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Structural characterization of the GM1 ganglioside by infrared multiphoton dissociation, electron capture dissociation, and electron detachment dissociation electrospray ionization FT-ICR MS/MS

McFarland, Melinda A; Marshall, Alan G; Hendrickson, Christopher L; Nilsson, Carol L LU ; Fredman, Pam and Månsson, Jan-Eric (2005) In Journal of the American Society for Mass Spectrometry 16(5). p.62-752
Abstract

Gangliosides play important biological roles and structural characterization of both the carbohydrate and the lipid moieties is important. The FT-ICR MS/MS techniques of electron capture dissociation (ECD), electron detachment dissociation (EDD), and infrared multiphoton dissociation (IRMPD) provide extensive fragmentation of the protonated and deprotonated GM1 ganglioside. ECD provides extensive structural information, including identification of both halves of the ceramide and cleavage of the acetyl moiety of the N-acetylated sugars. IRMPD provides similar glycan fragmentation but no cleavage of the acetyl moiety. Cleavage between the fatty acid and the long-chain base of the ceramide moiety is seen in negative-ion IRMPD but not in... (More)

Gangliosides play important biological roles and structural characterization of both the carbohydrate and the lipid moieties is important. The FT-ICR MS/MS techniques of electron capture dissociation (ECD), electron detachment dissociation (EDD), and infrared multiphoton dissociation (IRMPD) provide extensive fragmentation of the protonated and deprotonated GM1 ganglioside. ECD provides extensive structural information, including identification of both halves of the ceramide and cleavage of the acetyl moiety of the N-acetylated sugars. IRMPD provides similar glycan fragmentation but no cleavage of the acetyl moiety. Cleavage between the fatty acid and the long-chain base of the ceramide moiety is seen in negative-ion IRMPD but not in positive-ion IRMPD of GM1. Furthermore, this extent of fragmentation requires a range of laser powers, whereas all information is available from a single ECD experiment. However, stepwise fragmentation by IRMPD may be used to map the relative labilities for a series of cleavages. EDD provides the alternative of electron-induced fragmentation for negative ions with extensive fragmentation, but suffers from low efficiency as well as complication of data analysis by frequent loss of hydrogen atoms. We also show that analysis of MS/MS data for glycolipids is greatly simplified by classification of product ion masses to specific regions of the ganglioside based solely on mass defect graphical analysis.

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publication status
published
keywords
Animals, Cattle, G(M1) Ganglioside, Infrared Rays, Molecular Structure, Spectrometry, Mass, Electrospray Ionization, Spectroscopy, Fourier Transform Infrared, Comparative Study, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
in
Journal of the American Society for Mass Spectrometry
volume
16
issue
5
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:18044378372
ISSN
1044-0305
DOI
10.1016/j.jasms.2005.02.001
language
English
LU publication?
no
id
04aa4b8b-540b-4980-b7b7-f27e28312f1c
date added to LUP
2017-05-16 10:36:25
date last changed
2017-11-05 05:17:24
@article{04aa4b8b-540b-4980-b7b7-f27e28312f1c,
  abstract     = {<p>Gangliosides play important biological roles and structural characterization of both the carbohydrate and the lipid moieties is important. The FT-ICR MS/MS techniques of electron capture dissociation (ECD), electron detachment dissociation (EDD), and infrared multiphoton dissociation (IRMPD) provide extensive fragmentation of the protonated and deprotonated GM1 ganglioside. ECD provides extensive structural information, including identification of both halves of the ceramide and cleavage of the acetyl moiety of the N-acetylated sugars. IRMPD provides similar glycan fragmentation but no cleavage of the acetyl moiety. Cleavage between the fatty acid and the long-chain base of the ceramide moiety is seen in negative-ion IRMPD but not in positive-ion IRMPD of GM1. Furthermore, this extent of fragmentation requires a range of laser powers, whereas all information is available from a single ECD experiment. However, stepwise fragmentation by IRMPD may be used to map the relative labilities for a series of cleavages. EDD provides the alternative of electron-induced fragmentation for negative ions with extensive fragmentation, but suffers from low efficiency as well as complication of data analysis by frequent loss of hydrogen atoms. We also show that analysis of MS/MS data for glycolipids is greatly simplified by classification of product ion masses to specific regions of the ganglioside based solely on mass defect graphical analysis.</p>},
  author       = {McFarland, Melinda A and Marshall, Alan G and Hendrickson, Christopher L and Nilsson, Carol L and Fredman, Pam and Månsson, Jan-Eric},
  issn         = {1044-0305},
  keyword      = {Animals,Cattle,G(M1) Ganglioside,Infrared Rays,Molecular Structure,Spectrometry, Mass, Electrospray Ionization,Spectroscopy, Fourier Transform Infrared,Comparative Study,Evaluation Studies,Journal Article,Research Support, Non-U.S. Gov't,Research Support, U.S. Gov't, Non-P.H.S.},
  language     = {eng},
  number       = {5},
  pages        = {62--752},
  publisher    = {Elsevier},
  series       = {Journal of the American Society for Mass Spectrometry},
  title        = {Structural characterization of the GM1 ganglioside by infrared multiphoton dissociation, electron capture dissociation, and electron detachment dissociation electrospray ionization FT-ICR MS/MS},
  url          = {http://dx.doi.org/10.1016/j.jasms.2005.02.001},
  volume       = {16},
  year         = {2005},
}