Plant secretome proteomics
Alexandersson, Erik LU ; Ali, Ashfaq LU
; Resjö, Svante
LU
and Andreasson, Erik
LU
(2013)
In Frontiers in Plant Science
4.
- Abstract
The plant secretome refers to the set of proteins secreted out of the plant cell into the surrounding extracellular space commonly referred to as the apoplast. Secreted proteins maintain cell structure and acts in signaling and are crucial for stress responses where they can interact with pathogen effectors and control the extracellular environment. Typically, secreted proteins contain an N-terminal signal peptide and are directed through the endoplasmic reticulum/Golgi pathway. However, in plants many proteins found in the secretome lack such a signature and might follow alternative ways of secretion. This review covers techniques to isolate plant secretomes and how to identify and quantify their constituent proteins. Furthermore,... (More)
The plant secretome refers to the set of proteins secreted out of the plant cell into the surrounding extracellular space commonly referred to as the apoplast. Secreted proteins maintain cell structure and acts in signaling and are crucial for stress responses where they can interact with pathogen effectors and control the extracellular environment. Typically, secreted proteins contain an N-terminal signal peptide and are directed through the endoplasmic reticulum/Golgi pathway. However, in plants many proteins found in the secretome lack such a signature and might follow alternative ways of secretion. This review covers techniques to isolate plant secretomes and how to identify and quantify their constituent proteins. Furthermore, bioinformatical tools to predict secretion signals and define the putative secretome are presented. Findings from proteomic studies and important protein families of plant secretomes, such as proteases and hydrolases, are highlighted.
(Less)
- author
- Alexandersson, Erik
LU
; Ali, Ashfaq
LU
; Resjö, Svante
LU
and Andreasson, Erik
LU
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- in
- Frontiers in Plant Science
- volume
- 4
- article number
- 4:9
- pages
- 6 pages
- publisher
- Frontiers Media S. A.
- external identifiers
-
- scopus:84878445672
- pmid:23378846
- ISSN
- 1664-462X
- DOI
- 10.3389/fpls.2013.00009
- language
- English
- LU publication?
- no
- id
- 039f3f54-27d1-4e59-9d4d-113eb20ceb55
- date added to LUP
- 2020-03-30 16:07:07
- date last changed
- 2024-05-29 11:27:48
@article{039f3f54-27d1-4e59-9d4d-113eb20ceb55,
abstract = {{<p>The plant secretome refers to the set of proteins secreted out of the plant cell into the surrounding extracellular space commonly referred to as the apoplast. Secreted proteins maintain cell structure and acts in signaling and are crucial for stress responses where they can interact with pathogen effectors and control the extracellular environment. Typically, secreted proteins contain an N-terminal signal peptide and are directed through the endoplasmic reticulum/Golgi pathway. However, in plants many proteins found in the secretome lack such a signature and might follow alternative ways of secretion. This review covers techniques to isolate plant secretomes and how to identify and quantify their constituent proteins. Furthermore, bioinformatical tools to predict secretion signals and define the putative secretome are presented. Findings from proteomic studies and important protein families of plant secretomes, such as proteases and hydrolases, are highlighted.</p>}},
author = {{Alexandersson, Erik and Ali, Ashfaq and Resjö, Svante and Andreasson, Erik}},
issn = {{1664-462X}},
language = {{eng}},
publisher = {{Frontiers Media S. A.}},
series = {{Frontiers in Plant Science}},
title = {{Plant secretome proteomics}},
url = {{http://dx.doi.org/10.3389/fpls.2013.00009}},
doi = {{10.3389/fpls.2013.00009}},
volume = {{4}},
year = {{2013}},
}