Two routes for extracellular electron transfer in Enterococcus faecalis
(2020) In Journal of Bacteriology 202(7).- Abstract
- Enterococcus faecalis cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox polymer (OsRP), respectively. Electron transfer mediated with ferric ions and ferric reductase activity were both found dependent on the membrane associated Ndh3 and EetA proteins, consistent with findings in Listeria monocytogenes. In contrast, electron transfer mediated with OsRP was independent of these two proteins. Quinone in the cell membrane was required for the electron... (More)
- Enterococcus faecalis cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox polymer (OsRP), respectively. Electron transfer mediated with ferric ions and ferric reductase activity were both found dependent on the membrane associated Ndh3 and EetA proteins, consistent with findings in Listeria monocytogenes. In contrast, electron transfer mediated with OsRP was independent of these two proteins. Quinone in the cell membrane was required for the electron transfer with both mediators. The combined results demonstrate that extracellular electron transfer from reduced quinone to ferric ions and to OsRP occurs via different routes in the cell envelope of E. faecalis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/03ae14ac-1e07-4c3e-ad9f-52859d13e3f7
- author
- Hederstedt, Lars LU ; Gorton, Lo LU and Pankratova, Galina LU
- organization
- publishing date
- 2020-03-11
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bacteriology
- volume
- 202
- issue
- 7
- article number
- e00725-19
- pages
- 9 pages
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:31932308
- scopus:85081945692
- ISSN
- 1098-5530
- DOI
- 10.1128/JB.00725-19
- language
- English
- LU publication?
- yes
- id
- 03ae14ac-1e07-4c3e-ad9f-52859d13e3f7
- date added to LUP
- 2020-04-03 09:58:16
- date last changed
- 2022-04-18 21:24:21
@article{03ae14ac-1e07-4c3e-ad9f-52859d13e3f7,
abstract = {{<em>Enterococcus faecalis</em> cells are known to have ferric reductase activity and the ability to transfer electrons generated in metabolism to the external environment. We have isolated mutants defective in ferric reductase activity and studied their electron transfer properties to electrodes mediated by ferric ions and an osmium complex modified redox polymer (OsRP), respectively. Electron transfer mediated with ferric ions and ferric reductase activity were both found dependent on the membrane associated Ndh3 and EetA proteins, consistent with findings in <em>Listeria monocytogenes</em>. In contrast, electron transfer mediated with OsRP was independent of these two proteins. Quinone in the cell membrane was required for the electron transfer with both mediators. The combined results demonstrate that extracellular electron transfer from reduced quinone to ferric ions and to OsRP occurs via different routes in the cell envelope of <em>E. faecalis</em>.}},
author = {{Hederstedt, Lars and Gorton, Lo and Pankratova, Galina}},
issn = {{1098-5530}},
language = {{eng}},
month = {{03}},
number = {{7}},
publisher = {{American Society for Microbiology}},
series = {{Journal of Bacteriology}},
title = {{Two routes for extracellular electron transfer in <em>Enterococcus faecalis</em>}},
url = {{http://dx.doi.org/10.1128/JB.00725-19}},
doi = {{10.1128/JB.00725-19}},
volume = {{202}},
year = {{2020}},
}