Effects of neutral and dibasic amino acids on the in vitro release of insulin
(1972) In Hormone Research in Paediatrics 3(1). p.22-30- Abstract
The insulin-releasing ability of some neutral and di-basic amino acids was studied in an in vitro system using micro-dissected pancreatic islets with a high proportion of insulin-producing β-cells. The neutral amino acids l-alanine and α-aminoisobutyric acid had no substantial effects on basal and glucose-stimulated insulin release. In contrast, 5–20 mm of the dibasic amino acid l-arginine gradually enhanced the insulin release induced by 10 mm glucose. A critical glucose concentration must be reached before l-arginine can act as an insulin secretagogue. N-methyl-l-arginine lacked the stimulatory ability. Co-transport of neutral amino acids and sodium does not elicit insulin release in adult mouse β-cells. There may, however, be a close... (More)
The insulin-releasing ability of some neutral and di-basic amino acids was studied in an in vitro system using micro-dissected pancreatic islets with a high proportion of insulin-producing β-cells. The neutral amino acids l-alanine and α-aminoisobutyric acid had no substantial effects on basal and glucose-stimulated insulin release. In contrast, 5–20 mm of the dibasic amino acid l-arginine gradually enhanced the insulin release induced by 10 mm glucose. A critical glucose concentration must be reached before l-arginine can act as an insulin secretagogue. N-methyl-l-arginine lacked the stimulatory ability. Co-transport of neutral amino acids and sodium does not elicit insulin release in adult mouse β-cells. There may, however, be a close relationship between the transport site for l-arginine and a receptor triggering insulin release.
(Less)
- author
- Lernmark LU
- publishing date
- 1972-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Dibasic amino acids, Insulin release, Isolated islets, Mice, Neutral amino acids, Receptor sites, Transport sites
- in
- Hormone Research in Paediatrics
- volume
- 3
- issue
- 1
- pages
- 9 pages
- publisher
- Karger
- external identifiers
-
- pmid:4569911
- scopus:0015444772
- ISSN
- 1663-2818
- DOI
- 10.1159/000178254
- language
- English
- LU publication?
- no
- id
- 033e6b4c-e0c7-46f2-b6d8-df597a0945e1
- date added to LUP
- 2019-12-12 23:36:30
- date last changed
- 2024-03-13 08:29:39
@article{033e6b4c-e0c7-46f2-b6d8-df597a0945e1, abstract = {{<p>The insulin-releasing ability of some neutral and di-basic amino acids was studied in an in vitro system using micro-dissected pancreatic islets with a high proportion of insulin-producing β-cells. The neutral amino acids l-alanine and α-aminoisobutyric acid had no substantial effects on basal and glucose-stimulated insulin release. In contrast, 5–20 mm of the dibasic amino acid l-arginine gradually enhanced the insulin release induced by 10 mm glucose. A critical glucose concentration must be reached before l-arginine can act as an insulin secretagogue. N-methyl-l-arginine lacked the stimulatory ability. Co-transport of neutral amino acids and sodium does not elicit insulin release in adult mouse β-cells. There may, however, be a close relationship between the transport site for l-arginine and a receptor triggering insulin release.</p>}}, author = {{Lernmark}}, issn = {{1663-2818}}, keywords = {{Dibasic amino acids; Insulin release; Isolated islets; Mice; Neutral amino acids; Receptor sites; Transport sites}}, language = {{eng}}, month = {{01}}, number = {{1}}, pages = {{22--30}}, publisher = {{Karger}}, series = {{Hormone Research in Paediatrics}}, title = {{Effects of neutral and dibasic amino acids on the in vitro release of insulin}}, url = {{http://dx.doi.org/10.1159/000178254}}, doi = {{10.1159/000178254}}, volume = {{3}}, year = {{1972}}, }