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Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium

Ravi, Krithika LU ; García-Hidalgo, Javier LU ; Brink, Daniel P. LU ; Skyvell, Martin ; Gorwa-Grauslund, Marie F. LU and Lidén, Gunnar LU (2019) In Bioresource Technology 285.
Abstract


Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g ... (More)


Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g
CDW
h)
−1
respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Gram-positive, Lignin bioconversion, Microbacterium, Syringaldehyde, Syringate demethylase
in
Bioresource Technology
volume
285
article number
121327
publisher
Elsevier
external identifiers
  • scopus:85064121207
  • pmid:30991184
ISSN
0960-8524
DOI
10.1016/j.biortech.2019.121327
project
Understanding and improving microbial cell factories through Large Scale Data-approaches
language
English
LU publication?
yes
id
03a90429-2a6d-4497-ad22-3fd04655800e
date added to LUP
2019-04-24 11:13:32
date last changed
2024-04-16 03:05:42
@article{03a90429-2a6d-4497-ad22-3fd04655800e,
  abstract     = {{<p><br>
                                                         Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g                             <br>
                            <sub>CDW</sub><br>
                                                          h)                             <br>
                            <sup>−1</sup><br>
                                                          respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks.                         <br>
                        </p>}},
  author       = {{Ravi, Krithika and García-Hidalgo, Javier and Brink, Daniel P. and Skyvell, Martin and Gorwa-Grauslund, Marie F. and Lidén, Gunnar}},
  issn         = {{0960-8524}},
  keywords     = {{Gram-positive; Lignin bioconversion; Microbacterium; Syringaldehyde; Syringate demethylase}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Bioresource Technology}},
  title        = {{Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium}},
  url          = {{http://dx.doi.org/10.1016/j.biortech.2019.121327}},
  doi          = {{10.1016/j.biortech.2019.121327}},
  volume       = {{285}},
  year         = {{2019}},
}