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Covalent stabilization of alginate gel for the entrapment of living whole cells

Birnbaum, Staffan ; Pendleton, Robert ; Larsson, Per-Olof LU and Mosbach, Klaus LU (1981) In Biotechnology Letters 3. p.393-400
Abstract
Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. In Method III alginate so] was treated with periodate (to form aldehyde groups), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine.

Saccharomyces cerevisiae cells, immobilized in... (More)
Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. In Method III alginate so] was treated with periodate (to form aldehyde groups), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine.

Saccharomyces cerevisiae cells, immobilized in such stabilized gels, exhibited almost the same fermentation activity as the standard preparation. The viability of the immobilized cells was retained during the stabilization procedure as judged from their ability to multiply in the presence of nutrients.

The preparations remained stable in phosphate buffer for at least ten days without substantial release of cells. The extent of cross-linking was controlled by varying the time and the concentration of reactants, thus giving preparations ranging from beads with a thin stabilized shell to beads homogeneously stabilized. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alginate, Polyethyleneimine, Glutaraldehyde, Yeast cells
in
Biotechnology Letters
volume
3
pages
8 pages
publisher
Springer
external identifiers
  • scopus:0019843009
ISSN
0951-208X
DOI
10.1007/BF01134097
language
English
LU publication?
yes
id
057e5b0d-9b65-4a34-8e98-e462e7985acc
date added to LUP
2024-06-18 20:43:37
date last changed
2024-09-06 15:36:30
@article{057e5b0d-9b65-4a34-8e98-e462e7985acc,
  abstract     = {{Three methods were developed for preparing alginate gels containing cells that are stable in phosphate containing media. In Method I preformed alginate beads containing entrapped cells were treated with polyethyl eneimine followed by glutaraldehyde. In Method II alginate sol was treated with a carbodiimide and N-hydroxysuccinimide (to form active esters), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine. In Method III alginate so] was treated with periodate (to form aldehyde groups), mixed with cells and extruded into calcium chloride solution. The beads were subsequently cross-linked with polyethyleneimine.<br/><br/>Saccharomyces cerevisiae cells, immobilized in such stabilized gels, exhibited almost the same fermentation activity as the standard preparation. The viability of the immobilized cells was retained during the stabilization procedure as judged from their ability to multiply in the presence of nutrients.<br/><br/>The preparations remained stable in phosphate buffer for at least ten days without substantial release of cells. The extent of cross-linking was controlled by varying the time and the concentration of reactants, thus giving preparations ranging from beads with a thin stabilized shell to beads homogeneously stabilized.}},
  author       = {{Birnbaum, Staffan and Pendleton, Robert and Larsson, Per-Olof and Mosbach, Klaus}},
  issn         = {{0951-208X}},
  keywords     = {{Alginate; Polyethyleneimine; Glutaraldehyde; Yeast cells}},
  language     = {{eng}},
  pages        = {{393--400}},
  publisher    = {{Springer}},
  series       = {{Biotechnology Letters}},
  title        = {{Covalent stabilization of alginate gel for the entrapment of living whole cells}},
  url          = {{http://dx.doi.org/10.1007/BF01134097}},
  doi          = {{10.1007/BF01134097}},
  volume       = {{3}},
  year         = {{1981}},
}