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Two routes for extracellular electron transfer in Enterococcus faecalis

Hederstedt, Lars LU ; Gorton, Lo LU and Pankratova, Galina LU (2020) In Journal of Bacteriology 202(7).
Abstract
Enterococcus faecalis cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox polymer (OsRP), respectively. Electron transfer mediated with ferric ions and ferric reductase activity were both found dependent on the membrane associated Ndh3 and EetA proteins, consistent with findings in Listeria monocytogenes. In contrast, electron transfer mediated with OsRP was independent of these two proteins. Quinone in the cell membrane was required for the electron... (More)
Enterococcus faecalis cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox polymer (OsRP), respectively. Electron transfer mediated with ferric ions and ferric reductase activity were both found dependent on the membrane associated Ndh3 and EetA proteins, consistent with findings in Listeria monocytogenes. In contrast, electron transfer mediated with OsRP was independent of these two proteins. Quinone in the cell membrane was required for the electron transfer with both mediators. The combined results demonstrate that extracellular electron transfer from reduced quinone to ferric ions and to OsRP occurs via different routes in the cell envelope of E. faecalis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Bacteriology
volume
202
issue
7
article number
e00725-19
pages
9 pages
publisher
American Society for Microbiology
external identifiers
  • pmid:31932308
  • scopus:85081945692
ISSN
1098-5530
DOI
10.1128/JB.00725-19
language
English
LU publication?
yes
id
03ae14ac-1e07-4c3e-ad9f-52859d13e3f7
date added to LUP
2020-04-03 09:58:16
date last changed
2020-07-08 05:14:54
@article{03ae14ac-1e07-4c3e-ad9f-52859d13e3f7,
  abstract     = {<em>Enterococcus faecalis</em> cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox polymer (OsRP), respectively. Electron transfer mediated with ferric ions and ferric reductase activity were both found dependent on the membrane associated Ndh3 and EetA proteins, consistent with findings in <em>Listeria monocytogenes</em>. In contrast, electron transfer mediated with OsRP was independent of these two proteins. Quinone in the cell membrane was required for the electron transfer with both mediators. The combined results demonstrate that extracellular electron transfer from reduced quinone to ferric ions and to OsRP occurs via different routes in the cell envelope of <em>E. faecalis</em>. },
  author       = {Hederstedt, Lars and Gorton, Lo and Pankratova, Galina},
  issn         = {1098-5530},
  language     = {eng},
  month        = {03},
  number       = {7},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Bacteriology},
  title        = {Two routes for extracellular electron transfer in <em>Enterococcus faecalis</em>},
  url          = {http://dx.doi.org/10.1128/JB.00725-19},
  doi          = {10.1128/JB.00725-19},
  volume       = {202},
  year         = {2020},
}