Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium
(2019) In Bioresource Technology 285.- Abstract
Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g ... (More)
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Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g
CDW
h)
−1
respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks.
- author
- Ravi, Krithika LU ; García-Hidalgo, Javier LU ; Brink, Daniel P. LU ; Skyvell, Martin ; Gorwa-Grauslund, Marie F. LU and Lidén, Gunnar LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Gram-positive, Lignin bioconversion, Microbacterium, Syringaldehyde, Syringate demethylase
- in
- Bioresource Technology
- volume
- 285
- article number
- 121327
- publisher
- Elsevier
- external identifiers
-
- pmid:30991184
- scopus:85064121207
- ISSN
- 0960-8524
- DOI
- 10.1016/j.biortech.2019.121327
- project
- Understanding and improving microbial cell factories through Large Scale Data-approaches
- language
- English
- LU publication?
- yes
- id
- 03a90429-2a6d-4497-ad22-3fd04655800e
- date added to LUP
- 2019-04-24 11:13:32
- date last changed
- 2024-09-17 18:13:46
@article{03a90429-2a6d-4497-ad22-3fd04655800e, abstract = {{<p><br> Hardwood lignin is made of up to 75% syringyl-units and the bioconversion of syringate and syringaldehyde is therefore of considerable interest for biological valorization of lignin. In the current study, we have isolated a syringate-consuming bacterium identified as Microbacterium sp. RG1 and characterized its growth on several lignin model compounds. Growth was observed on syringate, 3-O-methylgallate, vanillate, 4-hydroxybenzoate, ferulate and p-coumarate. Toxic aromatic aldehydes such as vanillin and syringaldehyde were converted to their respective alcohols/acids which were eventually consumed with a maximum specific uptake rate of 0.02 and 0.1 mmol (g <br> <sub>CDW</sub><br> h) <br> <sup>−1</sup><br> respectively. The isolate was further subjected to whole genome sequencing and putative genes related to the metabolism of syringyl-compounds were mapped for the first time in a Gram-positive bacterium. These findings will be of high significance when designing future host microorganisms and bioprocesses for the efficient valorization of pre-treated lignin feedstocks. <br> </p>}}, author = {{Ravi, Krithika and García-Hidalgo, Javier and Brink, Daniel P. and Skyvell, Martin and Gorwa-Grauslund, Marie F. and Lidén, Gunnar}}, issn = {{0960-8524}}, keywords = {{Gram-positive; Lignin bioconversion; Microbacterium; Syringaldehyde; Syringate demethylase}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Bioresource Technology}}, title = {{Physiological characterization and sequence analysis of a syringate-consuming Actinobacterium}}, url = {{http://dx.doi.org/10.1016/j.biortech.2019.121327}}, doi = {{10.1016/j.biortech.2019.121327}}, volume = {{285}}, year = {{2019}}, }