Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration
Collins, A R LU and Grubb, A LU
(1998)
In Oral Microbiology and Immunology
13(1).
p.59-61
- Abstract
This study was conducted to examine the effect of cystatin D, a newly discovered salivary cysteine protease inhibitor, on human coronavirus replication. When MRC-5, human diploid lung cells, were incubated with dilutions of recombinant human cystatin D from 0.65-10 microM for 1 h prior to, during and after infection with coronavirus OC43 and 229e strains, a decrease in virus yield was observed resulting in an IC50 of 0.8 microM for both virus strains. This dose is within the normal concentration range of cystatin D, 0.12-1.9 microM found in saliva. When a single dose, 2.5 microM, was applied, cystatin inhibition of release of virus progeny was not overcome until three days post infection whereas inhibition by leupeptin, a serine and... (More)
This study was conducted to examine the effect of cystatin D, a newly discovered salivary cysteine protease inhibitor, on human coronavirus replication. When MRC-5, human diploid lung cells, were incubated with dilutions of recombinant human cystatin D from 0.65-10 microM for 1 h prior to, during and after infection with coronavirus OC43 and 229e strains, a decrease in virus yield was observed resulting in an IC50 of 0.8 microM for both virus strains. This dose is within the normal concentration range of cystatin D, 0.12-1.9 microM found in saliva. When a single dose, 2.5 microM, was applied, cystatin inhibition of release of virus progeny was not overcome until three days post infection whereas inhibition by leupeptin, a serine and cysteine protease inhibitor, was completely abrogated by two days. When cellular toxicity was measured by 3H-thymidine uptake, cystatin D did not markedly affect cell proliferation below a 10 microM dose. The results demonstrate that cystatin D is a potent inhibitor of coronavirus replication.
(Less)
- author
- Collins, A R
LU
and Grubb, A
LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Coronavirus/drug effects, Coronavirus 229E, Human, Coronavirus OC43, Human, Cystatins/pharmacology, Cysteine Proteinase Inhibitors/pharmacology, Depression, Chemical, Dose-Response Relationship, Drug, Humans, Recombinant Proteins/pharmacology, Saliva/immunology, Time Factors, Virus Cultivation/methods, Virus Replication/drug effects
- in
- Oral Microbiology and Immunology
- volume
- 13
- issue
- 1
- pages
- 59 - 61
- publisher
- Wiley
- external identifiers
-
- scopus:0032007523
- pmid:9573825
- ISSN
- 0902-0055
- DOI
- 10.1111/j.1399-302x.1998.tb00753.x
- language
- English
- LU publication?
- yes
- id
- 0528892a-165a-4826-8d98-92cbc8122625
- date added to LUP
- 2021-10-29 12:33:22
- date last changed
- 2024-02-04 07:21:06
@article{0528892a-165a-4826-8d98-92cbc8122625,
abstract = {{<p>This study was conducted to examine the effect of cystatin D, a newly discovered salivary cysteine protease inhibitor, on human coronavirus replication. When MRC-5, human diploid lung cells, were incubated with dilutions of recombinant human cystatin D from 0.65-10 microM for 1 h prior to, during and after infection with coronavirus OC43 and 229e strains, a decrease in virus yield was observed resulting in an IC50 of 0.8 microM for both virus strains. This dose is within the normal concentration range of cystatin D, 0.12-1.9 microM found in saliva. When a single dose, 2.5 microM, was applied, cystatin inhibition of release of virus progeny was not overcome until three days post infection whereas inhibition by leupeptin, a serine and cysteine protease inhibitor, was completely abrogated by two days. When cellular toxicity was measured by 3H-thymidine uptake, cystatin D did not markedly affect cell proliferation below a 10 microM dose. The results demonstrate that cystatin D is a potent inhibitor of coronavirus replication.</p>}},
author = {{Collins, A R and Grubb, A}},
issn = {{0902-0055}},
keywords = {{Coronavirus/drug effects; Coronavirus 229E, Human; Coronavirus OC43, Human; Cystatins/pharmacology; Cysteine Proteinase Inhibitors/pharmacology; Depression, Chemical; Dose-Response Relationship, Drug; Humans; Recombinant Proteins/pharmacology; Saliva/immunology; Time Factors; Virus Cultivation/methods; Virus Replication/drug effects}},
language = {{eng}},
number = {{1}},
pages = {{59--61}},
publisher = {{Wiley}},
series = {{Oral Microbiology and Immunology}},
title = {{Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration}},
url = {{http://dx.doi.org/10.1111/j.1399-302x.1998.tb00753.x}},
doi = {{10.1111/j.1399-302x.1998.tb00753.x}},
volume = {{13}},
year = {{1998}},
}