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Effects of low-molecular-weight polyols on the hydration status of the light-harvesting complex 2 from Rhodobacter sphaeroides 2.4.1

Shi, Ying ; Yu, Jie ; Liu, Yu Chen LU ; Wang, Peng and Zhang, Jian Ping (2021) In Photochemical and Photobiological Sciences 20(5). p.627-637
Abstract

Low-molecular-weight (MW) polyols are organic osmolytes influencing water activity. We have investigated the effects of polyol molecules (glycerol and sorbitol) on the optical and triplet excitation dynamics of light-harvesting complex 2 (LH2) from Rhodobacter (Rba.) sphaeroides in buffer-detergent solutions. The resonance Raman spectroscopy demonstrated that, on increasing glycerol and sorbitol volume fractions ranging from 0 to 80% (v/v) (accompanied by the decreasing water activities), the planar and all-trans conformation of carotenoids (Crts) remained unchanged, and the bacteriochlorophyll a (BChl) Qy absorption intensity decreased. The B850 fluorescence amplitude elevated in the 20–80% v/v sorbitol and 20–40% v/v... (More)

Low-molecular-weight (MW) polyols are organic osmolytes influencing water activity. We have investigated the effects of polyol molecules (glycerol and sorbitol) on the optical and triplet excitation dynamics of light-harvesting complex 2 (LH2) from Rhodobacter (Rba.) sphaeroides in buffer-detergent solutions. The resonance Raman spectroscopy demonstrated that, on increasing glycerol and sorbitol volume fractions ranging from 0 to 80% (v/v) (accompanied by the decreasing water activities), the planar and all-trans conformation of carotenoids (Crts) remained unchanged, and the bacteriochlorophyll a (BChl) Qy absorption intensity decreased. The B850 fluorescence amplitude elevated in the 20–80% v/v sorbitol and 20–40% v/v glycerol solution, but decreased in 80% v/v glycerol solution. The change of 3[Crt*–BChl] interaction bands caused by 3Crt*–BChl interaction had no obvious correlation with water activities against polyol volume fractions, which are rationalized by the water activity sensitive of C- and N-termini of protein which binding with BChls. The results suggest that Rba. sphaeroides LH2 is more sensitive to low-molecular-weight polyols compared with that of the thermophiles purple bacterium Thermochromatium (Tch.) tepidum we had investigated before. Graphic abstract: [Figure not available: see fulltext.]

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Glycerol, Hydration, Rhodobacter sphaeroides, Sorbitol, Triplet excited state
in
Photochemical and Photobiological Sciences
volume
20
issue
5
pages
11 pages
publisher
Royal Society of Chemistry
external identifiers
  • pmid:33913116
  • scopus:85105397977
ISSN
1474-905X
DOI
10.1007/s43630-021-00046-6
language
English
LU publication?
yes
id
044d65da-02b7-4089-8296-56395d782d65
date added to LUP
2021-12-17 13:39:24
date last changed
2024-06-15 22:58:24
@article{044d65da-02b7-4089-8296-56395d782d65,
  abstract     = {{<p>Low-molecular-weight (MW) polyols are organic osmolytes influencing water activity. We have investigated the effects of polyol molecules (glycerol and sorbitol) on the optical and triplet excitation dynamics of light-harvesting complex 2 (LH2) from Rhodobacter (Rba.) sphaeroides in buffer-detergent solutions. The resonance Raman spectroscopy demonstrated that, on increasing glycerol and sorbitol volume fractions ranging from 0 to 80% (v/v) (accompanied by the decreasing water activities), the planar and all-trans conformation of carotenoids (Crts) remained unchanged, and the bacteriochlorophyll a (BChl) Q<sub>y</sub> absorption intensity decreased. The B850 fluorescence amplitude elevated in the 20–80% v/v sorbitol and 20–40% v/v glycerol solution, but decreased in 80% v/v glycerol solution. The change of <sup>3</sup>[Crt*–BChl] interaction bands caused by <sup>3</sup>Crt*–BChl interaction had no obvious correlation with water activities against polyol volume fractions, which are rationalized by the water activity sensitive of C- and N-termini of protein which binding with BChls. The results suggest that Rba. sphaeroides LH2 is more sensitive to low-molecular-weight polyols compared with that of the thermophiles purple bacterium Thermochromatium (Tch.) tepidum we had investigated before. Graphic abstract: [Figure not available: see fulltext.]</p>}},
  author       = {{Shi, Ying and Yu, Jie and Liu, Yu Chen and Wang, Peng and Zhang, Jian Ping}},
  issn         = {{1474-905X}},
  keywords     = {{Glycerol; Hydration; Rhodobacter sphaeroides; Sorbitol; Triplet excited state}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{627--637}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Photochemical and Photobiological Sciences}},
  title        = {{Effects of low-molecular-weight polyols on the hydration status of the light-harvesting complex 2 from Rhodobacter sphaeroides 2.4.1}},
  url          = {{http://dx.doi.org/10.1007/s43630-021-00046-6}},
  doi          = {{10.1007/s43630-021-00046-6}},
  volume       = {{20}},
  year         = {{2021}},
}