Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase

Hao, Wei ; Li, Linsong ; Mehta, Vivek ; Lernmark, Åke LU orcid and Palmer, Jerry P. (1994) In Pancreas 9(5). p.558-562
Abstract

Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell... (More)

Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Glutamic acid decarboxylase, Insulin-dependent diabetes mellitus, β cell function
in
Pancreas
volume
9
issue
5
pages
558 - 562
publisher
Lippincott Williams & Wilkins
external identifiers
  • scopus:0028070978
  • pmid:7809009
ISSN
0885-3177
DOI
10.1097/00006676-199409000-00003
language
English
LU publication?
no
id
04e99312-0a9a-4072-b395-9757614cfe2b
date added to LUP
2019-09-11 09:07:36
date last changed
2024-03-13 08:08:25
@article{04e99312-0a9a-4072-b395-9757614cfe2b,
  abstract     = {{<p>Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.</p>}},
  author       = {{Hao, Wei and Li, Linsong and Mehta, Vivek and Lernmark, Åke and Palmer, Jerry P.}},
  issn         = {{0885-3177}},
  keywords     = {{Glutamic acid decarboxylase; Insulin-dependent diabetes mellitus; β cell function}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{5}},
  pages        = {{558--562}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{Pancreas}},
  title        = {{Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase}},
  url          = {{http://dx.doi.org/10.1097/00006676-199409000-00003}},
  doi          = {{10.1097/00006676-199409000-00003}},
  volume       = {{9}},
  year         = {{1994}},
}