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Genetic structure of the grey side-gilled sea slug (Pleurobranchaea maculata) in coastal waters of New Zealand

Yıldırım, Yeşerin ; Anderson, Marti J. ; Hansson, Bengt LU orcid ; Patel, Selina ; Millar, Craig D. and Rainey, Paul B. (2018) In PLoS ONE 13(8).
Abstract

Pleurobranchaea maculata is a rarely studied species of the Heterobranchia found throughout the south and western Pacific–and recently recorded in Argentina–whose population genetic structure is unknown. Interest in the species was sparked in New Zealand following a series of dog deaths caused by ingestions of slugs containing high levels of the neurotoxin tetrodotoxin. Here we describe the genetic structure and demographic history of P. maculata populations from five principle locations in New Zealand based on extensive analyses of 12 microsatellite loci and the COI and CytB regions of mitochondrial DNA (mtDNA). Microsatellite data showed significant differentiation between northern and southern populations with population structure... (More)

Pleurobranchaea maculata is a rarely studied species of the Heterobranchia found throughout the south and western Pacific–and recently recorded in Argentina–whose population genetic structure is unknown. Interest in the species was sparked in New Zealand following a series of dog deaths caused by ingestions of slugs containing high levels of the neurotoxin tetrodotoxin. Here we describe the genetic structure and demographic history of P. maculata populations from five principle locations in New Zealand based on extensive analyses of 12 microsatellite loci and the COI and CytB regions of mitochondrial DNA (mtDNA). Microsatellite data showed significant differentiation between northern and southern populations with population structure being associated with previously described regional variations in tetrodotoxin concentrations. However, mtDNA sequence data did not support such structure, revealing a star-shaped haplotype network with estimates of expansion time suggesting a population expansion in the Pleistocene era. Inclusion of publicly available mtDNA sequence sea slugs from Argentina did not alter the star-shaped network. We interpret our data as indicative of a single founding population that fragmented following geographical changes that brought about the present day north-south divide in New Zealand waters. Lack of evidence of cryptic species supports data indicating that differences in toxicity of individuals among regions are a consequence of differences in diet.

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organization
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Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
13
issue
8
article number
e0202197
publisher
Public Library of Science (PLoS)
external identifiers
  • scopus:85053523053
  • pmid:30114275
ISSN
1932-6203
DOI
10.1371/journal.pone.0202197
language
English
LU publication?
yes
id
052c9ee7-d98d-4b02-8b31-43f4035a2243
date added to LUP
2018-10-18 11:08:34
date last changed
2021-09-15 06:03:14
@article{052c9ee7-d98d-4b02-8b31-43f4035a2243,
  abstract     = {<p>Pleurobranchaea maculata is a rarely studied species of the Heterobranchia found throughout the south and western Pacific–and recently recorded in Argentina–whose population genetic structure is unknown. Interest in the species was sparked in New Zealand following a series of dog deaths caused by ingestions of slugs containing high levels of the neurotoxin tetrodotoxin. Here we describe the genetic structure and demographic history of P. maculata populations from five principle locations in New Zealand based on extensive analyses of 12 microsatellite loci and the COI and CytB regions of mitochondrial DNA (mtDNA). Microsatellite data showed significant differentiation between northern and southern populations with population structure being associated with previously described regional variations in tetrodotoxin concentrations. However, mtDNA sequence data did not support such structure, revealing a star-shaped haplotype network with estimates of expansion time suggesting a population expansion in the Pleistocene era. Inclusion of publicly available mtDNA sequence sea slugs from Argentina did not alter the star-shaped network. We interpret our data as indicative of a single founding population that fragmented following geographical changes that brought about the present day north-south divide in New Zealand waters. Lack of evidence of cryptic species supports data indicating that differences in toxicity of individuals among regions are a consequence of differences in diet.</p>},
  author       = {Yıldırım, Yeşerin and Anderson, Marti J. and Hansson, Bengt and Patel, Selina and Millar, Craig D. and Rainey, Paul B.},
  issn         = {1932-6203},
  language     = {eng},
  number       = {8},
  publisher    = {Public Library of Science (PLoS)},
  series       = {PLoS ONE},
  title        = {Genetic structure of the grey side-gilled sea slug (Pleurobranchaea maculata) in coastal waters of New Zealand},
  url          = {http://dx.doi.org/10.1371/journal.pone.0202197},
  doi          = {10.1371/journal.pone.0202197},
  volume       = {13},
  year         = {2018},
}