Effects of low-molecular-weight polyols on the hydration status of the light-harvesting complex 2 from Rhodobacter sphaeroides 2.4.1
(2021) In Photochemical and Photobiological Sciences 20(5). p.627-637- Abstract
Low-molecular-weight (MW) polyols are organic osmolytes influencing water activity. We have investigated the effects of polyol molecules (glycerol and sorbitol) on the optical and triplet excitation dynamics of light-harvesting complex 2 (LH2) from Rhodobacter (Rba.) sphaeroides in buffer-detergent solutions. The resonance Raman spectroscopy demonstrated that, on increasing glycerol and sorbitol volume fractions ranging from 0 to 80% (v/v) (accompanied by the decreasing water activities), the planar and all-trans conformation of carotenoids (Crts) remained unchanged, and the bacteriochlorophyll a (BChl) Qy absorption intensity decreased. The B850 fluorescence amplitude elevated in the 20–80% v/v sorbitol and 20–40% v/v... (More)
Low-molecular-weight (MW) polyols are organic osmolytes influencing water activity. We have investigated the effects of polyol molecules (glycerol and sorbitol) on the optical and triplet excitation dynamics of light-harvesting complex 2 (LH2) from Rhodobacter (Rba.) sphaeroides in buffer-detergent solutions. The resonance Raman spectroscopy demonstrated that, on increasing glycerol and sorbitol volume fractions ranging from 0 to 80% (v/v) (accompanied by the decreasing water activities), the planar and all-trans conformation of carotenoids (Crts) remained unchanged, and the bacteriochlorophyll a (BChl) Qy absorption intensity decreased. The B850 fluorescence amplitude elevated in the 20–80% v/v sorbitol and 20–40% v/v glycerol solution, but decreased in 80% v/v glycerol solution. The change of 3[Crt*–BChl] interaction bands caused by 3Crt*–BChl interaction had no obvious correlation with water activities against polyol volume fractions, which are rationalized by the water activity sensitive of C- and N-termini of protein which binding with BChls. The results suggest that Rba. sphaeroides LH2 is more sensitive to low-molecular-weight polyols compared with that of the thermophiles purple bacterium Thermochromatium (Tch.) tepidum we had investigated before. Graphic abstract: [Figure not available: see fulltext.]
(Less)
- author
- Shi, Ying ; Yu, Jie ; Liu, Yu Chen LU ; Wang, Peng and Zhang, Jian Ping
- organization
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Glycerol, Hydration, Rhodobacter sphaeroides, Sorbitol, Triplet excited state
- in
- Photochemical and Photobiological Sciences
- volume
- 20
- issue
- 5
- pages
- 11 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- pmid:33913116
- scopus:85105397977
- ISSN
- 1474-905X
- DOI
- 10.1007/s43630-021-00046-6
- language
- English
- LU publication?
- yes
- id
- 044d65da-02b7-4089-8296-56395d782d65
- date added to LUP
- 2021-12-17 13:39:24
- date last changed
- 2024-08-11 03:45:02
@article{044d65da-02b7-4089-8296-56395d782d65, abstract = {{<p>Low-molecular-weight (MW) polyols are organic osmolytes influencing water activity. We have investigated the effects of polyol molecules (glycerol and sorbitol) on the optical and triplet excitation dynamics of light-harvesting complex 2 (LH2) from Rhodobacter (Rba.) sphaeroides in buffer-detergent solutions. The resonance Raman spectroscopy demonstrated that, on increasing glycerol and sorbitol volume fractions ranging from 0 to 80% (v/v) (accompanied by the decreasing water activities), the planar and all-trans conformation of carotenoids (Crts) remained unchanged, and the bacteriochlorophyll a (BChl) Q<sub>y</sub> absorption intensity decreased. The B850 fluorescence amplitude elevated in the 20–80% v/v sorbitol and 20–40% v/v glycerol solution, but decreased in 80% v/v glycerol solution. The change of <sup>3</sup>[Crt*–BChl] interaction bands caused by <sup>3</sup>Crt*–BChl interaction had no obvious correlation with water activities against polyol volume fractions, which are rationalized by the water activity sensitive of C- and N-termini of protein which binding with BChls. The results suggest that Rba. sphaeroides LH2 is more sensitive to low-molecular-weight polyols compared with that of the thermophiles purple bacterium Thermochromatium (Tch.) tepidum we had investigated before. Graphic abstract: [Figure not available: see fulltext.]</p>}}, author = {{Shi, Ying and Yu, Jie and Liu, Yu Chen and Wang, Peng and Zhang, Jian Ping}}, issn = {{1474-905X}}, keywords = {{Glycerol; Hydration; Rhodobacter sphaeroides; Sorbitol; Triplet excited state}}, language = {{eng}}, number = {{5}}, pages = {{627--637}}, publisher = {{Royal Society of Chemistry}}, series = {{Photochemical and Photobiological Sciences}}, title = {{Effects of low-molecular-weight polyols on the hydration status of the light-harvesting complex 2 from Rhodobacter sphaeroides 2.4.1}}, url = {{http://dx.doi.org/10.1007/s43630-021-00046-6}}, doi = {{10.1007/s43630-021-00046-6}}, volume = {{20}}, year = {{2021}}, }