
- in
- Hormone Research in Paediatrics
- volume
- 3
- issue
- 1
- pages
- 9 pages
- publisher
- Karger
- external identifiers
-
- pmid:4569911
- scopus:0015444772
- ISSN
- 1663-2818
- DOI
- 10.1159/000178254
- language
- English
- LU publication?
- no
- id
- 033e6b4c-e0c7-46f2-b6d8-df597a0945e1
- date added to LUP
- 2019-12-12 23:36:30
- date last changed
- 2025-10-14 12:18:50
@article{033e6b4c-e0c7-46f2-b6d8-df597a0945e1,
abstract = {{<p>The insulin-releasing ability of some neutral and di-basic amino acids was studied in an in vitro system using micro-dissected pancreatic islets with a high proportion of insulin-producing β-cells. The neutral amino acids l-alanine and α-aminoisobutyric acid had no substantial effects on basal and glucose-stimulated insulin release. In contrast, 5–20 mm of the dibasic amino acid l-arginine gradually enhanced the insulin release induced by 10 mm glucose. A critical glucose concentration must be reached before l-arginine can act as an insulin secretagogue. N-methyl-l-arginine lacked the stimulatory ability. Co-transport of neutral amino acids and sodium does not elicit insulin release in adult mouse β-cells. There may, however, be a close relationship between the transport site for l-arginine and a receptor triggering insulin release.</p>}},
author = {{Lernmark}},
issn = {{1663-2818}},
keywords = {{Dibasic amino acids; Insulin release; Isolated islets; Mice; Neutral amino acids; Receptor sites; Transport sites}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{22--30}},
publisher = {{Karger}},
series = {{Hormone Research in Paediatrics}},
title = {{Effects of neutral and dibasic amino acids on the in vitro release of insulin}},
url = {{http://dx.doi.org/10.1159/000178254}},
doi = {{10.1159/000178254}},
volume = {{3}},
year = {{1972}},
}